= Emerging. More than 5 years before clinical availability. (9.82%)
= Expected to be clinically available in 1 to 4 years. (12.95%)
= Clinically available now. (22.77%)
MSACL 2018 EU : Anjo

MSACL 2018 EU Abstract

Topic: Proteomics

Poster Presentation
Poster #
Attended on at

Mitochondrial Proteins as Parkinson’s Disease Circulatory Biomarkers – a Translational Study

Sandra Anjo (Presenter)
University of Coimbra (CNC-UC)

>> POSTER (PDF)

Presenter Bio(s): I have a PhD degree in Biosciences from the University of Coimbra. Since April 2017, I am a postdoc fellow in a project devoted to biomarker discovery in diseases that have synaptic dysfunction as a common denominator. I have been working in the neuroscience field since my masters, using interactomics and quantitative mass spectrometry (MS) to clarify the mechanisms of action of a PD-related protein. During my PhD, I further extended my interests to a more applied field, resulting in the implementation of an innovative biomarkers discovery method and preliminary data on the identification of secreted oxidative stress and cell death biomarkers using cells and animal models. My current research interests are: i) quantitative mass spectrometry applied to neuroproteomics, interatomics, and biomarker discovery and ii) oxidative Stress impact in neurodegeneration & neurodegenerative disorders.

Authors: Sandra I. Anjo (1, 2), Patrícia Valério dos Santos (3), Maria Luiza Constante Rosado (4, 5), Graça Baltazar (4), Mário Grãos (1, 6), Bruno Manadas (1
(1) Center for Neuroscience and Cell Biology, UC, Coimbra, Portugal; (2) Faculty of Medicine, UC, Coimbra, Portugal; (3) Centro Hospitalar de Setúbal, Setúbal, Portugal; (4) Faculty of Health Sciences, UBI, Covilhã, Portugal; (5) Centro Hospitalar Cova da Beira, E.P.E., Covilhã, Portugal; (6) Biocant, Biotechnology Transfer Association, Cantanhede, Portugal

Abstract

Using a translational approach (from cells secretome to plasma samples), we could identify two mitochondria-related proteins in plasma samples, which in combination lead to a powerful model with potential diagnostic value to discriminate the PD patients from matched. The analysis of secretomes from cells cultured under control or oxidative stress conditions, reveals several mitochondria-related proteins released in higher amounts under oxidative stress. This screening, performed by SWATH-MS, was translated to plasma samples from 28 control and 31 PD patients, and two of these proteins were found to be significantly changed. These proteins are associated with apoptotic mitochondrial changes, which may correspond to potential indicators of cell death and have never been reported as blood biomarkers for PD.


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