= Emerging. More than 5 years before clinical availability. (9.82%)
= Expected to be clinically available in 1 to 4 years. (12.95%)
= Clinically available now. (22.77%)
MSACL 2018 EU : Bagdonaite

MSACL 2018 EU Abstract

Topic: Glycomics

Podium Presentation in the Ether on Wednesday at 11:00 (Chair: Guinevere Lageveen-Kammeijer)

Global Glycoproteomic Analysis of O-glycosylation in Human Herpesviruses

Ieva Bagdonaite (Presenter)
University of Copenhagen

Authors: Ieva Bagdonaite (1) , Rickard Nordén (2), Hiren J. Joshi (1), Sarah L. King (1), Sergey Y. Vakhrushev (1), Sigvard Olofsson (2), and Hans H. Wandall (1)
(1) Copenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark (2) Department of Infectious Diseases, University of Gothenburg, Gothenburg, Sweden

Abstract

Information on site-specific O-glycosylation of viral envelope glycoproteins is generally very limited despite important functions. We present a powerful mass-spectrometry based strategy to globally identify O-glycosylation sites on viral envelope proteins of a given virus in the context of a productive infection. We successfully utilized the strategy to map O-linked glycosylation sites on several complex herpesviruses demonstrating that O-glycosylation is widely distributed on most envelope proteins. Moreover, we used genetically engineered keratinocytes lacking O-glycan elongation capacity to demonstrate that O-linked glycans are indeed important for HSV-1 biology as HSV-1 particles produced in these cells had significantly lower titers compared to wild-type keratinocytes. These tools enable wider discovery and detailed analysis of the role of site-specific O-glycosylation in virology.


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