= Emerging. More than 5 years before clinical availability. (17.55%)
= Expected to be clinically available in 1 to 4 years. (42.72%)
= Clinically available now. (39.74%)
Percent of confirmed presenters indicating Clinical Use Status: 100.00%



MSACL 2019 US: Conference Program

Palm Springs, California • March 31 - April 4, 2019

With Thanks to Our Conference Sponsors:
Shimadzu Thermo
Indigo SCIEX Agilent PerkinElmer
UTAK
And Special Thanks to Our Educational Grant Sponsors:
Brian Kelly
Danyel Tacker
Amadeo Pesce

Sunday

800
1800
Badge Pickup
@ RegDesk @ Ballroom Foyer
1400
1800

Short Course
Podium

COURSE START
Clinical MS 301
A Comprehensive Review of Clinical Mass Spectrometry Technology & Techniques, including Miniaturization
Jack Henion, PhD
Level: Intermediate - Advanced
@ Mesquite D
Group: $course_group
COURSE START
Data Science 101
Breaking up with Excel: An Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Will Slade, PhD
Level: Beginner - Intermediate
@ Room 1
Group: $course_group
COURSE START
Data Science 201
Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling
Patrick Mathias, MD, PhD & Shannon Haymond, PhD
Level: Intermediate
@ Pueblo B
Group: $course_group
COURSE START
Data Science 202
Skyline: A Vendor Neutral Tool for Quantitative Mass Spectrometry
Michael MacCoss, PhD & Brendan MacLean
Level: Beginner - Advanced
@ Pueblo A
Group: $course_group
COURSE START
Forensic Tox 101
Basic Forensic Toxicology
Jarrad Wagner, Ph.D., F-ABFT, Allison Veitenheimer, Ph.D., Russell Lewis, Ph.D., F-ABFT & Robert Johnson, PhD, F-ABFT
Level: Beginner
@ Room 2
Group: $course_group
COURSE START
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Lorin Bachmann, PhD & Grace van der Gugten
Level: Beginner - Intermediate
@ Andreas
Group: $course_group
COURSE START
LC-MSMS 201
Understanding and Optimization of LC-MS/MS to Develop Successful Methods for Identification and Quantitation in Complex Matrices
Robert D. Voyksner, PhD
Level: Intermediate
@ Chino A
Group: $course_group
COURSE START
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD & Chris Shuford, PhD
Level: Intermediate
@ Chino B
Group: $course_group
COURSE START
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Brian Rappold, Matthew Crawford and Russell Grant
Level: Advanced
@ Room 3
Group: $course_group
COURSE START
Metabolomics 201
Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis
Timothy Garrett, PhD & Erin Baker, PhD
Level: Intermediate
@ Mesquite F
Group: $course_group
COURSE START
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD & Cory Bystrom, PhD
Level: Intermediate - Advanced
@ Room 4
Group: $course_group
1800
2000
Poolside Dinner
@ Poolside
2000
2100
Hospitality Lounge
@ Rocks Patio

Monday

600
900
Breakfast
@ Date
700
1800
Badge Pickup
@ RegDesk @ Ballroom Foyer
730
1130

Short Course

Continued from Sunday
Data Science 101
Breaking up with Excel: An Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Will Slade, PhD
Level: Beginner - Intermediate
@ Room 1
Group: $course_group
Continued from Sunday
Forensic Tox 101
Basic Forensic Toxicology
Jarrad Wagner, Ph.D., F-ABFT, Allison Veitenheimer, Ph.D., Russell Lewis, Ph.D., F-ABFT & Robert Johnson, PhD, F-ABFT
Level: Beginner
@ Room 2
Group: $course_group
Continued from Sunday
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Brian Rappold, Matthew Crawford and Russell Grant
Level: Advanced
@ Room 3
Group: $course_group
Continued from Sunday
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD & Cory Bystrom, PhD
Level: Intermediate - Advanced
@ Room 4
Group: $course_group
800
1200

Short Course

Continued from Sunday
Clinical MS 301
A Comprehensive Review of Clinical Mass Spectrometry Technology & Techniques, including Miniaturization
Jack Henion, PhD
Level: Intermediate - Advanced
@ Mesquite D
Group: $course_group
Continued from Sunday
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Lorin Bachmann, PhD & Grace van der Gugten
Level: Beginner - Intermediate
@ Andreas
Group: $course_group
Continued from Sunday
LC-MSMS 201
Understanding and Optimization of LC-MS/MS to Develop Successful Methods for Identification and Quantitation in Complex Matrices
Robert D. Voyksner, PhD
Level: Intermediate
@ Chino A
Group: $course_group
COURSE START
Lipidomics 101
Mass Spectrometry-based Lipidomics and Clinical Applications
Anne K Bendt, PhD, Amaury Cazenave-Gassiot, PhD, Prof Markus R Wenk, Xianlin Han, PhD & Michael Chen, MD MSc
Level: Beginner - Intermediate
@ Room 5
Group: $course_group
830
1230

Short Course

Continued from Sunday
Data Science 201
Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling
Patrick Mathias, MD, PhD & Shannon Haymond, PhD
Level: Intermediate
@ Pueblo B
Group: $course_group
Continued from Sunday
Data Science 202
Skyline: A Vendor Neutral Tool for Quantitative Mass Spectrometry
Michael MacCoss, PhD & Brendan MacLean
Level: Beginner - Advanced
@ Pueblo A
Group: $course_group
Continued from Sunday
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD & Chris Shuford, PhD
Level: Intermediate
@ Chino B
Group: $course_group
COURSE START
MALDI 103
MALDI-MS Fundamentals and its Emerging Role in Pathology and Laboratory Medicine
Mark W. Duncan, PhD & Mari L. DeMarco, PhD DABCC FACB FCACB
Level: Beginner - Intermediate
@ Mesquite G
Group: $course_group
Continued from Sunday
Metabolomics 201
Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis
Timothy Garrett, PhD & Erin Baker, PhD
Level: Intermediate
@ Mesquite F
Group: $course_group
COURSE START
Sample Prep 201
Sample Preparation and Alternative Matrices for LC-MS Assays
William Clarke, PhD & Mark Marzinke, PhD
Level: Beginner - Intermediate
@ Mesquite E
Group: $course_group
1130
1330
Short Course Lunch
@ Date
1230
1630

Short Course

COURSE START
Forensic Tox 201
Advanced Forensic Toxicology
Jarrad Wagner, Ph.D., F-ABFT, Allison Veitenheimer, Ph.D., Russell Lewis, Ph.D., F-ABFT & Robert Johnson, PhD, F-ABFT
Level: Intermediate - Advanced
@ Room 2
Group: $course_group
Continued from Monday AM
Data Science 101
Breaking up with Excel: An Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Will Slade, PhD
Level: Beginner - Intermediate
@ Room 1
Group: $course_group
Continued from Monday AM
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Brian Rappold, Matthew Crawford and Russell Grant
Level: Advanced
@ Room 3
Group: $course_group
Continued from Monday AM
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD & Cory Bystrom, PhD
Level: Intermediate - Advanced
@ Room 4
Group: $course_group
1300
1700

Short Course

COURSE START
Microbiology 201
Applications of Mass Spectrometry in Clinical Microbiology
Chris Cox, PhD, Kirk Jensen, PhD, Clifton Fagerquist, PhD, Rebecca Harris, MD
Level: Beginner - Intermediate
@ AguaCaliente
Group: $course_group
Continued from Monday AM
Clinical MS 301
A Comprehensive Review of Clinical Mass Spectrometry Technology & Techniques, including Miniaturization
Jack Henion, PhD
Level: Intermediate - Advanced
@ Mesquite D
Group: $course_group
Continued from Monday AM
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Lorin Bachmann, PhD & Grace van der Gugten
Level: Beginner - Intermediate
@ Andreas
Group: $course_group
Continued from Monday AM
LC-MSMS 201
Understanding and Optimization of LC-MS/MS to Develop Successful Methods for Identification and Quantitation in Complex Matrices
Robert D. Voyksner, PhD
Level: Intermediate
@ Chino A
Group: $course_group
Continued from Monday AM
Lipidomics 101
Mass Spectrometry-based Lipidomics and Clinical Applications
Anne K Bendt, PhD, Amaury Cazenave-Gassiot, PhD, Prof Markus R Wenk, Xianlin Han, PhD & Michael Chen, MD MSc
Level: Beginner - Intermediate
@ Room 5
Group: $course_group
1330
1730

