= Emerging. More than 5 years before clinical availability. (9.82%)
= Expected to be clinically available in 1 to 4 years. (12.95%)
= Clinically available now. (22.77%)
MSACL 2018 EU : van der Velpen

MSACL 2018 EU Abstract

Topic: Metabolomics

Podium Presentation in the Ether on Wednesday at 11:20 (Chair: Elizabeth Want)

Combined Targeted and Untargeted Strategy for Quantification of Amino Acids and Acylcarnitines in Clinically Relevant Biofluids and Tissue Lysates

Vera van der Velpen (Presenter)
Metabolomics Unit, University of Lausanne

Presenter Bio(s): Vera van der Velpen finished her BSc in Nutrition and Health and her MSc in Food Safety at Wageningen University before starting her PhD at the same university in 2009. In her PhD, she applied transcriptomics in two clinical trials to investigate the potential health effects of isoflavone supplementation in postmenopausal women. She moved on to the University of East Anglia in 2014 for her first PostDoc position, where she first applied mass spectrometry-driven metabolomics to measure exposure markers of blueberry consumption in a large clinical trial.
Since September 2017, she is working as a PostDoc at the University of Lausanne in the group of Julijana Ivanisevic, where she is using metabolomics to study the changes in brain metabolism in Alzheimer’s disease.

Authors: Vera van der Velpen (1), Tony Teav (1), Héctor Gallart-Ayala (1), Florence Mehl (1,2), Clotilde Roux (3), Shing-Chi Liu (1), Beat Riederer (4,5), Hugues Henry (6) and Julijana Ivanisevic (1)
(1) Metabolomics Platform, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland (2) Vital-IT – Swiss Institute of Bioinformatics, Lausanne, Switzerland. (3) Clinical Chemistry Service, Lausanne University Hospital, Lausanne, Switzerland (4) Proteomic Unit, Centre for Psychiatric Neuroscience, Department of Psychiatry, CHUV, Lausanne, Switzerland (5) Department of Fundamental Neurosciences, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland (6) Innovation and Development Laboratory, Clinical Chemistry Service, Lausanne University Hospital, Lausanne, Switzerland

Abstract

Amino acids and acylcarnitines are key players in energy metabolism, however, analytical methods for comprehensive and robust measurement of these compounds without derivatization or use of ion-pairing agents are scarce. We present a HILIC-based HRMS method for the absolute quantification of amino acids and acylcarnitines in a single run, while simultaneously taking advantage of HRMS in full scan mode to screen for additional derivatives (e.g. amines, hydroxylated acylcarnitines) and other polar metabolites (e.g. purines, pyrimidines, hexoses). Using a simple extraction method with internal standards, we have successfully evaluated our method with NIST plasma and examined a profile of these compounds across different biofluids (plasma, urine, CSF) and human brain tissue.


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