MSACL 2022 : Luo

MSACL 2022 Industry Workshop Presentation

*This Podium Presentation is occuring in the context of an Industry Workshop that starts at the time below. Its actual start time may be up to 40 minutes later, depending on order of presentation if there are multiple presentations planned by the workshop host.

Workshop Host:Thermo Fisher Scientific
Day:Thursday April 7
Location:De Anza 1 (Track 1)

Part 1 : A Therapeutic Drug Monitoring Assay of Immunosuppressants using TurboFlow LC and High-Resolution Mass Spectrometry
Ruben Y. Luo, PhD, DABCC
Stanford University

Presenter Bio: Ruben Y. Luo, PhD, DABCC, FADLM is an Assistant Professor of Pathology at Stanford University and Associate Director of Clinical Chemistry Laboratory at Stanford Health Care. He has been dedicated to innovations in translational laboratory medicine: discovery of novel diagnostic markers and innovation of diagnostic technologies. His research focuses on (1) discovering the clinical diagnostic value of molecular characteristics of protein biomarkers, and (2) developing high-resolution mass spectrometry and label-free optical sensing technologies for characterization and accurate measurement of biomarkers. He completed his clinical chemistry fellowship at University of California San Francisco. Prior to the fellowship, he had several years of work experience in the clinical diagnostic industry. He received his PhD in analytical chemistry from Stanford University, and BS in chemistry from Peking University.


An LC-HRAM-MS method with Thermo Scientific™ TurboFlow™ online SPE was developed and validated for the quantitation of immunosuppressants. Online SPE efficiently extracts analytes while removing other components in a crude supernatant, significantly simplifying sample preparation. The use of full-scan data-dependent MS2 acquisition allows for high-resolution selected-ion monitoring quantitation of the analytes, confirming the analytes using MS2 fragmentation, as well as monitoring the signal intensities of major immunosuppressant metabolites.

For Research Use Only. Not for use in diagnostic procedures.
Thermo Scientific™ Orbitrap™ mass spectrometers are intended for General Laboratory Use Only, and not for Diagnostic or Clinical Use.