Short Course

Continued from Monday AM
Data Science 201
Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling
Patrick Mathias, MD, PhD & Shannon Haymond, PhD
Level: Intermediate
@ Pueblo B
Group: $course_group
Continued from Monday AM
Data Science 202
Skyline: A Vendor Neutral Tool for Quantitative Mass Spectrometry
Michael MacCoss, PhD & Brendan MacLean
Level: Beginner - Advanced
@ Pueblo A
Group: $course_group
Continued from Monday AM
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD & Chris Shuford, PhD
Level: Intermediate
@ Chino B
Group: $course_group
Continued from Monday AM
MALDI 103
MALDI-MS Fundamentals and its Emerging Role in Pathology and Laboratory Medicine
Mark W. Duncan, PhD & Mari L. DeMarco, PhD DABCC FACB FCACB
Level: Beginner - Intermediate
@ Mesquite G
Group: $course_group
Continued from Monday AM
Metabolomics 201
Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis
Timothy Garrett, PhD & Erin Baker, PhD
Level: Intermediate
@ Mesquite F
Group: $course_group
Continued from Monday AM
Sample Prep 201
Sample Preparation and Alternative Matrices for LC-MS Assays
William Clarke, PhD & Mark Marzinke, PhD
Level: Beginner - Intermediate
@ Mesquite E
Group: $course_group
1700
1900
Hospitality Lounge
@ Rocks Patio
1730
1930
Dinner on Own
@ YourChoice
1800
1930
Faculty Dinner
@ San Jacinto

invite only for SciCom, Short Course instructors and CMS journal editors.

Tuesday

600
900
Breakfast
@ Date
700
2000
Badge Pickup
@ RegDesk @ Ballroom Foyer
730
1130

Short Course

Continued from Monday PM
Forensic Tox 201
Advanced Forensic Toxicology
Jarrad Wagner, Ph.D., F-ABFT, Allison Veitenheimer, Ph.D., Russell Lewis, Ph.D., F-ABFT & Robert Johnson, PhD, F-ABFT
Level: Intermediate - Advanced
@ Room 2
Group: $course_group
Continued from Monday PM
Data Science 101
Breaking up with Excel: An Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Will Slade, PhD
Level: Beginner - Intermediate
@ Room 1
Group: $course_group
Continued from Monday PM
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Brian Rappold, Matthew Crawford and Russell Grant
Level: Advanced
@ Room 3
Group: $course_group
Continued from Monday PM
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD & Cory Bystrom, PhD
Level: Intermediate - Advanced
@ Room 4
Group: $course_group
800
1200

Short Course

Continued from Monday PM
Microbiology 201
Applications of Mass Spectrometry in Clinical Microbiology
Chris Cox, PhD, Kirk Jensen, PhD, Clifton Fagerquist, PhD, Rebecca Harris, MD
Level: Beginner - Intermediate
@ AguaCaliente
Group: $course_group
COURSE START
Presentation 101
How to Maximize Your Influence Through Creating Compelling Presentations
Karen Mahooti
Level: Beginner
@ Mesquite H
Group: $course_group
Continued from Monday PM
Clinical MS 301
A Comprehensive Review of Clinical Mass Spectrometry Technology & Techniques, including Miniaturization
Jack Henion, PhD
Level: Intermediate - Advanced
@ Mesquite D
Group: $course_group
Continued from Monday PM
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Lorin Bachmann, PhD & Grace van der Gugten
Level: Beginner - Intermediate
@ Andreas
Group: $course_group
Continued from Monday PM
LC-MSMS 201
Understanding and Optimization of LC-MS/MS to Develop Successful Methods for Identification and Quantitation in Complex Matrices
Robert D. Voyksner, PhD
Level: Intermediate
@ Chino A
Group: $course_group
830
1230

Short Course

Continued from Monday PM
Data Science 201
Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling
Patrick Mathias, MD, PhD & Shannon Haymond, PhD
Level: Intermediate
@ Pueblo B
Group: $course_group
Continued from Monday PM
Data Science 202
Skyline: A Vendor Neutral Tool for Quantitative Mass Spectrometry
Michael MacCoss, PhD & Brendan MacLean
Level: Beginner - Advanced
@ Pueblo A
Group: $course_group
Continued from Monday PM
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD & Chris Shuford, PhD
Level: Intermediate
@ Chino B
Group: $course_group
Continued from Monday PM
MALDI 103
MALDI-MS Fundamentals and its Emerging Role in Pathology and Laboratory Medicine
Mark W. Duncan, PhD & Mari L. DeMarco, PhD DABCC FACB FCACB
Level: Beginner - Intermediate
@ Mesquite G
Group: $course_group
Continued from Monday PM
Metabolomics 201
Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis
Timothy Garrett, PhD & Erin Baker, PhD
Level: Intermediate
@ Mesquite F
Group: $course_group
Continued from Monday PM
Sample Prep 201
Sample Preparation and Alternative Matrices for LC-MS Assays
William Clarke, PhD & Mark Marzinke, PhD
Level: Beginner - Intermediate
@ Mesquite E
Group: $course_group
915
1000

Get the Basics : Glycomics
An overview of the fundamentals that are driving research and development.

Progress and Barriers to Developing Glycans as Clinical Diagnostics in Tissues and Biofluids
@ Room 5

Chair(s): Richard R. Drake, Ph.D. Medical University of South Carolina, MUSC Proteomics Center and Hollings Cancer Center, Charleston, SC, USA

Major alterations in glycan composition are known to occur on cell surface and circulating glycoproteins in many chronic and acute diseases. These changes affect glycoprotein folding, secretion, and cell surface interactions with receptors and immune components. These should be excellent targets as disease biomarkers and diagnostics, and indeed many current FDA approved cancer biomarkers (e.g. PSA, CA19-9, CEA, CA125) are glycoproteins or glycan antigens. Measurement of these biomarkers are all currently antibody-based assays. There are multiple approaches to using mass spectrometry-based workflows for glycan measurement in tissues and biofluids, yet there are multiple barriers to clinical implementation. The workshop will provide an overview on the biology of the targetable glycans, and a tutorial on the mass spectrometry approaches and options that are being developed, with an emphasis on understanding the basis of these barriers and possible solutions. New MALDI mass spectrometry imaging methods for tissues and solid phase serum and antibody slide arrays will be used as data examples.

1015
1100

Get the Basics : Data Science
An overview of the fundamentals that are driving research and development.

Asking Questions and Using Answers from Data
@ Room 5

Chair(s): Randy Julian, PhD, Indigo BioAutomation

So many things have been called "Data Science" that its meaning has become almost too vague to be useful. One helpful definition is that data science is the collection of tasks and tools needed to improve decision making by using insights extracted from large data sets. Data science was recently called "a two-body problem, connecting data and reality, including the forces behind the data." In the pre-computer era, statistics was the science of connecting data to reality and improving decision making with data. Today, however, the scale of the data we work with is too large to gain insight using only traditional statistical methods. Indeed, the volume and complexity of modern data sets are often so large that they need sophisticated data management tools just for storage and recall. To apply advanced mathematical and statistical methods to even a modest data set requires fluency in at least one programming language and a working understanding of many decision-making algorithms. The data science algorithm toolbox now includes everything described as machine learning. Finally, what makes data science distinct from its components is the need for deep domain knowledge. It has become evident that to gain insight into "the forces behind the data" one must be able to make informed design and analysis decisions. This session will give an overview of the components of data science, address common myths, and describe recent innovations.

1115
1200

Get the Basics : Lipidomics
An overview of the fundamentals that are driving research and development.

Lipidomics for Clinical Diagnostics: Are we ready yet?
@ Room 5

Chair(s): Anne K Bendt, PhD. Singapore Lipidomics Incubator, National University of Singapore.

Nobody questions the clinical utility of cholesterol and triglycerides, though it is becoming evident that these decades-old tests aren’t sufficient. Over the past years, powerful and sensitive mass spectrometry has enabled the analysis of thousands of lipids and their interacting partners (i.e. ‘lipidomics’) with high sensitivity and specificity. Plasma concentrations of ceramides provide risk prediction for cardiovascular complications; elevated bile acids levels in serum are an indication of abnormal cholesterol metabolism in the liver. Leading diagnostics labs have already added these novel markers on their test menu, highlighting their market potential. In this panel discussion and Q&A session, we will highlight the opportunities of mass spectrometry for lipidomic analysis of complex human samples, both as a discovery tool (i.e. towards novel markers), for validation through clinical trials, and as clinical assays in routine testing. Typical workflows including pre-analytical, analytical and post-analytical requirements with their challenges for clinical implementation will be discussed.

1230
1345

Lunch Corporate Workshop(s)

Indigo BioAutomation
@ Rm1

Automatability, Analytics, and AI – Enabling Machine Learning in the Real World

Russell Grant, PhD: VP Research and Development - LabCorp
Randall Julian, PhD: President, CEO and Founder - Indigo BioAutomation

Data science offers a wealth of techniques, approaches and philosophies which can dramatically improve the scientific and business performance of the laboratory. Getting started in a meaningful way, however, can be a challenge, given exactly that wealth. We invite you to join a discussion, in three parts, which describes a practical roadmap for getting from “right now” to “radically new”.

Great data science begins with great data. Critical factors in the design and development of an assay affords a dramatic difference in its inherent automatability. Find out how to build quality into the assay and optimize its assessment, for the best technical performance.

When is a picture actually worth a thousand words? Great assays lead to high quality and well curated data, a critical need in data science. Visualization tools such as Tableau can reveal immediate insights.

And when is a picture not enough? Effective machine learning takes place when it complements, not duplicates, the human perspective. Learn about completely orthogonal, machine learning approaches to data processing and review.

Thermo Fisher Scientific
@ Rm2

LC-MS Tools for Clinical Toxicology Research

William Clarke, PhD, MBA, DABCC
Johns Hopkins University

For clinical toxicology research, there is increasing interest in the use of dried matrices (such as blood or urine) for testing. Advantages include biologic microsamples can be easily transported, and for analytes stable desiccated at room temperature, there is an additional benefit of easier, less expensive storage. However, there are some challenges in processing dried sample spots due to the labor-intensive nature of punching and extracting sample from collection cards. We will discuss automated approaches for dried sample analysis that can mitigate these challenges. Another challenge in clinical toxicology research is that the landscape of drug abuse is constantly changing, with new substances introduced regularly. While targeted LC-MS/MS is a useful tool, it will only detect things that we know to look for. Untargeted toxicology screening using high-resolution mass spectrometry is an important, complementary tool to targeted LC-MS/MS to understand what substances are present in drug-using communities, and also to investigate test results that are not consistent with the presentation or observation of the research subject. We will discuss ways to use untargeted toxicology screening to supplement routine targeted LC-MS/MS testing.

Shimadzu
@ Rm5

LC-MS/MS-based Targeted Metabolomics as a Key Tool for the Mechanistic Understanding of Metabolic Disease

Ruth Gordillo, Ph.D. Assistant Professor at Touchstone Diabetes Center, UTSW Medical Center Director Metabolic Phenotyping Core.

Research in the Touchstone Diabetes Center focuses on both basic and clinical aspects of Type 1 and Type 2 diabetes, and questions related to the impact of diabetes and obesity on cardiovascular disease outcome, renal disease, and cancer progression. LC-tandem mass spectrometry targeted metabolomics is a fundamental tool in all of the areas of our research. Our analytical portfolio includes the exploration of sphingolipids and phospholipids, water soluble cellular metabolites, diastereomeric separations of carbohydrates, nucleotide sugars and glycosphingolipids, and therapeutic antibodies.

Published results have revealed the existence of a relationship between levels of sphingolipid species and preclinical models of disease. Similar correlations have been found in the analysis of clinical samples.

Our laboratory has also recently reported the implication of nucleoside metabolites in energy homeostasis.

This talk will include the utilization of both, liquid chromatography and supercritical fluid chromatography with ultra-fast mass spectrometry to explore in depth the metabolic phenotype of our transgenic mouse models of disease and the broader implications to human health.

1400
1415
Welcome Orientation
@ Rooms 3-4
1415

Opening Plenary Lecture


@ Rooms 3-4

1415
1500
Analysis of Exosomes from Patient Serum Samples : Proteomic, Metabolomic and Lipidomic Analyses
David Lubman
University of Michigan Medical Center

We have isolated exosomes from the serum of ten patients with locally advanced pancreatic cancer at serial time points over a course of therapy. We have explored several different methods to purify exosomes in patient serum where it is essential to remove serum proteins including albumin for proteomics studies. Exosomes were identified by using quantitative mass spectrometry. We compared the exosomal proteome of patients at different time points during treatment to healthy controls and identified 8 proteins that show global treatment-specific changes. More recently we have studied changes in the serum exosome metabolome during therapeutic treatment where specific metabolites and lipids may be markers of treatment. The differential content of exosomes during a course of therapy suggests that exosomes may provide novel insights into the development of treatment resistance and metastasis.

1500
1515
Intermission
@ Ballroom Foyer
1515
1615

State of the Science Address
@ Rooms 3-4

Presented by MSACL US Scientific Committee Topic Leads, this address will provide an overview of the applications and technologies currently being used in Clinical Labs, and a clear view of the development pipeline. It will highlight applications expected to be available in the near-future, as well as emerging applications, and key contributors. Relevant talks, posters, and people present at the congress will be identified, enabling you to optimize your learning path and more effectively target potential network connections. Whether you are new to Clinical Mass Spectrometry, or a seasoned veteran, the State of the Science address should be on your agenda.
1615
1630
Intermission
@ Ballroom Foyer
1630
1700
Poster Lightning Talks
@ Rooms 3-4

90 seconds, one slide. Up to 15 poster presentations highlighted.
1700
1730
Vendor Lightning Talks
@ Rooms 3-4

90 seconds, one slide. Up to 20 vendors highlighted.
1730
2000
Exhibits Dinner Reception
@ Exhibit Hall in Oasis 3-4
1815
1900
Meet-a-Mentor: Booth Tours
@ Exhibit Hall in Oasis 3-4

Join a mentor as they tour the booths and learn more about technologies available, what mentors are looking for during the exchange, and how they interact with the booth vendors. Increase your network and your knowledge of products and services available.
1900
2000
Troubleshooting Forum: Poster Rounds
@ Exhibit Hall in Oasis 3-4

Join the troubleshooting poster discussion to learn something new or add your two cents.
19:00 at poster #49a
What Happened to My Topiramate?
Magdalena Rajska
SPADIA LAB a.s.
19:15 at poster #49b
Troubleshooting for Morphine Interference in a Method Analyzing Drugs of Abuse in Exhaled Breath
Malin Johansson
Karolinska University Hospital Laboratory
19:30 at poster #49c
The Challenge of 1,25-dihydroxyvitamin D: Reducing Complexity of Extraction While Simultaneously Increasing Sensitivity
Kari Gurtner
Mayo Clinic
19:45 at poster #49d
On the Path to Standardized Approach of Matrix Effect, Process Efficiency and Recovery Assessment in Clinical Mass Spectrometry Testing
Dorothy Truong
LifeLabs
2000
2100

Discussion Group

Translating Proteomics at the National Institute of Diabetes and Digestive and Kidney Diseases
@ Room 1

Chair(s): Salvatore Sechi, NIDDK/NIH

During this workshop recent successes in translating proteomics from the bench side to the clinic will be discussed. New initiatives and Funding Opportunity Announcements on developing mass spectrometric assays for proteins and peptides of interest in diabetes and obesity research will also be presented.


New Funding Opportunity.Deadline April 17, 2019

Career Transitions from Academia to Industry
@ Room 3-4

Chair(s): Dustin Yaworsky, Promega Corporation

Panelists:
Dobrin Nedelkov, Isoformix
Heather O'Neill, Caris Life Sciences
Steve Castellino, GlaxoSmithKline
Daojing Wang, Newomics
Christina Chen, Beth Israel Deaconess Medical Center
Nicole Tolan, Brigham and Women’s Hospital

Have you ever wondered what careers may be available in the life sciences, biotechnology or scientific instrumentation industry? Have you considered using your scientific creativity outside the laboratory? Do you have a business or entrepreneurial acumen that is unsatisfied by scientific research? Are you ready for a change of pace? Perhaps a career transition to scientific marketing, business development, field applications or scientific sales is the change you have been looking for.

An industry career can offer valuable business and interpersonal skills development while enriching your scientific knowledge and exposure to vast scientific markets. Join members of the MSACL community for casual conversations about various industry career options for scientists. Gain insights into the types of career opportunities that may best align with your interests and goals.

This discussion will be facilitated by Dustin Yaworsky of the Promega Corporation. Dustin obtained a BA in Chemistry from the University of South Florida, PhD in Pathobiology from University of Connecticut and post-doctoral training in Pharmaceutical Sciences/Molecular Endocrinology at the University of California San Francisco. Dustin has worked for Agilent Technologies and Waters Corporation in various roles and is now Global Clinical Collaborations Manager at Promega.

2000
2200
Hospitality Lounge
@ Rocks Patio

Wednesday

645
1800
Badge Pickup
@ RegDesk @ Ballroom Foyer
645
815
Breakfast
@ Ballroom Foyer

Pre-Workshop Breakfast
700
745

Corporate Workshop(s)

Biotage
@ Rm3

Comparison of Biotage Dual Mode Extraction (ISOLUTE® HYDRO DME+) with Solid Phase Extraction (SPE) for Sample Prep in Clinical Urine Drug Testing Assays

Ben Beppler, Sr. Development and Technology Scientist
TriCore Reference Laboratories
Albuquerque, NM

While utilization of solid phase extraction (SPE) chemistries routinely provides superior cleanup over dilute and shoot techniques for clinical urine drug testing assays, hurdles exist for many labs that impede SPE implementation, including cost, complexity, and the time required to perform extractions. When assays have a large panel of drugs with multiple drug classes, compromises in wash and elution solvents to recover all of the analytes of interest can negatively impact cleanliness of traditional SPE protocols.

In this seminar, we compare the performance of a patent pending, brand new sample prep technique, Dual Mode Extraction (ISOLUTE® HYDRO DME+), against clinically validated SPE assays for opioids, benzodiazepines, and metabolites, including evaluation of in-well enzymatic hydrolysis. We have found that the Dual Mode Extraction plates provide a simple, yet robust alternative when SPE is cost-prohibitive.

Indigo BioAutomation
@ Rm4

Pre-Register

Building a Better System – ASCENT Series 4

At previous MSACL meetings, Indigo has described how the utilization of self-aware peak processing algorithms, a comprehensive quality architecture, and a streamlined, exception-based data/result review process have proven to be a successful strategy for improving both quality and throughput of LCMS analysis. More recently, we have described how the positive impacts of these batch-oriented optimizations can be significantly magnified by an additional layer of analytics, visualization, and machine learning, all of which provide comprehensive information across instruments, assays, and batches. Now, all of that has been brought together.

While we’ve been helping labs realize how ASCENT can elevate their business performance and technical efficiency, we’ve also been learning a great deal. All of those experiences have been combined with our latest innovations, to create a platform which does even more for the laboratory. Please join us for a discussion on what we’ve learned in working side-by-side with laboratories, what our system has been taught about the ways in which labs really operate, and what ASCENT Series 4 can do for your laboratory.

Thermo Fisher Scientific
@ Rm5

The Current Challenges in Drug Quantitation from a User’s View: Can Paper Spray Mass Spectrometry Fill the Gap?

Lindsey Kirkpatrick, DO, PhD
Indiana University School of Medicine, James Whitcomb Riley Hospital for Children, Pediatric Infectious Disease Fellow

Workshop abstract:
Mass spectrometry is a powerful and sensitive analytical tool, but its application to drug quantitation at the institutional level is limited by extensive sample preparation, instrument complexity, and required technician expertise. Dr. Kirkpatrick will briefly describe the challenges clinical researchers face in obtaining drug levels at the institutional level. She will discuss the principles of paper spray mass spectrometry, which eliminates extensive sample preparation and simplifies the use of mass spectrometry-based identification and quantitation for a variety of applications. She will then describe the application of the new Thermo ScientificTM VeriSpray™ PaperSpray ion source and cartridge design that can be utilized for rapid drug detection and quantitation from dried bio-fluids.

745
800
Intermission
@ Ballroom Foyer
800
845

Corporate Workshop(s)

Restek
@ Rm1

Coated Blade Spray-Mass Spectrometry: A Novel Strategy for Rapid Screening and Quantitation

Presented by
Associate Professor Dr. Kara Lynch, University of California San Francisco
Dr. German Gomez-Rios, Restek Corporation

Coated Blade Spray (CBS) is a novel sampling/sample-preparation technology that can be directly interfaced to Mass Spectrometry (MS) instrumentation for rapid screening and quantitation of small molecules present in complex matrices. First, Professor Kara Lynch presents a comparative study between a Point of Care-CUP test and CBS-MS/MS for drug screening in urine samples of chronic pain patients. Unlike the POC-CUP test, where determination of each class of drugs requires a separate assay, the CBS-MS method is able to provide data for all selected drugs and metabolites in a single analytical run. Then, Dr. Gomez-Rios introduces a collaborative effort between the University of Waterloo and the University of Toronto towards the quantitation of four immunosuppressive drugs (i.e. cyclosporine A, tacrolimus, everolimus, sirolimus) via CBS-MS/MS in blood samples of Canadian patients. A comparative study between CBS-MS/MS and the automated immunoassay showed good agreement between both methods with observed differences consistent with those formerly reported in literature.

SCIEX
@ Rm2

Pre-Register

Title: Use of MS Workflows to Support HIV Clinical Trials: A Focus on Prevention

Presenter:
Mark Marzinke, PhD, DABCC
Associate Professor, Pathology and Medicine, Johns Hopkins University School of Medicine

Abstract:
Antiretroviral drugs have long been used for treatment and management of HIV. Currently, a fixed dose, once-daily, oral formulation of two nucleoside reverse transcriptase inhibitors (tenofovir (TFV) disoproxil fumarate and emtricitabine) is US FDA-approved to prevent HIV acquisition in high-risk populations. The pharmacology of these compounds has been evaluated using liquid-chromatographic-mass spectrometric (LC-MS) approaches. This presentation will focus on workflows associated with the validation and implementation of bioanalytical assays to understand TFV pharmacology in assessing drug adherence, as well as in the interrogation of antiretroviral drugs as alternative drug delivery systems for HIV prevention.

Thomson Instrument Co
@ Rm3

Pre-Register

Sample preparation continues to be a critical factor in the quantitative measurement of urine, oral fluid and blood samples. The goal of this seminar is to discuss how to streamline the sample preparation process utilizing the Thomson eXtreme Filter VialsTM to reduce interferences from the sample matrix and increase analyte recovery. The Thomson eXtreme Filter VialsTM allows for the analysis of multiple drug classes will employing a simple dilute, filter and shoot approach; while saving time, reducing solvent usage, and alleviates the need for expensive consumables and lab equipment.

Agilent Technologies
@ Rm4

Pre-Register

Redefining High-throughput: Bringing RapidFire® to the High Resolution “Masses”

Speaker: Frederick Strathmann, Ph.D.
NMS Labs

Analytical inefficiencies have unknowingly become commonplace in the mass spectrometry laboratory. Large panels have been used in an attempt to satisfy the need for an efficient workflow with little attention on the resultant negative impact to the utilization of the analytical system as a whole. Multiplexing strategies can help to overcome this analytical bottleneck but fall short for complex chromatograms as is the case for large screening needs. In collaboration with Agilent Technologies, NMS Labs has successfully paired a RapidFire® system with a 6545 Q-TOF to demonstrate a novel workflow capable of screening over 250 compounds in less than 30 seconds per sample. As a direct comparison to existing testing, a 10 minute injection-to-injection method was reduced to less than 25 seconds injection-to-injection for a 20-fold improvement in throughput. This talk will provide an overview of the RapidFire® technology, pros and cons of the various 6545 Q-TOF acquisition methods with a rapid injection technology, and a discussion of potential applications of this exciting technology.

For Research Use Only. Not for use in diagnostic procedures.

MilliporeSigma
@ Rm5

Enhancing Accuracy: Matrix Screening, Internal Standard Selection and Sample Clean-Up Solutions for Clinical Mass Spectrometry

Speakers:
Geoffrey Rule, PhD – MilliporeSigma, Principal Scientist, R&D
Ravi Orugunty, PhD - MilliporeSigma, Principal Scientist, R&D

Why isn’t my calibration curve perfectly linear? How does my choice of matrix calibrator impact my assays? There are several reasons a calibration curve may be non-linear, and the use of a co-eluting, isotopically labelled internal standards is the first step in obtaining a linear regression across a broad concentration range. We’ll discuss how to select isotopically labelled internal standards for accurate quantification, and how to remedy non-linear calibration curves. We will also review matrix calibrators for bioanalytical methods, and how our Cerilliant Certified Reference Materials in matrix can help reduce variability. Lastly, we’ll touch on sample preparation options for clinical testing methods, including the use of our HybridSPE phospholipid and protein removal plates for automated high-throughput determination of a 25-hydroxy vitamin D in serum.

845
900
Intermission
@ Ballroom Foyer

Scientific Session 1

Track 1
Pueblo

MALDI Imaging
Chair: Richard Drake

Track 2
Room 1

Practical Training : Results of a Survey on Diagnostic Laboratory Metadata QA Practice
Chair: Joshua Hayden

Track 3
Room 2

Breath in Breath out: Analysis of non-Invasive Matrices
Chair: Loralie Langman

Track 4
Room 3

New Technologies and Approaches in Metabolomics
Chair: Erin Baker

Track 5
Room 4

Proteomics: Discovery/Translation
Chair: Christopher Shuford

Track 6
Room 5

Wrangling Raw Data
Chair: Jay Johnson

900
920
MALDI Imaging MS: Transforming Drug Discovery and Development Through Molecular Images of Biological Systems
Steve Castellino
GlaxoSmithKline
A Survey of Mass Spectrometry Quality Assurance Practices in Clinical Laboratories
Joshua Hayden
Norton Healthcare
Cannabinoid Concentrations in Whole Blood, Oral Fluid, and Breath After Recent Use
Jacqueline Hubbard
University of California, San Diego
Sub-Microliter Metabolomics via Inductive Triboelectric Nanogenerator Mass Spectrometry
Yafeng Li
Georgia Institute of Technology
A Proteomic Predictor of Lipoprotein Function and Coronary Artery Disease: From Conception to Launch
Timothy Collier
Quest Diagnostics - Cleveland HeartLab
Impact of Discretized Intensities on Accuracy and Precision of LC-MS/MS Results
Fred Lytle
Indigo BioAutomation, Inc.
920
940
...
Keynote
...
...
Extended Session
...
A Novel Derivatization Method for High-Sensitivity LC-MS/MS Analysis of Aromatic Compounds
Y. Ruben Luo
University of California at San Francisco
Rapid Bile Acid Isomer Separation with Ion Mobility-Mass Spectrometry
Robin Kemperman
University of Florida
Mass Spectrometry-based Quantitative Diagnostic Test of Allograft Kidney using Targeted Proteome Profiling of FFPE Specimens
Kunhong Xiao
University of Pittsburgh
Chemical Fingerprints of Biofluids. Building Tandem Mass Spectral Libraries of Recurrent Spectra
Yamil Simón-Manso
National Institute of Standards and Technology
940
1000
...
Keynote
...
...
Extended Session
...
Monitoring of Acetone and Short Chain Fatty Acids Levels in Mouth and Nose Breath following Fasting and Re-Feeding with Selected Ion Flow Tube Mass Spectrometry
Ilaria Belluomo
Imperial College London
Development of a 18-plex UPLC-MS/MS Assay for Newborn Screening of Lysosomal Storage Disorders and Other Treatable Genetic Diseases
Xinying Hong
University of Washington
Development of a Proteomic Plasma Biomarker Panel for Hypertrophic Cardiomyopathy
Wendy Heywood
University College London
Improving the Sensitivity and Accuracy of Quantitative Protein Biomeasure Mass Spectrometry Through the Use of a SRM Transition Summing Technique
Jay Johnson
Pfizer Worldwide Research and Development
1000
1100
Exhibits & Poster Session
@ Exhibit Hall in Oasis 3-4

Positions A to be attended.

Scientific Session 2

Track 1
Pueblo

New Developments in MS Imaging Technologies
Chair: Guanshi Zhang

Track 2
Room 1

Practical Training : Development of a Quantitative Protein Assay
Chair: J Grace Van Der Gugten

Track 3
Room 2

Vitamins: Analytical gains for clinical pains
Chair: Joesph Wiencek

Track 4
Room 3

Metabolic profling
Chair: Adam Rosebrock

Track 5
Room 4

Proteomics: Discovery/Translation II
Chair: Michael Lassman

Track 6
Room 5

Data Science Keynote
Chair: Shannon Haymond

1100
1120
Practical Method for Molecular Imaging with Single-Cell Resolution and Very High Speed
Marvin Vestal
SimulTOF Systems of Virgin Instruments Corp
View Slides (PDF)

Part I: Digest Optimization and Quality Control for Proteomic Assays
Timothy Collier
Quest Diagnostics - Cleveland HeartLab
Addl Info (PDF)

Association of Free and Total 25 Hydroxy Vitamin D with Biomarkers of Polycystic Ovary Syndrome
Mark Kushnir
ARUP Institute for Clinical & Experimental Patholo
Metabolomic Profiling of a Large Cohort of Glioblastoma Stem Cell Lines Identifies Pathways Associated with Clinical Outcome
Olga Zaslaver
University of Toronto
Clinical Proteomics Enters Clinical Trials: A Generic GCP-compliant Workflow for the Routine Analysis of FFPE Tissue Samples by Quantitative Mass Spectrometry
Axel Ducret
F. Hoffmann-La Roche Ltd
Towards Automated Quality Assessment and Analysis of Targeted Mass Spectrometry Data using Machine Learning
Shadi Eshghi
Genenech
1120
1140
A Combination Analysis of DESI-MSI with Laser Capture Microdissection using Same Tissue Section
Emine Kazanc
Imperial College of London
Developing a Protein LC-MS/MS Assay: A Small Molecule Girl Goes Big! (Or, if I can do it, so can you!)
Grace van der Gugten
St Paul's Hospital
View Slides (PDF)

Vexed by Vitamins-Conform, Confirm or Create?
Hemamalini Ketha
Laboratory Corporation of America Holdings
Vascular Biomarkers to Predict Response to Exercise in Alzheimer’s Disease
Danni Li
University of Minnesota
Nanoliter-Scale Oir-Air-Droplet Chip-based Single Cell Proteomics Analysis
Catherine CL Wong
CPMMR, Health Science Center, Peking University

CANCELLED - replaced with
MS Protein Tests for the Clinical Laboratory
Dobrin Nedelkov (Isoformix)

...
Keynote
...
1140
1200
Multimodal Imaging Mass Spectrometry Technologies to Visualize Staphylococcal Molecular Adaptation within the Infectious Microenvironment
William Perry
Vanderbilt University
...
Extended Session
...
Strategies for Developing High-Throughput Mass Spectrometry Analyses of 25-Hydroxyvitamin D
Richard Yost
University of Florida
Nitisinone: Short and Long Term Effects on Tyrosine Metabolites in Alkaptonuria and Unmasking of the Ochronotic Pathway
Anna Milan
Royal Liverpool University Hospital
Quantitation of Intact Proteins in Human Plasma using Top-Down Parallel Reaction Monitoring-MS
Daojing Wang
Newomics Inc.
...
Keynote
...
1200
1300
Lunch
@ Exhibit Hall in Oasis 3-4
1200
1300
Clinical MS in Europe Open Forum
@ Exhibit Hall in Oasis 3-4

Meet at Booth #42-44

Meet the MSACL EU Scientific Committee Chair, Elizabeth Want, and otherswith a European perspective of clinical mass spectrometry. Enjoy a toast to kick off the MSACL 2019 EU planning season!

Scientific Session 3

Track 1
Pueblo

Keynote : Glycans in Tissue Imaging
Chair: Steve Castellino

Track 2
Room 1

Practical Training : Resolving Non-Specific Binding in qLC-MSMS
Chair: Jack Henion

Track 3
Room 2

Current MS advances for pain management: moving beyond the urine drug screen
Chair: Sara Love

Track 4
Room 3

Multiomics
Chair: Tim Garrett

Track 5
Room 4

MALDI and Intact Protein Analysis
Chair: Michael MacCoss

Track 6
Room 5

MS to Characterize Hosts and Pathogens
Chair: Rebecca Harris

1300
1320
Application of N-Glycan MALDI MS Imaging to Identify Cancer Biomarker Signatures in FFPE Tissues and Biofluids
Richard Drake
Medical University of South Carolina
Non-Specific Binding in LC-MS/MS Bioanalysis: What is it and How to Deal with It?
Jack Henion
Advion, Inc
View Slides (PDF)

A High Resolution Mass Spectrometry Method for Detecting a Comprehensive Pain-Management Drug Panel in Oral Fluid
Adina Badea
University of California, San Francisco
A Multi-omic Investigation of Molecular Signatures Indicative of Preeclampsia and Gestational Diabetes
Melanie Odenkirk
North Carolina State University
Diagnosis of Hemoglobinopathy and Beta Thalassemia by 21 Tesla Fourier Transform Ion Cyclotron Resonance MS and Top-Down MS/MS of Hemoglobin from Blood
Lidong He
University of Virginia
Mapping Pathogenesis: A Systems-Level Investigation of GBS Meningitis
Anaamika Campeau
UC San Diego
1320
1340
...
Keynote
...
...
Extended Session
...
MALDI-MS-based Validation of a 110 SNV Pharmacogenomic Panel for Pain Management Providers
Grace Williams
Dartmouth-Hitchcock Medical Center
Investigating the Skin Metabolome using Untargeted Metabolomics and the Initial Development Towards Non-Invasive Drug Monitoring
Alan Jarmusch
University of California, San Diego
Combining MALDI-TOF Serum Profiling and Top-Down Protein Analysis with 21 T FT-ICR Mass Spectrometry for Proteoform Identification
Nathan Kaiser
Biodesix
Rapid Determination of Methicillin-Resistant Staphylococcus Aureus by MALDI-TOF MS and Machine Learning
Taojunfeng Su
Weill Cornell Medicine

CANCELLED - replaced with
A Clinical Assay for Botulinum Neurotoxins through Mass Spectrometric Detection
Kaitlin Hoyt (CDC)
also presented as Poster #32d

1340
1400
...
Keynote
...
...
Extended Session
...
Comparison of a Coated Blade Spray - Mass Spectrometry (CBS-MS) Method vs a Point of Care (POC- CUP) Test for the Drug Screening in Chronic Pain Patients
Shirin Hooshfar
University of California San Francisco
Loss of SETD2 Induces a Metabolic Switch in Renal Cell Carcinoma Cell Lines Toward Enhanced Oxidative Phosphorylation
Haiwei Gu
Arizona State University
Utilizing Protein Signatures from Heterogeneous Cell Populations in a Murine Model for Diagnosis of Ovarian Cancer
Melissa Galey
University of Illinois at Chicago
Top-Down Analysis of the B-Subunit of Shiga Toxin from Escherichia coli using MALDI-TOF-TOF Mass Spectrometry: Asparagine and the Aspartic Acid Effect Mechanism
Clifton Fagerquist
Agricultural Research Service, WRRC, USDA
1400
1500
Exhibits & Poster Session
@ Exhibit Hall in Oasis 3-4

Position B to be attended.
1415
1500
Meet-a-Mentor: Poster Tours 1
@ Exhibit Hall in Oasis 3-4

Join a mentor as they tour the posters. Learn more about what mentors are looking for in a poster, and how they engage the presenter.

Scientific Session 4

Track 1
Pueblo

Environmental Contaminants
Chair: Roy Gerona

Track 2
Room 1

Practical Training : Interpretation of Urine Drug Testing Results for Pain Management
Chair: Alec Saitman

Track 3
Room 2

New Analytical Approaches to Standard Analytes
Chair: Jane Dickerson

Track 4
Room 3

New Methods in Metabolomics
Chair: Danni Li

Track 5
Room 4

Proteomic Applications to Chronic Kidney Disease
Chair: Tim Collier

Track 6
Room 5

Keynote: Maximizing the Performance of MALDI-TOF MS-based Microbial Characterization
Chair: Clifton Fagerquist

1500
1520
Mass Spectrometry: Applications to the Hospital Blood Bank
Joesph Wiencek
University of Virginia School of Medicine
Interpretation of Urine Drug Testing Results for Pain Management
Alec Saitman
Providence Regional Laboratories
View Slides (PDF)

MS & MS: Micro Sampling & Mass Spectrometry
Matthew Crawford
LabCorp
Scanning the Stable Isotopic Structure of Molecules by Tandem Mass Spectrometry
Cajetan Neubauer
California Institute of Technology
Proteomic Genotyping: When Quantitation Doesn’t Matter, But Quality Still Does
Christopher Shuford
Laboratory Corporation of America
Maximizing the Performance of MALDI-TOF MS-based Microbial Characterization: Challenges, Strategies, and Successes Beyond Species-level and Pure Culture Applications
Todd Sandrin
Arizona State University
1520
1540
Analysis of Urinary Biomarkers of Exposure to Volatile Organic Compounds from the Population Assessment of Tobacco and Health (PATH) Study (Wave 1)
Victor De Jesus
Centers for Disease Control and Prevention
...
Extended Session
...
Multi-Point Internal Calibration: IntrinsiX LC-MS/MS Analysis of Methotrexate
Melissa Hoffman
University of California, San Diego
A Novel Approach for the Classification and Detection of Pathological Pain
Meritxell Deulofeu Figueras
University of Girona
Apolipoprotein L1 is Associated with Larger HDL Particles in CKD
Andy Hoofangle
Unversity of Washington
...
Keynote
...
1540
1600
Environmental Antibiotic Exposure in Pregnancy
Zheng Cao
Beijing Obstetrics & Gynecology Hospital
View Slides (PDF)

...
Extended Session
...
The Impact of Remote Monitoring in Pediatric Renal Transplant Patients: Multiplexed Quantification of Immunosuppressants and Creatinine by LC-MS/MS
Jane Dickerson
Seattle Children's Hospital
View Slides (PDF)

Novel Headspace Sorptive Extraction on Urinary Volatile Organic Compounds for Oesophago-Gastric Cancer Detection
Qing Wen
Imperial College London
Development of a Parallel Reaction Monitoring Workflow for Biomarker Verification in Chronic Kidney Disease
Angela Mc Ardle
Cedars Sinai Medical Center
...
Keynote
...
1600
1730
Exhibits & Appetizers
@ Exhibit Hall in Oasis 3-4
1615
1715
Meet-a-Mentor: Office Hours
@ Exhibit Hall in Oasis 3-4

Have a question from the congress that you have been itching to ask? Or a problem from work that you want to get feedback on? Or feedback on the congress? Sign up at the registration desk for 15-min blocks to share time and ideas with a mentor in a relaxed setting.
1615
1715
Troubleshooting Forum: Poster Rounds
@ Exhibit Hall in Oasis 3-4

Join the troubleshooting poster discussion to learn something new or add your two cents.
16:15 at poster #50a
Humidity Fluctuation Beyond the Manufacture’s Acceptable Range in a Clinical Mass Spectrometry Laboratory
Yuzi Zheng
Cleveland Clinic
16:30 at poster #50b
The Challenges Associated with the Development of a Clinically Viable Quantitative Insulin Analog Assay
Adam Girtman
Mayo Clinic
16:45 at poster #50c
Low Extraction Efficiency in Liquid-Liquid Extraction and Quantification of Nicotine, Opioids and Cannabinoids from Plasma, Brain, and Placental Tissue
Dominique Figueroa
University of Maryland
17:00 at poster #50d
Internal Standards for Mass Spectrometry: When Gravimetric Analysis is Not Enough
Michael Gelb
Univ. of Washington
1715
1730
Plenary Reception
@ Ballroom Foyer
1730

Distinguished Contribution Award Plenary Lecture


@ Rooms 3-4
Chair: Distinguished Contribution Award Committee

1730
1830

On the Award

Immunotherapy of Cancer and the Role of Mass Spectrometry; An Overview and New Results
Donald Hunt
University of Virginia

This lecture will describe how the immune system works to keep us free of cancer most of the time and how cancer cells can eventually learn to escape the immune system. Also described will be a number of scientific breakthroughs that have occurred in the last 6 years that clearly suggest that the immune system can be re-educated and upregulated to cure even late stage cancer. Science magazine labeled cancer immunotherapy as the breakthrough event of the year in 2013 and tremendous progress has been made since then. James Allison won the 2015 Lasker Award and shared the 2018 Nobel Prize in Medicine with Tasuku Honjo for their work to reactivate the immune system to fight cancer. This lecture will try capture some this excitement and also pinpoint some of the contributions that the Hunt group has made to the field over the past 25 yrs.

1830
2030
Hospitality Lounge
@ Rocks Patio
1830
2200
FREE EVENING / OFF-SITE MEETINGS / DINNER ON OWN
@ YourChoice

Haven't decided on a dinner location yet? Head to the Hospitality Lounge at Rocks Patio to meet up with old friends or make new ones over drinks and light appetizers before heading to dinner in Palm Springs.

Thursday

745
815
Breakfast
@ Ballroom Foyer

Have breakfast at a workshop.
800
845

Corporate Workshop(s)

Waters
@ Rm1

Legal medicinal and recreational marijuana use is on the rise in the United States and around the world. This is accompanied by growing concerns of the effect of marijuana on driving performance and the need to develop methods that can detect THC and its metabolites following recent use. In this workshop, Dr. Jacqueline Hubbard from UC San Diego will discuss the development and validation of liquid chromatography tandem mass spectrometry (LC-MS/MS) assays to detect up to ten cannabinoid metabolites in whole blood, oral fluid, and exhaled breath.

Guest Speaker- Jacqueline Hubbard, UCSD

We are not pre-registering for the workshop, but seats are limited. Arrive early to secure your seat.

Ceres Nanosciences
@ Rm2

Development and Implementation of a Nanotrap® Particle Enrichment Method for Deeper Mass Spectrometry-Based Clinical Proteomics

Kirk C Hansen, Ph.D., Director of the University of Colorado School of Medicine Biological Mass Spectrometry Facility

Sample preparation methods for conventional proteomic biomarker discovery and targeted MRM assays are often labor- and time-intensive and are challenged by the low abundance of the analytes of interest. Ceres’ Nanotrap® particle technology offers a straightforward and effective approach to sample preparation and biomarker discovery. Unlike other sample preparation methods, Nanotrap® particles utilize affinity capture to bind and enrich low abundance proteins, while excluding unwanted high abundance proteins, resulting in significantly improved proteome coverage.

This workshop will provide an overview of the Nanotrap® particle technology, including methods and applications. A case study also will be presented, wherein the Nanotrap® particle technology coupled with mass spectrometry was used to confirm an age-related macular degeneration biomarker identified using the SOMAscan® platform. The increased plasma protein coverage enabled by the Nanotrap® particle / mass spectrometry-based approach identified over 100 additional proteins that exhibited more significant changes between disease state and the controls then the previously identified biomarker.

PerkinElmer
@ Rm3

PerkinElmer’s Comprehensive Portfolio for Automated Sample Preparation and Mass Spectrometry Analysis

Please join us to discuss workflows and applications for GC/MS, ICP/MS, and LC/MS/MS. Examples will include automated liquid handling for sample preparation, sensitive and robust detection, and advanced data analysis across PerkinElmer’s portfolio of mass spectrometry equipment.

Moderated by Aaron Risinger, MBA
Sr. Mass Spectrometry Sales Specialist
PerkinElmer Diagnostics

MSACL
@ Rm4

The Status of Clinical Mass Spectrometry in China
Chair: Michael Chen

1. Development and Prospect of Mass Spectrometry in Clinical Laboratory of China
Chengbin Wang (Chinese PLA General Hospital)


2. Development of Clinical MS in the University Setting : LC-MS/MS-based Clinical Hormone Detection
Wei Guo (Fudan University)


3. Role of Independent Clinical Laboratories in Promoting Clinical Mass Spectrometry in China
Zhouyang Kang (Hangzhou Calibra Diagnostics)


4. Advancing Mass Spectrometry-based Technologies for Clinical Applications
Keqi (Jacky) Tang (Ningbo University)

Thermo Fisher Scientific
@ Rm5

Title: Ease-of-Use without compromise in clinical LC-MS/MS

Speakers: Peter Cooke, Regional Marketing Manager, Thermo Fisher Scientific
Marta Kozak, Lead Scientist, R&D, Thermo Fisher Scientific

Overview of the workshop:
Get first-hand reports on the quality and reliability of the Cascadion™ 25-Hydroxy Vitamin D assay* results from Europe and the easy-to-use features of the fully automated Cascadion™ SM Clinical Analyzer.* Hear from an industry expert, a clinical LC-MS/MS subject matter specialist trained on the Cascadion system, and operators from European clinical beta trial sites.

Learning objectives:
− Discover how LC-MS/MS technology can be easy to implement in clinical labs today
− Appreciate the quality of the data from the Cascadion SM Clinical Analyzer, through an overview of the European Cascadion SM 25-Hydroxy Vitamin D Assay beta trials
− Learn how the features of the Cascadion analyzer translate to performance as seen through clinical laboratory experiences in Europe

*Product is not 510(k) cleared and is not yet available for sale in the U.S.

845
900
Intermission
@ Ballroom Foyer
900
1000
Exhibitor Feedback Meeting
@ Mesquite H

Scientific Session 5

Track 1
Pueblo

MS Imaging in Clinical Applications
Chair: Jeff Spraggins

Track 2
Room 1

Practical Training : How Programming Will Save You Time
Chair: Shannon Ellis

Track 3
Room 2

Small Molecule Hot Topics: It's Not Monkey Business
Chair: Joshua Hayden

Track 4
Room 3

Lipidomics in Disease Diagnostics
Chair: Anne Bendt

Track 5
Room 4

Using Software to Improve LC-MSMS Assay Output
Chair: Irene Van den Broek

Track 6
Room 5

MS and Microbes: It’s More Than MALDI
Chair: Chris Cox

900
920
Desorption Electrospray Ionization Mass Spectrometry as a Tool for Diagnosis of Thyroid Nodules from Fine Needle Aspiration Biopsies
Rachel DeHoog
University of Texas at Austin
Making a Case for Why Programming Will Save You Time
Shannon Ellis
University of California San Diego
Development of an LC-MS/MS Method for Assessment of Mutant Isocitrate Dehydrogenase (mIDH) Cancer Biomarkers in Acute Myeloid Leukemia
Jeffrey Whitman
University of California, San Francisco
Investigating Smooth Muscle Foam Cells in Atherosclerosis – Applications of Tandem Mass Spectrometry
Joshua Dubland
University of British Columbia
The Skyline Software Ecosystem: Efforts to Achieve Transparency, Interoperability, and Robustness of Quantitative LC-MS Assays
Michael MacCoss
University of Washington
Metabolomics of Oxalobacter Formigenes to Characterize Intestinal Oxalate Secretion
Casey Chamberlain
University of Florida
920
940
Intraoperative-MS-based Biopsy Analysis Platform for Glioma Diagnosis, Extent of Tumor Cell Infiltration Estimation, and IDH Subtyping
Clint Alfaro
Purdue University
View Slides (PDF)

...
Extended Session
...
Mass Spectrometry and Mass-Overdose in New Haven, Connecticut
Dustin Bunch
Yale-New Haven Hospital
Sterol Profile Analysis for Cholesterol Biosynthesis Disorders by Label-Free LC-MS/MS
Matthew Nichols
Post- Doctoral Fellow
...
Keynote
...
Analytical Strategies to Study the Interaction Between Microbiota and Drug Metabolism using Targeted and Untargeted LC-MS-based Metabolomics
Marine Letertre
Imperial College London
940
1000
Rapid Evaporative Ionization Mass Spectrometry Explores Tumor Necrosis and Tissue Autolysis
Pierre-Maxence Vaysse
Maastricht University
...
Extended Session
...
Analysis of Steroid Hormones in Serum and Follicular Fluid of Female Rhesus Macaques with Chronic Combined Hyperandrogenemia and/or Western-Style Diet
David Erikson
Oregon National Primate Research Center
Lipidomic Profiling of Extracellular Vesicles Released by Breast Cancer Cells and its Potential for the Identification of Biomarkers for Breast Cancer Diagnosis.
Erika Dorado
Imperial College London
...
Keynote
...
Developing Laser Assisted Rapid Evaporative Ionization Mass Spectrometry (LA-REIMS) as an Automated High-Throughput Platform for Clinical Microbiology
Alvaro Perdones-Montero
Imperial College London
1000
1100
Exhibits & Poster Session
@ Exhibit Hall in Oasis 3-4

Positions C to be attended.
1015
1100
Meet-a-Mentor: Poster Tours 2
@ Exhibit Hall in Oasis 3-4

Join a mentor as they tour the posters.

Scientific Session 6

Track 1
Pueblo

MS Imaging to Investigate Disease Behavior
Chair: Livia S. Eberlin

Track 2
Room 1

Practical Training : Everything You Wanted to Know About Internal Standards But Were Too Afraid to Ask
Chair: Russell P. Grant

Track 3
Room 2

Natural Products
Chair: Margret Thorsteinsdottir

Track 4
Room 3

Keynote: Translation of Lipidomic Technologies Towards Quantification of Blood Lipids
Chair: David Herold

Track 5
Room 4

New Proteomic Assays
Chair: Mari DeMarco

Track 6
Room 5

Machine Learning
Chair: TBA

1100
1120
Spatial Metabolomics Identifies a Glomerular Sphingomyelin as a Potential Therapeutic Target for the Treatment of Diabetic Kidney Disease
Guanshi Zhang
UT Health San Antonio
Everything You Wanted to Know about Internal Standards But Were Too Afraid to Ask
Russ Grant
Labcorp
View Slides (PDF)

Clinical Evaluation of Interaction of Phytoestrogenic Botanical Dietary Supplements with Drug Metabolism in Women
Luying Chen
Linus Pauling Institute, Oregon State University
Translation of Lipidomic Technologies Towards Quantification of Blood Lipids
Markus Wenk
National University of Singapore
Development of a Fit-For-Purpose LC-MRM-MS Assay to Measure Prion Protein in Cerebrospinal Fluid
Eric Kuhn
Broad Institute
Automating HPLC Peak Detection using Convolutional Neural Networks
Matthew Boutell
Rose-Hulman Institute of Technology
1120
1140
Predicting an Early Response of Tissue to Pharmacological Treatment Prior to Changes in Histology
Katy Margulis
Stanford University
...
Extended Session
...
Hormone Modulations of 4-Hydroxyestradiol, 5α-Androstane-3α,17β-diol, and Glucocorticoids in Urine of Menopausal Women Taking a Hop Botanical Dietary Supplemen
Alan Wong
Linus Pauling Institute, Oregon State University
...
Keynote
...
A LC-MS/MS Method using Antibody Free Sample Preparation for Multiplexed Targeted Quantitative Intact Protein Biomarkers of Diabetes from Human Serum
Jesse Seegmiller
University of Minnesota
High-Throughput Metabolomics Combined with Artificial Intelligence for Prognostic and Diagnostic Assessments
Jacques Corbeil
Laval University
1140
1200
Imaging Mass Spectrometry Reveals Crosstalk Between the Fallopian Tube and the Ovary that Drives Primary Metastasis of Ovarian Cancer
Laura Sanchez
University of Illinois at Chicago
...
Extended Session
...
Study on the Association between Hypercholesterolemia and High Phytosterol Levels in Children via Utilization of a Triplex Phytosterol GC-MS Assay
Joon Hee Lee
Seoul National University Hospital
...
Keynote
...
Early Prediction of Cardiac Events via Remote Monitoring Coupled with an Apolipoprotein Targeted MRM Panel
Kelly Mouapi
Cedar-Sinai Medical Center
Open Access Data: MIMIC-III, eiCU
Christina Chen
Beth Israel Deaconess Medical Center, HMS, MIT
1200
1300
Clinical MS in Asia Open Forum
@ Exhibit Hall in Oasis 3-4

Meet at Booth #42-44

Meet key players from the US and Asia working to develop clinical mass spectrometry in Asia.

1200
1300
Lunch
@ Exhibit Hall in Oasis 3-4

Scientific Session 7

Track 1
Pueblo

Multiplexing in Clinical Application, Translation and New Frontiers
Chair: Daniel Holmes

Track 2
Room 1

Practical Training : Potential to Improve Patient Care : Therapeutic Drug Monitoring (TDM) using LC-MS/MS
Chair: Paul Jannetto

Track 3
Room 2

Drug Analysis: Monitoring Efficacy and Detecting Exposure
Chair: Angela Fung

Track 4
Room 3

Metabolomics & Imaging
Chair: Rachel DeHoog

Track 5
Room 4

Neuroproteomics
Chair: Andrew Hoofnagle

Track 6
Room 5

Applied Informatics
Chair: Alec Saitman

1300
1320
Simultaneous Glycopeptide Profiling of Immunoglobulin G and A Reveals Glycan Changes in Colorectal Cancer
Ana Momčilović
Leiden University Medical Centre
Applications of Mass Spectrometry for Therapeutic Drug Monitoring (TDM)
Paul Jannetto
Mayo Clinic
Tape Stripping and High Performance LC-MS/MS Measuring Chlorhexidine Gluconate on Skin
Xueheng Zhao
Cincinnati Children's Hospital Medical Center
Assessing the Effect of Nitisinone Induced Hypertyrosinaemia on Monoamine Neurotransmitters in a Murine Model of Alkaptonuria using Mass Spectrometry Imaging
Andrew Davison
University of Liverpool
A Comprehensive Map and Functional Annotation of the Normal Human Cerebrospinal Fluid Proteome
Yajie Wang
Dept of Clinical Laboratory, Beijing Ditan Hosp
No Typing Allowed: Reflections and Advice One Year After Automating All Mass Spectrometry Result Entry at a Regional Clinical Reference Laboratory
Benjamin Beppler
TriCore Reference Laboratories
1320
1340
Immunoassays in Multiplex for Biomarker Discovery and Validation
Thomas O. Dr. Joos
NMI at the University of Tuebingen
Making the Switch: Issues & Challenges to Adopt Mass Spectrometry for Therapeutic Drug Monitoring (TDM)
William Clarke
Johns Hopkins School of Medicine
Quantification of Superwarfarin Rodenticides in Plasma using High-Performance LC-MS/MS
Daniel Nosal
Oregon State University - Linus Pauling Institute
Ambient Ionization Mass Spectrometry Imaging for Prognostic Metabolic Signatures in Clear Cell Renal Carcinoma (ccRCC)
Vijaya Lakshmi Kanchustambham
Stanford University
Screening for Alzheimer’s Disease: Summary and Future Directions of an Amyloid-Beta Blood Test
Melissa Budelier
Washington University in St Louis
Clinical Mass Spectrometry Workflow Improvements by Transitioning from Manual to Auto-Data Review
Alec Saitman
Providence Health and Services
1340
1400
Toxicology Screening by Mass Spectrometry for Emergency Intoxication
Shuijun Li
Shanghai Xuhui Central Hospital, China
...
Extended Session
...
Concordance of Paired Maternal and Newborn Urine Drug Screens using Low Cutoffs for Positivity
Stephen Roper
Washington University School of Medicine
Spatially Resolved Analysis of Thermally Injured Skin using Desorption Electrospray Ionisation Mass Spectrometry Imaging
Josh Cuddihy
Imperial College London, UK
TDP-43 Enrichment using a High Affinity RNA Aptamer
Taylor Pobran
University of British Columbia
Monitoring Clinical Mass Spectrometry System Performance
Shannon Haymond
Northwestern University
1400
1530
Exhibits & Poster Session
@ Exhibit Hall in Oasis 3-4

Positions D to be attended from 14:15-15:15. Poster Finalists to attend their posters for final interviews.
1430
1530
CMS Journal Open Forum
@ Exhibit Hall in Oasis 3-4

Meet at Booth #42-44

A toast to the Clinical Mass Spectrometry journal reaching 50 publications and filing for PubMED listing and Impact Factor.

1515
1530
Remove Posters
@ Exhibit Hall in Oasis 3-4

You can store your poster between Smoketree F and Rm6 until 20:00.
1515
1630
Plenary Reception
@ Ballroom Foyer
1530
Exhibits Closed
@ Exhibit Hall in Oasis 3-4
1530

Plenary Lecture


@ Rooms 3-4

1530
1615
Tools and Infrastructure to Enable Crowdsourcing the Molecular Analysis of Microbiome as Applied to Precision Care
Pieter Dorrestein
University of California, San Diego

The general community is encouraged to visit the same primary care physician. This is because they

1615
1630
Intermission
@ Ballroom Foyer
1630
1800

FDA Viewpoint
FDA Overview of the Process for Clearance and Approval of Mass Spectrometry-based In vitro Diagnostic Devices with Doug Jeffery, Majda Haznadar & Kristian Roth.

FDA Overview of the Process for Clearance and Approval of Mass Spectrometry-based In vitro Diagnostic Devices
@ Room 3-4

Chair(s): Andy Hoofnagle

Panelists:
Doug Jeffery, Majda Haznadar & Kristian Roth

FDA recognizes the importance of outreach to facilitate successful clearance or approval of future pre-market submissions. Staff from the Center for Devices and Radiological Health, Office of In Vitro Diagnostics and Radiological Health, are back at MSACL 2019 to discuss ways to interact with the FDA early through the pre-submission program to facilitate successful pre-market submissions. We will also discuss different types of pre-market submissions, namely 510(k)s and De Novos. This year, FDA will also discuss the benefits of MALDI-TOF MS to the clinical laboratory and how regulatory review has advised the medical device industry on study designs to support regulatory clearance from analytical through clinical trials to market. In this portion of the session, FDA will provide a historical overview of the cleared MALDI-TOF MS devices and highlights of the newly created exemption for these devices. The discussion will explain the intent of the proposed exemption and how it will be applied.

1800
1815
Poster Awards
@ Rooms 3-4

Join us in congratulating the winners of the poster contest.
1815
1830
Final Remarks
@ Rooms 3-4

The Scientific Committee Chair and Vice Chair wrap up the week and share information about upcoming Conferences.
1830
2000
Closing Dinner
@ Poolside

A final seated meal and opportunity to relax into the evening. After your meal, you may choose to wander downtown to explore Village Fest - http://villagefest.org/.
1930
2100
Hospitality Lounge
@ Rocks Patio
2200
Conference Closed
@ Ballroom Foyer

Friday

830
1000
SciCom Meeting
@ San Jacinto

A private meeting for Scientific Committee members to shape the vision and requirements for a consistent, high-quality MSACL 2020 Scientific Program. Breakfast will be served.

1. Establish topics of particular interest to highlight in 2020, allowing SciCom members ample time to solicit and formulate programming. Of particular interest is science that appeals to multiple topic areas, and can encourage communication between disciplines.
3. Begin nominations for 2020 Keynotes/Plenaries.
4. Confirm 2020 Committee members.