MSACL 2022 : Sobolesky

MSACL 2022 Industry Workshop Presentation

*This Podium Presentation is occuring in the context of an Industry Workshop that starts at the time below. Its actual start time may be up to 40 minutes later, depending on order of presentation if there are multiple presentations planned by the workshop host.

Workshop Host:Thermo Fisher Scientific
Day:Wednesday April 6
Location:De Anza 2 (Track 2)

Part 2 : Measuring Vitamin D2 and D3 on the Cascadion™ Fully Automated LC-MS/MS System : From Installation to Result Reporting
Philip Sobolesky, PhD
Santa Clara Valley Medical Center

Presenter Bio: I received my Bachelor of Science degree in Biology from Bloomsburg University with a minor in chemistry in 2009 and my Doctor of Philosophy degree in Biomedical Sciences from the Medical University of South Carolina (MUSC) in 2014. I completed a two-year postdoctoral fellowship at MUSC in the Nephrology proteomics laboratory where I focused on learning tandem mass spectrometry and discovery of protein biomarkers for metabolic syndrome in bottlenose dolphins. I successfully completed an ABCC certified Clinical Chemistry Fellowship at the University of California, San Diego and an active member of AACC, ASMS, AACR, NRCC and MSACL. During my postdoctoral research I learned the fundamentals of mass spectrometry assay development and validated a multiple reaction monitoring mass spectrometry assay for the measurement of clinically relevant peptide modifications in serum samples. As a Clinical Chemistry Fellow at UCSD my research focused on developing a LC-MS/MS assay for marijuana markers indicating recent use in oral fluid, whole blood, and breath to be used in a study for establishing the driving impairment limit. I was also involved in identifying drugs via broad screen urine analysis using all-ion tandem mass spectrometry. Currently I am the Clinical Biochemist at Santa Clara Valley Medical Center where my responsibilities are overseeing Chemistry, Special Chemistry, and Toxicology. I am actively involved in clinical service and currently mentor future clinical laboratory scientists at San Jose State University.


This part will provide an overview of the installation and implementation of the Thermo Scientific™ Cascadion™ SM Clinical Analyzer and the Cascadion™ 25-OH Vitamin D Assay for routine testing in the clinical laboratory, detailing our experiences with the installation, validation, training, and daily use.