MSACL 2022 : Vinjamuri

MSACL 2022 Industry Workshop Presentation

*This Podium Presentation is occuring in the context of an Industry Workshop that starts at the time below. Its actual start time may be up to 40 minutes later, depending on order of presentation if there are multiple presentations planned by the workshop host.

Workshop Host:Agilent Technologies
Day:Wednesday April 6
Location:De Anza 3 (Track 3)

Automated Rapid Throughput Quantitative Proteomics of Amniotic Fluid Utilizing Agilent AssayMap Bravo and UHPLC-MS/MS
Anita Vinjamuri, Bachelors of Science in Chemistry
University of California Davis

Presenter Bio: Anita graduated with her Bachelors of Science in Chemistry from Texas A&M University where she worked on the optimization of ion emission for the enhanced performance of Time-of-Flight-Secondary Ion Mass Spectrometry. Following her passion for mass spectrometry, she continued her education at the University of California Davis under the supervision of Carlito Lebrilla. Here, Anita lead several mass spectrometry-based projects involving glycomic, proteomic, and glycoproteomic analysis of various biological samples including cells, feces, breast milk, serum and amniotic fluid. Anita is expecting to graduate with her PhD this year (2022) to pursue a position in bio-analytical chemistry.

Armin Oloumi, BS in Biochemistry
University of California, Davis

Presenter Bio: Armin received his BS in Biochemistry at the University of Texas at Arlington, where he worked on development of membrane protein extraction techniques for proteomics analysis in Khaledi group. He is currently pursuing his PhD in Analytical Chemistry in Lebrilla Lab at the University of California at Davis. His research is focused on utilizing MS-based tools for protein and glycan biomarker discovery.


With the emerging interest in protein biomarkers, there is a significant need for reproducible, sensitive, and time-efficient methods for quantitative analysis. Sample preparation methods for traditional proteomic biomarker discovery are often costly, time consuming and lack robustness. Collaborative efforts between the Lebrilla lab at UC Davis and Agilent Technologies introduces an automated quantitative method for targeted proteomic biomarker discovery by pairing high throughput sample preparation utilizing AssayMap Bravo with sensitive targeted detection on a UHPLC-MS/MS.

This workshop covers the complete pipeline for quantitative proteomic analysis of amniotic fluid, from rapid throughput sample preparation, data acquisition and data analysis. We will cover optimization strategies for tryptic digestion and C18 cleanup on AssayMap Bravo including efficiency, recovery, and reproducibility assessments. Additionally, reusability of C18 clean up cartridges were evaluated in aims to minimize material usage and costs. Design and construction of a comprehensive dMRM method using an Agilent 1290 UHPLC and 6495 mass spectrometer focused on targeted detection and quantitation of peptides will be overviewed, as well as the data interpretation tools for protein quantitation and validation.

For Research Use Only. Not for use in diagnostic procedures.

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