MSACL 2022 : Fatou

MSACL 2022 Industry Workshop Presentation

*This Podium Presentation is occuring in the context of an Industry Workshop that starts at the time below. Its actual start time may be up to 40 minutes later, depending on order of presentation if there are multiple presentations planned by the workshop host.

Workshop Host:Shimadzu
Day:Thursday April 7
Location:De Anza 3 (Track 3)

Scaling Up while Scaling Down: New Era for Plasma/Serum Proteomics
Benoit Fatou, PhD
Boston Children's Hospital, Harvard Medical School


Benoit Fatou, PhD and Hanno Steen, PhD

Department of Pathology, Boston Children’s Hospital and Harvard Medical School, Boston, MA, USA.

High throughput proteomics-based sample processing workflows have been developed, allowing for analysis of hundreds of individual neat plasma or serum samples randomized in multi-well micro plates. However, the LC/MS-based analytical pipeline requires an excellent robustness and reproducibility of the short gradients to be compatible with such large cohort studies, and to limit potential technical biases and batch effects. Given these constraints, we have been developing robust sample-sparing high throughput sample processing methods in combination with state-of-the-art instrumentation to enable large scale plasma/serum proteomic studies using submicroliter volumes of sample. We have developed a targeted and multiplexed LC/MS method, on a Shimadzu LCMS8060 triple quadrupole mass spectrometer to monitor the human classical plasma proteome in a high throughput manner (<15 min per sample). We are focusing on those classical plasma proteins needed for a comprehensive description of the immunophenotype of humans under a wide range of immune challenges such as bacterial and viral infection, inflammation on adult and newborn cohort and/or vaccination. These studies have resulted in the successful processing and analysis of ~9,000 plasma samples (and counting).