= Emerging. More than 5 years before clinical availability. (9.82%)
= Expected to be clinically available in 1 to 4 years. (12.95%)
= Clinically available now. (22.77%)
MSACL 2018 EU : Griffin

MSACL 2018 EU Abstract

Topic: Metabolomics

Podium Presentation in the Ether on Thursday at 9:40 (Chair: Anne Bendt)

The Use of Ion Mobility Coupled with High Resolution Mass Spectrometry to Improve Separation and Identification of Lipid Biomarkers in Metabolic Diseases

Julian Griffin (Presenter)
Department of Biochemistry, University of Cambridge, Cambridge, UK & University of Cagliari, Italy

Authors: Christine Hinz (1), Sonia Liggi (1), Julia Denes (1), Antonio Murgia (1, 2), Zoe Hall (1), Ke-di Liu (1), Luigi Atzori (3), John Fjeldsted (4), and Julian L. Griffin (1, 2)
(1) Department of Biochemistry, University of Cambridge, Cambridge, UK (2) University of Cagliari, Italy, (3) University of Cagliari, Sardinia, Italy, (4) Agilent Technologies, Inc., Santa Clara, CA, USA

Abstract

A major challenge of LC-MS of complex lipid mixtures is the separation/identification of isobaric compounds, which often have similar chromatographic properties, and fragmentation data can be inconclusive. Ion mobility (IM) separates isobaric ions based on their mobilities through an inert gas, with mobility correlated to the collisional cross sections (CCSs) of molecules. Using a combination of samples and standards, and an in-house database based on mass-to-charge ratios, retention times, MS/MS spectra, and CCS values, we have used this workflow to improve the annotation of lipid species and demonstrate its use by analysing adipose tissue in an obese mouse model.


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