= Emerging. More than 5 years before clinical availability. (9.82%)
= Expected to be clinically available in 1 to 4 years. (12.95%)
= Clinically available now. (22.77%)
MSACL 2018 EU : Darebna

MSACL 2018 EU Abstract

Topic: Microbiology

Podium Presentation in the Ether on Thursday at 11:20 (Chair: Ed Moore)

In-situ Detection of the Botulinum Neurotoxin A by Functionalized MALDI Chips

Petra Darebna (Presenter)

Authors: Petra Darebna(1,2), Petr Pompach (1,2), Jiri Dresler (3), Petr Novak (1,2)
(1) Institute of Microbiology, v.v.i., Czech Academy of Sciences, CZ (2) Faculty of Science, Charles University in Prague, CZ (3) Department of Microbiology and Biological Defence research, Military Health Institute, CZ


Botulinum neurotoxin (BoNT) is well known for causing the lethal disease botulism. The only widely accepted test for the identification of BoNTs in both clinical specimens and food is the mouse bioassay. In this study, we use MALDI chips functionalized by biotin-binding protein neutravidin prepared by ambient soft ion landing at atmospheric pressure for enrichment and detection of biotinylated peptides – products of specific endoproteinase activity of BoNT/A. The functionality of surfaces modified with biotin-binding molecules could help to drive the MALDI-TOF mass spectrometry toward its implementation into routine clinical practice.

Financial Disclosure

GrantsyesGrant Agency of Charles University
SalaryyesCzech Academy of Sciences
Board Memberno
IP Royaltyno

Planning to mention or discuss specific products or technology of the company(ies) listed above: