= Emerging. More than 5 years before clinical availability. (9.82%)
= Expected to be clinically available in 1 to 4 years. (12.95%)
= Clinically available now. (22.77%)
MSACL 2018 EU : Cameron

MSACL 2018 EU Abstract

Topic: Microbiology

Podium Presentation in the Ether on Thursday at 11:00 (Chair: Ed Moore)

Laser Assisted Rapid Evaporative Ionisation Mass Spectrometry: An Automated Platform for Direct-From-Culture Speciation and Clinical Sample Analysis

Simon Cameron (Presenter)
Imperial College London

Presenter Bio(s): Simon joined Prof. Zoltan Takats’s research group in October 2015 as a Research Associate in Microbial Populations and Metabonomics in the Division of Computational and Systems Medicine at Imperial College London. He received their BSc (2011) and PhD (2015) degrees from Aberystwyth University, Wales, UK. Their research interests are in the broad fields of microbiology and metabolomics, with a focus on using mass spectrometry to understand microbial metabolism and how it can be harnessed in understanding the microbiome and infection. Currently, Simon coordinates the work of the MicrobeID team at Imperial College London, which is developing rapid evaporative ionisation mass spectrometry (REIMS) as a high-throughput platform to assign taxonomic and functional classifications to microbial isolates and to the direct-from-sample profiling of mixed microbial communities.

Authors: Simon JS Cameron (1), Alvaro Perdones-Montero (1), Adam Burke (1), Kate Alexander-Hardiman (1), A Abdolrasouli (1,2), Monica Rebec (2), Zoltan Takats (1).
(1) Imperial College London, London, UK (2) Imperial College Healthcare NHS Trust, London, UK

Abstract

Rapid evaporative ionization mass spectrometry (REIMS) is a novel technique for clinical microbiology, and unlike commercially available MS platforms allows direct analysis of a culture plate or clinical diagnostic specimen. The automated and high-throughput REIMS platform previously developed now utilises a CO2 laser, rather than electrical current, for biomass heating; removing the requirement for contact to be made with the sample. This transition has allowed for an increase of over 50% in sample throughput and expands the range of sample types which can be analysed to include diagnostic specimens. This presentation will outline the current REIMS platform, the creation of a reference spectral database for over 60 microbial species, including bacteria, yeasts, and filamentous fungi, the early determination of antimicrobial susceptibilities, and direct from specimen pathogen detection.


Financial Disclosure

DescriptionY/NSource
GrantsyesWaters Corporation
Salaryno
Board Memberno
Stockno
Expensesno
IP Royaltyno

Planning to mention or discuss specific products or technology of the company(ies) listed above:

yes