= Emerging. More than 5 years before clinical availability. (24.37%, 2023)
= Expected to be clinically available in 1 to 4 years. (39.50%, 2023)
= Clinically available now. (36.13%, 2023)
MSACL 2023 : Grant

MSACL 2023 Abstract

Self-Classified Topic Area(s): Practical Training

Podium Presentation in Colton on Wednesday at 14:00 (Chair: TBA)

How To Achieve Lower Quantification Limits

Russell Grant

Russell Grant, PhD (Presenter)

Presenter Bio: Dr Grant earned a PhD in chromatographic and mass spectrometric technologies from the University of Swansea, Wales, United Kingdom. He continued his scientific training in various industrial settings, which have included senior scientist at GSK, principal scientist at Cohesive Technologies, technical director at Eli Lilly, and director of mass spectrometry at Esoterix Endocrinology.

Dr Grant has pioneered the use of direct injection technologies, chromatographic systems multiplexing, utility of automation, and new analytical platforms for application in bioanalytical applications. His research goals are focused upon improvements in speed, sensitivity, and quality of liquid chromatography with tandem mass spectrometric (LC-MS/MS) analytical systems and assays.



The answer isn't necessarily buy a newer mass spectrometry, it is attention to details and maximizing each step of the process. This one hour session will be presented in three modules.

The first module, Foundations, will focus on assessment and improvements to pipetting techniques, control of adsorptive losses and use of surrogate IS studies to assess imprecision reduction during assay development.

The second module, Formulation, will focus on system cleanliness, the fallacy of S:N, LC system dead-volume and flow rate implications, ionization efficiency enhancements, source and optics fouling, the value of signal summing + scheduled MRM and quadrupole resolution settings.

The third module, Finesse, will tie these concepts and practical solutions together to demonstrate their use for assays that have been developed, validated and deployed. Particular focus will be made to detailed establishment of “on-column” amount, controlling adsorptive losses throughout the assay, 2D-LC to decouple selectivity and sensitivity variables, selectivity and stoichiometry of reagents in worst case specimens, the consideration that while counter counter-intuitive, more degrees of selectivity (analyte losses) may be optimal for an assay

Take Home Pearls:
- How to practically improve an assay performance as defined by lower levels of quantification
- How to enhance the throughput of an LC-MS/MS assay by paying attention to the little details
- How to enhance signal responses in a mass spectrometer without sacrificing quality"

Financial Disclosure

Board Membernono
Stockyes Labcorp
IP Royaltynono

Planning to mention or discuss specific products or technology of the company(ies) listed above: