Accelerating the Implementation of Mass Spectrometry in the Clinical Lab

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UNITED STATES 2019

Help Us Reach Our Educational Support Goal of $40,000
Educational Travel Grants supported in part by:
Brian Kelly, Amadeo Pesce, Danyel Tacker
&

Short Courses

MSACL hosts a diverse offering of Short Courses.

Short Courses will occur over the first three days of MSACL (Sunday, Monday, Tuesday of March 31 - April 2).

Courses are NOT replicated on different days. They are single courses that span 1 or 2 days.

Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Clinical MS Review
Clinical MS 301
A Comprehensive Review of Clinical Mass Spectrometry Technology & Techniques, including Miniaturization
Jack Henion, PhD
Location: TBA
Group: TBA
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00
Data Science
Data Science 101
Breaking up with Excel: An Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Stephen Master, MD, PhD
Location: TBA
Group: TBA
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00
Data Science 201
Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling
Patrick Mathias, MD, PhD & Randall Julian, PhD
Location: TBA
Group: TBA
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00
LC-MS
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Lorin Bachmann, PhD & Grace van der Gugten
Location: TBA
Group: TBA
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00
LC-MSMS 201
Understanding and Optimization of LC-MS/MS to Develop Successful Methods for Identification and Quantitation in Complex Matrices
Robert D. Voyksner, PhD
Location: TBA
Group: TBA
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD, Erik J. Soderblom, PhD
Location: TBA
Group: TBA
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00
LC-MSMS 301
Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics
Brian Rappold, Matthew Crawford and Chris Shuford
Location: TBA
Group: TBA
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00
MALDI
MALDI 103
MALDI-MS Fundamentals and its Emerging Role in Pathology and Laboratory Medicine
Mark W. Duncan, PhD & Mari L. DeMarco, PhD
Location: TBA
Group: TBA
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00
Metabolomics
Metabolomics 201
Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis
Timothy Garrett, PhD & Erin Baker, PhD
Location: TBA
Group: TBA
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00
Proteomics
Proteomics 201
Clinical Proteomics
Andy Hoofnagle, MD, PhD, Cory Bystrom, PhD & Chris Shuford, PhD
Location: TBA
Group: TBA
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00
Sample Preparation
Sample Prep 201
Sample Preparation and Alternative Matrices for LC-MS Assays
William Clarke, PhD & Mark Marzinke, PhD
Location: TBA
Group: TBA
Not
in
Session
STARTS <
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00
Toxicology
Forensic Toxicology 101
Basic Forensic Toxicology
Jarrad Wagner, Ph.D., F-ABFT, Allison Veitenheimer, Ph.D., Russell Lewis, Ph.D., F-ABFT & Robert Johnson, PhD, F-ABFT
Location: TBA
Group: B
STARTS <
Sunday
14:00-18:00
> ENDS
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Not
in
Session
Not
in
Session
Forensic Toxicology 201
Advanced Forensic Toxicology
Jarrad Wagner, Ph.D., F-ABFT, Allison Veitenheimer, Ph.D., Russell Lewis, Ph.D., F-ABFT & Robert Johnson, PhD, F-ABFT
Location: TBA
Group: B
Not
in
Session
Not
in
Session
Lunch
Monday
12:00-13:00
STARTS <
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00


Clinical MS 301 :: A Comprehensive Review of Clinical Mass Spectrometry Technology & Techniques, including Miniaturization

Level:2-3 (Intermediate - Advanced)
Prereqs:Students who sign up for this advanced course should either have five years or more of personal LC/MS/MS experience and familiarity with the scientific literature or have taken one or more introductory courses which cover atmospheric pressure ionization (API) techniques as well as the basics involved in routine LC/MS and LC/MS/MS analyses.
Location:TBA
Group:TBA
Instructor(s):Jack Henion, PhD

Jack Henion is Professor Emeritus of Toxicology at Cornell University in the Analytical Toxicology Section of the Diagnostic Laboratory within the College of Veterinary Medicine. He is also a co-founder, Chairman and CSO of Advion BioSciences (ABS), Inc., located in Ithaca, New York. Dr. Henion's work in LC/MS relates to the analysis of real-world samples common to pharmaceutical, environmental, and biochemical problems. The instructor has published extensively in the areas of conventional capillary GC/MS as well as LC/MS, SFC/MS, IC/MS, and CE/MS using atmospheric pressure ionization (API) technologies with quadrupole, ion trap, and time-of-flight mass spectrometers.


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00

Overview: This course presents presents a comprehensive overview of technology and techniques of analytical mass spectrometry and from that foundation extends into exciting, disruptive recent developments.

  1. Sample preparation
    • Topics: New types of extraction, Issues to consider, Isolation of proteins from biological samples Ultrafiltration, Affinity techniques, Molecularly Imprinted Polymers (MIP?s), Aptamers, Thermo's MSIA pipette tips, Electro Extraction, Quechers, SISCAPA, Micro extractions: Dried blood spots (DBS), Dried Plasma Spots (DPS).
  2. Advanced separation techniques for large molecules
    • Topics: UHPLC, Hydrophobic Interaction Chromatography (HIC), Nano-UHPLC, Micro LC/MS, Size Exclusion Chromatography (SEC), ion exchange chromatography, Capillary Electrophoresis (CE), Differential Mobility Spectrometry (DMS).
  3. Ionization techniques for MS
    • Topics: Electrospray ionization (ESI), Nano ESI, Micro ESI, Atmospheric pressure chemical ionization (APCI), Atmospheric pressure photoionization (APPI), Matrix assisted laser desorption ionization (MALDI), LAESI, Electron Ionization (EI) and its potential for LC/MS.
    • To Discuss: New ionization techniques which may be used without on-line separation science technology. This area has evolved into a variety of ambient ionization techniques such as DESI, DART, ASAP, etc.
  4. Mass Analyzers
    • Quadrupoles, Ion traps, linear and quadrupole, Time-of-Flight (TOF), Orbitraps, Hybrid mass analyzer systems, Ion mobility spectrometers, and Differential Mobility Spectrometry (DMS).
    • To Discuss: Developments and improvements in mass analyzers including linear ion traps, FTMS, time-of-flight (TOF), orbitraps, and accelerator mass spectrometry (AMS), the latter currently being applied to micro-dosing experiments by the pharmaceutical industry. Issues such as full-scan acquisition rates, high-resolution mass spectrometry (HRMS), the importance and usefulness of exact mass measurements for qualitative and quantitative analysis, and the analytical merits compared with modern SRM LC/MS experiments will be discussed with many practical examples and applications. The latter will include clinical chemistry issues as well as pharmaceutical, food safety, environmental and industrial examples.
  5. Imaging and profiling by MS
    • Applications of recently reported ionization techniques for imaging the location of chemicals in various matrices employing MALDI, DESI, LAESI, LESA and other techniques.
    • Topics: The technique of MALDI and its applications to tissue imaging as well as DESI, LAESI and also liquid extraction surface analysis (LESA) employing nano-electrospray. A comparison of the various classes of compounds where MALDI and nano ESI provide complimentary coverage of certain compounds found in biological and other matrices.
  6. High resolution MS
    • Topics: Fundamentals, Mass Defects, Isotopic patterns, Mass axis calibration, Types of HRMS systems, Qual/Quan Analysis, Data mining processes, Future directions
    • To Discuss: The analytical merits of HRAMS from QTOF as well as orbitraps and FTMS systems will be presented. Instances where either SRM LC/MS or LC HRAMS may be preferred for optimal selectivity due to chemical background or other interference issues.
  7. Miniaturization in MS
    • Topics: Purdue University "Mini 11", Torion, Microsaic, Advion expression CMS, Waters QDa
    • To Discuss: The benefits and limitations of smaller analytical instrumentation systems will be compared. This includes miniaturization of HPLC systems as well as the mass spectrometers themselves. The commercial introduction of chip-based HPLC systems closely integrated with mass spectrometers offers a glimpse of future directions in analytical chemistry.
  8. Synergistic Integration
    • A systematic overview via specific examples with applications highlighting noted examples of innovative novel and non-standard technologies which demonstrate the analytical potential of new analytical technologies.
    • Developing instrumentation and technologies will be important aspects of future mass spectrometry techniques and its expansion to important new applications. An extremely important example is the need for LC/MS bioanalysis (quantitation) of biologics (ADC?s, large molecules, RNA, etc.) in biological samples employing both bottom up and top down methods. HRAMS coupled with „protein friendly? chromatography will significantly expand our present analytical capabilities. Ion mobility spectrometry (IMS) and transportable mass spectrometers could lead to point-of-care applications and other far reaching applications of mass spectrometry beyond what we are doing today. The future is very exciting!

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$80$96$135
Academic / Non-Profit$240$288$405
Commercial / Industry$400$480$670
*Short Course Registration is separate from Conference Registration.



Data Science 101 :: Breaking up with Excel: An Introduction to the R Statistical Programming Language

Level:1-2 (Beginner - Intermediate)
Prereqs:- knowledge of Excel
- able to bring a laptop
- able to pre-install software on their laptop...Namely: R and R-studio
- willingness to break up with Excel
Location:TBA
Group:TBA
Instructor(s):Daniel Holmes, MD & Stephen Master, MD, PhD

Co-Instructor: Daniel Holmes, MD

Clinical Associate Professor, Department of Pathology and Laboratory Medicine, University of British Columbia

Daniel Holmes did his undergraduate degree in Chemical Physics from the University of Toronto with a focus on Quantum Mechanics. He went to medical school at the University of British Columbia (UBC) where he also did his residency in Medical Biochemistry. He is a Clinical Associate Professor of Pathology and Laboratory Medicine at UBC and Division Head of Clinical Chemistry at St. Paul's Hospital in Vancouver. Interests include laboratory medicine statistics, clinical endocrinology, clinical lipidology and clinical mass spectrometry. Assay development efforts in the last two years have focused on assays specialized endocrine testing.

Co-Instructor: Stephen Master, MD PhD

Associate Professor of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia

Stephen Master received his undergraduate degree in Molecular Biology from Princeton University, and subsequently obtained his MD and PhD from the University of Pennsylvania School of Medicine. After residency in Clinical Pathology at Penn, he stayed on as a faculty member with a research focus in mass spectrometry-based proteomics as well as extensive course development experience in bioinformatics. He is currently Associate Professor of Pathology and Laboratory Medicine at Children’s Hospital of Philadelphia. One of his current interests is in the applications of bioinformatics and machine learning for the development of clinical laboratory assays. He would play with R for fun even if he weren't getting paid, but he would appreciate it if you didn't tell that to his department chair.


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00

Overview: Have you ever tried to do Deming regression in Excel only to discover that it is not available? Have you had your figure rejected by a journal because the resolution was not good enough? Have you wished that you could figure out a way to stop manually transcribing your LC-MS/MS results into the LIS?

Well, your wait is over because this year at MSACL we will be offering a course for complete programming newbies that will help you get going analyzing real data related to LC-MS/MS assay development, validation, implementation and publication. The only background expected is the ability to use a spreadsheet program. The skills that you will acquire will allow you to take advantage of the many tools already available in the R language and thereafter, when you see that your spreadsheet program does not have the capabilities to do what you need, you will no longer have to burst into tears. You will be empowe-R-ed.

The course will be run over two days and time will be evenly split between didactic sessions and hands on problem solving with real data sets. Drs Holmes and Master will adopt a “no student left behind policy”. Students will be given ample time to solve mini problems taken from real life laboratory work and focused on common laboratory tasks. All attendees will need to bring a laptop with the R language installed R Studio interface installed. Students may use Windows, Mac OSX or Linux environments. Both R and R studio are free and open-source. No cash required.

Students should be prepared for learning what computer programming is really like. This may involve some personal frustration but it will be worth it.

Obtaining the Software

Instructions for installing the R language are here: http://cran.r-project.org/
Instructions for installing R Studio are here: http://www.rstudio.com/

Course Description

The course will cover:

  1. The major types of R variables: vectors (numerical, character, logical), matrices, data frames and lists.
  2. The important classes: numeric, character, list and changing between them
  3. Importing data from Excel
  4. Dealing with non-numeric instrument data: the “<10”s and “>1000”s.
  5. Manipulating your data: sub-setting, which, match, sort, unique, cut
  6. Simple statistical tests: mean, median, quantiles (normal ranges), t-tests, ANOVA, Wilcoxon.
  7. Data merges: matching rows between sets
  8. Exporting data to Excel-like format.
  9. Regressions: Ordinary Least Squares,Passing Bablok, Deming, weighted regressions.
  10. Non-linear regressions
  11. Looping: Doing things repeatedly
  12. Writing your own functions
  13. Making highly customized graphs: scatter plots, regression lines, histograms, box plots, qq-plots
  14. Putting it all together projects:
    • Preparing method comparison regression and Bland Altman plots
    • Preparing mass spectrometry data for upload to LIS.

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$80$96$135
Academic / Non-Profit$240$288$405
Commercial / Industry$400$480$670
*Short Course Registration is separate from Conference Registration.



Data Science 201 :: Going Further With R: Tackling Clinical Laboratory Data Manipulation and Modeling

Level:Intermediate
Prereqs:Completed “Breaking Up With Excel” and/or familiar with basic R concepts.
Able to bring a laptop.
Able to pre-install software on laptop...Namely: R and R-studio.
Location:TBA
Group:TBA
Instructor(s):Patrick Mathias, MD, PhD & Randall Julian, PhD

Co-Instructor: Patrick Mathias, PhD

Assistant Professor, Department of Laboratory Medicine, University of Washington

Patrick Mathias completed his undergraduate degree in Electrical Engineering at Duke University, followed by a master’s degree in Electrical and Computer Engineering from the University of Illinois at Urbana-Champaign. He then completed a MD degree and a PhD in Bioengineering from the University of Illinois, with a focus on nanophotonics and label-free biosensors. He completed residency training in Clinical Pathology as well as a Clinical Informatics fellowship at the University of Washington. He is currently the Associate Director of the Informatics division in the Department of Laboratory Medicine at the University of Washington. His clinical and research interests lie in improving electronic health record systems to improve ordering and interpretation of laboratory tests, developing infrastructure for novel analytical technologies in the clinical laboratory, and applying analytics to improve laboratory operations and clinical care at a population level.

Co-Instructor: Randy Julian, PhD

CEO, Indigo BioAutomation

Randy Julian is Founder and CEO of Indigo BioAutomation located in Indianapolis, Indiana. Randy earned a Ph.D. in Chemistry from Purdue University in 1993 and then worked in Discovery Chemistry at Eli Lilly for 14 years. At Lilly Dr. Julian worked on natural product discovery, high throughput screening for RNA anti-viral compounds and researched methods for using proteomics to optimize drug candidates in animal models. Randy founded Indigo based on informatics technology developed during his time with Lilly. Indigo now provides laboratory data analysis software which uses machine intelligence to automatically analyze over 100 million sample results per month for every major clinical laboratory in the US.

Dr. Julian is an active member of the clinical mass spectrometry community, teaches short courses in statistics, informatics and analytics. Randy is the past Chairman of the Human Proteome Organization’s Standards Initiative. He is the coauthor of two international standards for analytical data. He was also the chairman of the ASTM committee on mass spectrometry data standards. Dr. Julian maintains an active research relationship with the faculty at Purdue University where he is an Adjunct Professor of Chemistry.


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00

Overview: Having completed your first steps into the wonderful world of data analysis with R, would you like to go further? You’ve learned the basics of R, so now it’s time to put that knowledge to work and tackle some interesting clinical applications. Along the way you will also be introduced to even more of capabilities of R and the tools developed by the amazing R community.

The course will be run over two days and time will be split between lecture sessions, individual problem solving, and a highly interactive group-level data mining of real data sets (there may even be prizes). Like the introductory course, this class will maintain the “no student left behind policy”. Students will be given time to solve problems taken from real life laboratory work and to do some more advanced analysis on large scale data sets. All attendees will need to bring a laptop with the R language installed and R Studio interface installed. Students may use Windows, Mac OSX or Linux environments. Both R and R studio are free (as in “Free Beer”) and open-source.

Students should be prepared continue to expand their skill in programming – which, as you learned in the introductory course can be a little frustrating, but not as frustrating as not being able to get the computer to do what you want at all!

Obtaining the Software

Instructions for installing the R language are here: http://cran.r-project.org/
Instructions for installing R Studio are here: http://www.rstudio.com/

Course Description

The course will cover:

  1. Using the “Tidyverse”: a powerful collection of tools for working with R
  2. Conceptual basis for keeping data “tidy”
  3. Using the Import -> Tidy -> Transform -> Visualize -> Model -> Communicate pipeline
  4. Parsing non-tabular data formats such as XML and JSON
  5. Importing data from various sources including databases and web scraping
  6. Data wrangling and tools for cleaning up data before attempting anything
  7. Data visualization with the ggplot2 library
  8. Functional programming concepts for efficient iteration (purrr’s map functions)
  9. Fitting models which contain numeric and non-numeric data
  10. Introduction to R-Markdown for report generation
  11. Looping: Doing things repeatedly
  12. Writing your own functions
  13. Making highly customized graphs: scatter plots, regression lines, histograms, box plots, qq-plots
  14. Putting it all together projects:
    • Preparing method comparison regression and Bland Altman plots
    • Preparing mass spectrometry data for upload to LIS.

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$80$96$135
Academic / Non-Profit$240$288$405
Commercial / Industry$400$480$670
*Short Course Registration is separate from Conference Registration.



LC-MSMS 101 :: Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory

Level:1-2 (Beginner - Intermediate)
Prereqs:
Location:TBA
Group:TBA
Instructor(s):Lorin Bachmann, PhD & Grace van der Gugten

Co-Instructor: Lorin Bachmann, PhD, DABCC

Lorin is Associate Professor of Pathology and Co-Director of Chemistry at Virginia Commonwealth University Medical Center. Dr. Bachmann’s areas of interest include implementation of LCMS in the clinical laboratory and laboratory test standardization. She routinely contributes to development of laboratory practice guidelines and has provided many lectures on practical implementation of clinical mass spectrometry.

Co-Instructor: Grace Van Der Gugten

Grace is LC-MS/MS Applications Development Specialist at St Paul’s Hospital in Vancouver BC. She is passionate about developing the most user friendly and streamlined LC-MS/MS assays as possible for routine use in the Special Chemistry Mass Spec Lab. She loves troubleshooting, especially when the cause of problem has been discovered and the issue solved!


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00

Overview: Is your laboratory under pressure to purchase an LC-tandem MS or is the ROI you wrote last year haunting you now? This short course is designed for attendees implementing quantitative LC-tandem MS for patient testing who have laboratory medicine experience but no mass spectrometry training - CLS bench analysts, supervisors, R&D scientists, and laboratory directors. Theoretical concepts necessary for a robust implementation of clinical mass spectrometry will be presented – but the emphasis is on practical recommendations for:

  1. LC-MS/MS system purchasing
  2. site preparation and installation
  3. defining preliminary MSMS and LC parameters for your first method
  4. selecting and optimizing sample preparation for your first method
  5. choosing internal standards, solvents, and water, making reagents and calibrators
  6. adjusting sample preparation, LC and MSMS parameters to achieve the desired assay performance
  7. establishing data analysis & review criteria and an interface to the LIS
  8. pre-validation stress testing and method validation
  9. preventative maintenance and troubleshooting
  10. maintaining quality in production.

Our goal is to present just enough theory so you can report high quality results, while opening a window to the depth and complexity of clinical mass spectrometry such that your appetite is whetted to learn more.

Previous exposure to the principles of clinical method validation, either didactic or practical, is assumed. A glossary of common LC-MSMS terms/acronyms, and diagrams delineating basic LC and MSMS instrument components and functions will be emailed to attendees a week prior to the beginning of the course. This material will also be addressed at the beginning of the course, but the initial learning curve can be steep and review prior to the course will be beneficial if you have absolutely no previous exposure with LC-MSMS.

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$80$96$135
Academic / Non-Profit$240$288$405
Commercial / Industry$400$480$670
*Short Course Registration is separate from Conference Registration.



LC-MSMS 201 :: Understanding and Optimization of LC-MS/MS to Develop Successful Methods for Identification and Quantitation in Complex Matrices

Level:2 (Intermediate)
Prereqs:General knowledge of laboratory techniques associated with HPLC and mass spectrometry and some hands-on experience with running an LC/MS system.
Location:TBA
Group:TBA
Instructor(s):Robert D. Voyksner, PhD

Instructor: Robert D. Voyksner, PhD

Dr. Robert D. Voyksner received his B.S. in Chemistry at Canisius College in 1978 and his Ph.D. at the University of North Carolina at Chapel Hill in 1982. He was employed at Research Triangle Institute (RTI) from 1983-2001 as the director of the mass spectrometry facility and has been responsible for developing extraction, separation and mass spectrometric methods for biologically and environmentally significant compounds. His work earned him the Presidents Award, the highest award within RTI. In 2001 he co-founded LCMS Limited in Raleigh, NC and has been the President of the company to date. Under his direction LCMS Limited is working on technological advancements in LC/MS, offering services to pharmaceutical, clinical and agrochemical industry for solving unique problems by LC/MS/MS and offering training in LC/MS/MS and MS/MS interpretation and on LC/MS/MS instrumentation. Dr Voyksner is also an Adjunct professor at the North Carolina a School of Vetinary Medicine and at The University of North Carolina School of Pharmacy.

Dr. Voyksner's research in mass spectrometry has resulted in over 230 publications and presentations, primarily in the area of HPLC/MS. He has served on the Board of Directors for The American Society For Mass Spectrometry (ASMS), is on the organization committee for The Montreux LC/MS Symposium and was the organizer for the 1995, 1999, 2003 and 2007 Montreux LC/MS Symposia. Dr. Voyksner has taught over 100 courses on LC/MS, CE/MS and CID interpretation during the past 10 years for ASMS, pharmaceutical companies; ISSX, PBA, HPCE and HPLC focused meetings.


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00

Overview: This course is designed for the chromatographer / mass spectrometrist who want to be successful in developing methods, method optimization and solving problems using LC/MS/MS. The course covers the atmospheric pressure ionization (API) techniques of electrospray, pneumatically assisted electrospray and atmospheric pressure chemical ionization (APCI) and atmospheric pressure photo ionization (APPI) using single quadrupole, triple quadrupole, time-of-flight and ion trap mass analyzers.

Discussions of sample preparation and chromatography will target method development and optimization for the analysis of "real-world" samples by LC/MS/MS.

The course highlights the following topics with respect to optimization a method to achieve the best sensitivity, specificity and sample throughput:

  1. Optimization ionization in API techniques,
  2. understanding and minimizing matrix suppression,
  3. relative merits of various LC column lengths, particle sizes and column diameters for LC/MS/MS analysis,
  4. introduction into the interpretation of MS/MS spectra,
  5. important issues in LC/MS/MS quantitation, and
  6. optimization of an quantitative analysis.

Applications of LC/MS/MS to analyze compounds of clinical interest in biological matrices will be discussed throughout the course to emphasize the topics covered.

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$80$96$135
Academic / Non-Profit$240$288$405
Commercial / Industry$400$480$670
*Short Course Registration is separate from Conference Registration.



LC-MSMS 202 :: Practical LC-MS Maintenance and Troubleshooting

Level:2 (Intermediate)
Prereqs:Experience with LC-MS systems.
Location:TBA
Group:TBA
Instructor(s):J. Will Thompson, PhD, Erik J. Soderblom, PhD

Co-Instructor: Will Thompson, PhD

Dr. Thompson received his Ph.D. in Analytical Chemistry from the University of North Carolina at Chapel Hill under Professor James Jorgenson in 2006. Following his Ph.D. he pursued a career in the application of UHPLC with high resolution mass spectrometry in the Disease and Biomarker Proteomics group of Dr. Arthur Moseley at GlaxoSmithKline, leaving GSK in 2008 to join Dr. Moseley in establishing the Duke Proteomics Core Facility. Dr. Thompson is currently the Assistant Director of the Duke Proteomics and Metabolomics Shared Resource, and a Research Assistant Professor in Department of Pharmacology and Cancer Biology. His research interests involve improving the throughput and sensitivity of unbiased and targeted proteomics and metabolomics approaches, with an eye towards generating assays which are easily translatable to the diagnostic laboratory.

Co-Instructor: Erik J. Soderblom, PhD

Dr. Erik Soderblom currently holds a joint appointment as a Research Scientist at Duke University’s Proteomics and Metabolomics Core Facility and as an Assistant Research Professor in Duke University’s Department of Cell Biology. He received a PhD in Molecular and Structural Biochemistry from North Carolina State University in 2008, prior to joining the Core Facility. His expertise lies in the utilization of various liquid chromatography-mass spectrometry (LC-MS) platforms to support a wide variety of research efforts from small scale basic science studies to larger scale clinical proteomics studies. Dr. Soderblom has over 10 years’ experience in differential expression proteomics using single and multi-dimensional nanoscale capillary chromatography coupled to high-resolution mass spectrometers, targeted proteomic analysis using triple quadrupole mass spectrometers (MRM) or high-resolution mass spectrometers (PRM), and comprehensive analysis of post-translational modifications such as phosphorylation and acetylation. As part of his normal responsibilities, Dr. Soderblom is involved in routine maintenance and troubleshooting of both LC and MS components across various instrument platforms, including design and execution of system suitability analysis to assess platform performance over time.

Co-Instructor: Christopher Shuford, PhD

Chris Shuford, Ph.D., is Technical Director for research and development at LabCorp’s Center for Esoteric Testing in Burlington, North Carolina. Chris received his B.S. in Chemistry & Physics at Longwood University and obtained his Ph.D. in Bioanalytical Chemistry from North Carolina State University under the tutelage of Professor David Muddiman, where his research focused on applications of nano-flow chromatography (<500 nL/min) for multiplexed peptide quantification using protein cleavage coupled with isotope dilution mass spectrometry (PC-IDMS). In 2012, Chris joined LabCorp’s research and development team where his efforts have focused on development of high-flow chromatographic methods (>1 mL/min) for multiplexed and single protein assays for clinical application.


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00

Overview: The general goal of the course is to enable practitioners of LC-MS/MS in the clinical laboratory to quickly recognize and diagnose specific problems with instrumentation, in order to decrease downtime and cost of repairs. The course includes ‘best practices’ for instrumentation installation, upkeep and maintenance, practical troubleshooting workflows for LC and MS, and will use problem sessions to reinforce skillsets. Although the course uses examples from specific instrumentation for demonstration, the content is geared to be vendor-neutral and applicable to all LC-MS systems. Additionally, we will provide an opportunity to have instrumentation troubleshooting questions from your laboratory addressed by the facilitators.

Brief outline of course content:

  • General “Best Practices” for Successful LC/MS Operation
    1. Best Practices; Getting Started on the Right Foot
    2. Breaking the System Down
    3. System Suitability! What is it, and how do I properly implement?
  • Focus on Liquid Chromatography
    1. Diagnostics using the “heartbeat” of your Chromatographic system
    2. Key System components and where things go wrong
    3. LC troubleshooting workflow
    4. Maintenance Intervals; service contract or do-it-yourself?
    5. Problem sets
  • Focus on Mass Spectrometry
    1. Discussion of Source, Transfer Optics, Vacuum and how each is critical to your system
    2. MS Troubleshooting workflow
    3. Ion optics cleaning and upkeep; what is ‘charging’?
    4. Problem sets
  • Integrated System
    1. Ionization
    2. System Communications
    3. Multi-vendor configurations
    4. Strategies to simplify
    5. Integrated real-lab problem scenarios and team exercises

While some basics of instrument component operation will be covered, it will be most beneficial to scientists with experience actively using LC-MS/MS as an analysis tool. While an in-depth discussion of how to operate each individual instrument is surely outside the scope of any short course, specific system setups will be used as examples and attendees will be encouraged to ask questions about specific systems in their own laboratories.

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$80$96$135
Academic / Non-Profit$240$288$405
Commercial / Industry$400$480$670
*Short Course Registration is separate from Conference Registration.



LC-MSMS 301 :: Development and Validation of Quantitative LC-MS/MS Assays for Use in Clinical Diagnostics

Level:3 (Advanced)
Prereqs:The target audience should have extensive familiarity with LC-MS/MS systems.
Location:TBA
Group:TBA
Instructor(s):Brian Rappold, Matthew Crawford and Chris Shuford

Brian Rappold is the Scientific Director at Essential Testing in Collinsville, Illinois. He is a renowned expert in the field of method development and validation of mass spectrometry assays for clinical diagnostic use, teaching courses on this subject at numerous scientific conferences. His extensive knowledge and clinical perspective has granted him several opportunities to present his research internationally on the topics of hydrophilic interaction liquid chromatography for bio-analysis, multiplexed mass spectrometric detection of amino acidopathies and the origins and solutions of ion suppression in electrospray ionization. He currently serves as the chair of Clinical Chemistry for the American Society for Mass Spectrometry (ASMS) and has served on the Metabolomics and Small Molecule Analysis/Toxicology Scientific Committee for The Association for Mass Spectrometry: Applications to the Clinical Laboratory (MSACL). His research interests include the realization of open-access mass spectrometric systems and antibody-capture/mass spectrometry applications to diagnostic medicine.

Matthew Crawford is a Researcher at LabCorp for the Mass Spectrometry Research and Development department. He received his Bachelors of Science in Biochemistry from California State University, Northridge in 2005. He entered the clinical lab space as a technician at LabCorp’s Esoterix Endocrinology and then moved to be a part of the corporate R&D team. His efforts are focused high-throughput small molecule method development, laboratory automation, and method validation (CAP, CLIA, and FDA Bioanalytical).

Chris Shuford, PhD is Technical Director for research and development at LabCorp’s Center for Esoteric Testing in Burlington, North Carolina. Chris received his B.S. in Chemistry & Physics at Longwood University and obtained his Ph.D. in Bioanalytical Chemistry from North Carolina State University under the tutelage of Professor David Muddiman, where his research focused on applications of nano-flow chromatography (<500 nL/min) for multiplexed peptide quantification using protein cleavage coupled with isotope dilution mass spectrometry (PC-IDMS). In 2012, Chris joined LabCorp’s research and development team where his efforts have focused on development of high-flow chromatographic methods (>1 mL/min) for multiplexed and single protein assays for clinical application.


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00

Overview: This course will briefly introduce the key aspects of the LC-MS/MS experimental workflow and then focus on processes and experimental designs for assay development and analytical validation of assays to be employed within clinical diagnostics.

The first day will describe method development in detail, including how-to guides for initial optimization of mass spectrometry systems, chromatographic development and sample preparation schemes. Techniques and technologies for streamlining analytical performance will also be described. Transitional experiments from development to validation will be discussed in detail to stress test methodologies prior to analytical validation.

Day two will cover all details pertinent in validation of LC-MS/MS analytical workflows. Experimental designs for all aspects of validation, putative acceptance criteria and analytical solutions will be shown. Key validation criteria of selectivity, carry-over, matrix effect, accuracy, precision, linearity, stability and inter-assay correlation will be described using multiple case studies.

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$80$96$135
Academic / Non-Profit$240$288$405
Commercial / Industry$400$480$670
*Short Course Registration is separate from Conference Registration.



MALDI 103 :: MALDI-MS Fundamentals and its Emerging Role in Pathology and Laboratory Medicine

Level:1-2 (Beginner - Intermediate)
Prereqs:Attendees should have some background in mass spectrometry and the basics of clinical and/or analytical chemistry, but this is not essential.
Location:TBA
Group:TBA
Instructor(s):Mark W. Duncan, PhD & Mari L. DeMarco, PhD

Co-Instructor: Mark W. Duncan, PhD

Biodesix Inc, Boulder, CO, USA

Mark Duncan’s research interests include clinical mass spectrometry, biomarker development and MALDI-MS. He is senior director of proteomic technology development at Biodesix Inc., and a visiting professor of medicine at the University of Colorado. He has extensive experience in the application of MALDI-MS, both as a qualitative and a quantitative tool.

Co-Instructor: Mari L. DeMarco, PhD DABCC FACB FCACB

Clinical Associate Professor, Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada; Michael Smith Foundation for Health Research Scholar

Mari DeMarco’s clinical service focuses on development of novel mass spectrometry assays in support of the clinical laboratory at St Paul's Hospital, Providence Health Care. Her research lab develops strategies for quantitative MS workflows for protein biomarkers and leverages MALDI-TOF MS instrumentation for assay design and implementation.


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00

Overview: This 2-day course introduces the key aspects of MALDI-MS, and current and emerging applications in medicine. This course is designed for those interested in developing, implementing and/or refining clinical applications of MALDI-MS.

In the first half of the course we will review MALDI-MS instrument fundamentals, sample requirements, workflows, and MALDI-MS data. We will highlight both the strengths and weaknesses of MALDI-MS. Thereafter, we will illustrate potential MALDI-MS applications to patient care from the perspective of pathology and laboratory medicine. In each section we will discuss relevant experimental design/operation, assay development, analytical validation and utility. Basic “how-to guides” will be presented.

The second half of the course is dedicated to a series of lectures from acknowledged experts in the application of MALDI-MS to relevant areas (e.g., clinical microbiology, clinical chemistry, genomics, and anatomic pathology). Each expert will give a targeted “real-world” lecture followed by a question-and-answer period. The course will conclude with an informal round-table discussion centered on topics of specific interest to the participants.

Specific topics covered in this course include the following:

  1. Introduction to MALDI-MS: how a MALDI-MS instrument works; advantages and disadvantages of MALDI-MS; variations on the MALDI theme – MALDI, SELDI, DIOS, DESI, LAESI, etc; different instrument configurations and analyzer types; advances in instrumentation; the basics of how to generate a spectrum and critical practical considerations (e.g., selection of matrix, laser power, shot number and other experimental variables).
  2. Applications in Clinical Microbiology: basic principles; strengths & weaknesses; the laboratory process; development and application of databases for automatic interpretation of MALDI spectra (concepts); data analysis; systems available for FDA approved pathogen identification and their features.
  3. Applications in Clinical Chemistry: clinical applications/potential of MALDI-TOF – qualitative applications; quantification by MALDI-MS; TOF-TOF quantification; related quantitative strategies; loss mass analyte analysis; approach to developing new assays by MALDI-TOF; sample processing (dilution, concentration, purification, etc.; transfer to MALDI plate; matrix choice and deposition; instrument parameters).
  4. Mass Spectrometry Imaging: Fundamentals; strengths & weaknesses; sample considerations; tissue processing and matrix application; clinical applications of MALDI imaging and profiling by MS.
  5. Future potential and prospects: The second half of the course will focus on advanced “real-world” applications of MALDI-MS presented by experts in their respective fields.

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$80$96$135
Academic / Non-Profit$240$288$405
Commercial / Industry$400$480$670
*Short Course Registration is separate from Conference Registration.



Metabolomics 201 :: Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis

Level:2 (Intermediate)
Prereqs:LC/MS hands on experience.
Location:TBA
Group:TBA
Instructor(s):Timothy Garrett, PhD & Erin Baker, PhD

Co-Instructor: Timothy Garrett, PhD

Dr. Garrett received his undergraduate degree from the University of Georgia in Chemistry graduating Summa Cum Laude with Highest Honors. As an undergraduate, he worked in the lab of Dr. I. Jonathan Amster on the characterization of bacterial proteins using MALDI-TOF. After 2 years in industry, he enrolled in the PhD program at the University of Florida working under the direction of Dr. Richard A. Yost. As a graduate student, he worked on the first imaging mass spectrometry based ion trap instrument through a partnership with Thermo and studied the disposition of phospholipids in brain tissue. He is currently an Associate Professor in the Department of Pathology at the University of Florida, where he is Associate Director for the Southeast Center for Integrated Metabolomics (SECIM).

Dr. Garrett has over 20 years of experience in the field of mass spectrometry spanning both instrument and application development. His background has involved the application of the ‘Omics from the development of MALDI based approaches to analyze proteins in bacteria to the development of imaging mass spectrometry instrumentation and application for analyzing small molecules in tissue specimens; his main focus is currently the translation of LC-HRMS based metabolomics to clinical diagnostics.

Prof Garrett is the author of over 60 publications and is an Editorial Board member for Clinical Mass Spectrometry and PLOSOne. His current interest are in the application of direct tissue analysis approaches such as MALDI, DESI, and LMJSSP as well as the use of high resolution mass spectrometry in metabolomics and routine diagnostics. He enjoys the interplay between technological advancement and clinical analysis providing unique opportunities and experiences to develop the future diagnostics.

Co-Instructor: Erin Baker, PhD

Dr. Erin Baker is a bioanalytical chemist with more than 16 years' experience utilizing ion mobility spectrometry in conjunction with mass spectrometry (IMS-MS) to study environmental and biological systems. In the last 10 years she has worked primarily on IMS-MS applications in the field of proteomics and more recently she has worked to optimize IMS-MS metabolomic, glycomic and lipidomic separations. Her research involves the development and evaluation of high-throughput IMS-MS, SPE-IMS-MS and LC-IMS-MS analyses to quickly study numerous samples in a short time period without losing valuable biological information, as well as assessing the number and quality of features detected with IMS-MS for comparison with existing MS platforms. Dr. Baker is also presently working with the PNNL Informatics team to design and implement software tools that automatically analyze the complex multidimensional SPE-IMS-MS and LC-IMS-MS data.


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00

Overview: Metabolomics describes the analysis of the small molecules present in our body and ingested from the surrounding environment (i.e. drugs, pesticides, etc.). The analysis of these metabolites has recently been utilized to discover new markers of disease and perturbed metabolic pathways. Metabolomic analyses can be performed with either targeted or untargeted measurements. In targeted studies, only a small subset of metabolites is analyzed and this prevalent in clinical analyses for measurements such as those for newborn screening. Untargeted measurements, however, study all possible small molecules in a single injection and heavily rely on the use of high resolution mass spectrometry to precisely measure the m/z values across many samples. Untargeted measurements are almost always coupled to either gas or liquid chromatography or ion mobility as the retention time or mobility provides an important secondary distinguishing characteristic of each specific metabolite. It is expected that both targeted and untargeted metabolomic measurements will have an important place in future clinical studies.

Given the growth of metabolomics over the past several years, the use of high resolution mass spectrometry has rapidly progressed. High resolution approaches to measure small molecules offer several advantages for clinical analyses such as confirmation via accurate mass of the precursor and product ions and an evaluation of the isotopic abundance. This short course will cover the application of high resolution mass spectrometry to both quantitative and semi-quantitative analyses with a focus on metabolomics.

Topics in this short course include: mass accuracy for identification, tandem mass spectrometry, quantitative aspects of high resolution mass spectrometry, identifying and measuring the metabolome, statistical analysis, ion mobility spectrometry, and liquid and gas chromatography coupled to high resolution MS.

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$80$96$135
Academic / Non-Profit$240$288$405
Commercial / Industry$400$480$670
*Short Course Registration is separate from Conference Registration.



Proteomics 201 :: Clinical Proteomics

Level:2-3 (Intermediate - Advanced)
Prereqs:Practical knowledge of quantitative mass spectrometry.
Location:TBA
Group:TBA
Instructor(s):Andy Hoofnagle, MD, PhD, Cory Bystrom, PhD & Chris Shuford, PhD

Co-Instructor: Andy Hoofnagle, MD, PhD

Dr. Hoofnagle's laboratory focuses on the precise quantification of recognized protein biomarkers in human plasma using LC-MRM/MS. In addition, they have worked to develop novel assays for the quantification of small molecules in clinical and research settings. His laboratory also studies the role that the systemic inflammation plays in the pathophysiology of obesity, diabetes, and cardiovascular disease.

Co-Instructor: Cory Bystrom, PhD

Dr. Cory Bystrom serves as Director of Research and Development at Cleveland HeartLab where he is responsible for novel biomarker identification, validation and commercialization with an emphasis on quantitative biological mass spectrometry. Dr. Bystrom has over a decade of experience as a laboratory leader and chemist. Prior to joining CHL, he was at Quest Diagnostics Nichols Institute as associate director of research and development with responsibilities for development of tests and identification of analytical strategies for commercialization of new biomarkers. He also has held research and development roles at Oregon Health Science University, Fonterra, and Pharmacia and Upjohn.

Co-Instructor: Christopher Shuford, PhD

Chris Shuford, Ph.D., is Technical Director for research and development at LabCorp’s Center for Esoteric Testing in Burlington, North Carolina. Chris received his B.S. in Chemistry & Physics at Longwood University and obtained his Ph.D. in Bioanalytical Chemistry from North Carolina State University under the tutelage of Professor David Muddiman, where his research focused on applications of nano-flow chromatography (<500 nL/min) for multiplexed peptide quantification using protein cleavage coupled with isotope dilution mass spectrometry (PC-IDMS). In 2012, Chris joined LabCorp’s research and development team where his efforts have focused on development of high-flow chromatographic methods (>1 mL/min) for multiplexed and single protein assays for clinical application.


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00

Overview: This course will explore the background of clinical proteomics and approaches to method development and validation. We will provide the motivation for using mass spectrometry to quantify proteins in clinical research and in clinical care. The promise of mass spectrometry to improve the accuracy and precision of results is only realized with robust methods. In order to prepare participants to begin to develop their own robust methods for quantification we will focus on the following topics:

  1. Why mass spec for peptides and proteins
  2. Optimization of digestion and other aspects of the method
  3. Internal standards
  4. Calibration
  5. Immunoaffinity enrichment
  6. Validation
  7. Quality control
  8. Case studies

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$80$96$135
Academic / Non-Profit$240$288$405
Commercial / Industry$400$480$670
*Short Course Registration is separate from Conference Registration.



Sample Prep 201 :: Sample Preparation and Alternative Matrices for LC-MS Assays

Level:Beginner / Intermediate
Prereqs:Familiarity with LC-MS/MS.
Location:TBA
Group:TBA
Instructor(s):William Clarke, PhD & Mark Marzinke, PhD


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Not in SessionSTARTS <
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00

Overview: This course will encompass various sample preparation approaches used for LC-MS assays. The course will highlight not only the importance of sample processing in the clinical laboratory environment, but also illustrate the “fit for purpose” application of processing techniques in clinical mass spectrometry. This course will also discuss the theory behind different specimen preparation methods, strengths and weaknesses of each approach, as well as opportunities for automation. The first 8 hours will serve as a primer of the role of upfront sample management, utilizing examples in blood and urine specimen sources. There will also be an introduction to the application of LC-MS approaches in alternative matrices. The second 8 hours will elaborate on the foundations established in the first half, and expand into newer technologies and automated alternatives for sample processing.

Specific topics to be covered include:

  1. Pain points in clinical LC-MS
  2. Overview of specimen processing in laboratory medicine
  3. Off-line sample processing
  4. On-line sample processing
  5. Analysis of blood and urine
  6. LC-MS of tissue specimens
  7. Alternate body fluid specimens (e.g. CSF, breast milk, etc.)
  8. Intracellular and metabolite analysis
  9. Dried specimens as matrices
  10. Automation of sample processing

Topics will be covered through lecture, Q&A, Case Studies, and small group exercises.

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$60$70$100
Academic / Non-Profit$180$215$300
Commercial / Industry$300$360$500
*Short Course Registration is separate from Conference Registration.



Forensic Toxicology 101 :: Basic Forensic Toxicology

Level:Beginner
Prereqs:None.
Location:TBA
Group:B
Instructor(s):Jarrad Wagner, Ph.D., F-ABFT, Allison Veitenheimer, Ph.D., Russell Lewis, Ph.D., F-ABFT & Robert Johnson, PhD, F-ABFT

Dr. Jarrad Wagner is a Professor of Forensic Sciences at Oklahoma State University Center for Health Sciences (OSU CHS), where he is the Director of the Forensic Toxicology and Trace Laboratory (FTTL). His works primarily with triple quadrupole LC/MS/MS instruments and supporting forensic and clinical laboratories in method development, validation and training. Dr. Wagner is a Fellow of the American Board of Forensic Toxicology and also a Fellow in the Criminalistics section of the American Academy of Forensic Sciences. He serves as a member of the AAFS/SOFT Drugs and Driving Committee on the Oral Fluid subcommittee, is a member of the National Safety Council Alcohol, Drugs and Impairment Division and Vice Chair of the Oklahoma State Board of Tests for Alcohol and Drug Influence. In addition to his role as faculty, he has also formerly been a chemist, a forensic scientist and reserve police officer.

Dr. Allison Veitenheimer is the Forensic Toxicology Team Lead at the Federal Aviation Administration’s Bioaeronautical Sciences Research Laboratory at the Civil Aerospace Medical Institute in Oklahoma City, Oklahoma. There, she supervises and performs toxicological analyses of civil aircraft accident fatalities and high-visibility NTSB surface accidents. Dr. Veitenheimer also serves as a member of the graduate faculty at Oklahoma State University Center for Health Sciences, School of Forensic Sciences. She has experience in method development and validation with both LC/MS/MS and GC/MS in toxicology, drug, and explosives analysis. Dr. Veitenheimer’s current research activities include developing new methods for the analysis of drugs in postmortem specimens. She has authored/co-authored several scientific journal articles as well as presented at numerous conferences and workshops. Dr. Veitenheimer holds a Ph.D. in Veterinary Biomedical Sciences and a Master of Science in Forensic Sciences with an emphasis in toxicology from Oklahoma State University and a Bachelor of Science from Baylor University. She also holds a Graduate Certificate in Interdisciplinary Toxicology from Oklahoma State University.

Dr. Lewis is the Forensic Sciences Supervisor at the Federal Aviation Administration’s Forensic Sciences Laboratory at the Civil Aerospace Medical Institute in Oklahoma City, Oklahoma. There he oversees the toxicological analyses of civil aircraft accident fatalities and select NTSB surface accidents, acts as the certifying toxicologist, and provides expert testimony for casework and final case reports. Dr. Lewis also serves as an Adjunct Clinical Assistant Professor for Oklahoma State University Center for Health Sciences, School of Forensic Sciences. Dr. Lewis’ current research activities include investigating postmortem ethanol formation and developing new methods for the analysis of drugs in postmortem specimens. Dr. Lewis has authored/co-authored over 85 scientific journal articles /book chapters/government reports. Prior to his life as a forensic scientist Dr. Lewis received a Bachelor of Science in Chemistry and Mathematics dual major in 1991 (Black Hills State University, South Dakota), a Master’s in Chemistry in 1994 (University of Oklahoma), a Specialization in Neuroscience in 1995 (University of Oklahoma Health Sciences Center), and a Ph.D. in Analytical Chemistry in 1997 (the University of Oklahoma).

Dr. Robert Johnson is the Chief Toxicologist at the Tarrant County Medical Examiner’s Office in Fort Worth, TX.


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
> ENDS
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Not in Session Not in Session

Overview: This 1-day, 8-hour course aims to make scientists familiar with the basic concepts of forensic toxicology. It is presented at the beginner level and is ideal for attendees that wish to learn more about the forensic toxicology landscape. Specific topics that are addressed include:

  1. Intro to Forensic Toxicology
    • History of Forensic Toxicology
    • Analytical Techniques in Forensic Toxicology (Colorimetric, Immunoassay, Hyphenated Chromatography-Detection)
    • Modern Divisions
  2. Human Performance Toxicology
    • Description, Specimen Types, Analytical Techniques, Interpretation Challenges, Case Studies
    • Ethanol
    • DUID
  3. Postmortem Toxicology (Description, Specimen Types, Analytical Techniques, Interpretation Challenges, Case Studies)
  4. Workplace Drug Testing (Description, Specimen Types, Analytical Techniques, Interpretation Challenges, Case Studies)
  5. Quality Assurance in Forensic Toxicology (Validation, Accreditation and Certification)

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$40$48$70
Academic / Non-Profit$120$144$200
Commercial / Industry$250$300$350
*Short Course Registration is separate from Conference Registration.



Forensic Toxicology 201 :: Advanced Forensic Toxicology

Level:Intermediate/Advanced
Prereqs:Forensic Toxicology 101 recommended, not required.
Location:TBA
Group:B
Instructor(s):Jarrad Wagner, Ph.D., F-ABFT, Allison Veitenheimer, Ph.D., Russell Lewis, Ph.D., F-ABFT & Robert Johnson, PhD, F-ABFT

Dr. Jarrad Wagner is a Professor of Forensic Sciences at Oklahoma State University Center for Health Sciences (OSU CHS), where he is the Director of the Forensic Toxicology and Trace Laboratory (FTTL). His works primarily with triple quadrupole LC/MS/MS instruments and supporting forensic and clinical laboratories in method development, validation and training. Dr. Wagner is a Fellow of the American Board of Forensic Toxicology and also a Fellow in the Criminalistics section of the American Academy of Forensic Sciences. He serves as a member of the AAFS/SOFT Drugs and Driving Committee on the Oral Fluid subcommittee, is a member of the National Safety Council Alcohol, Drugs and Impairment Division and Vice Chair of the Oklahoma State Board of Tests for Alcohol and Drug Influence. In addition to his role as faculty, he has also formerly been a chemist, a forensic scientist and reserve police officer.

Dr. Allison Veitenheimer is the Forensic Toxicology Team Lead at the Federal Aviation Administration’s Bioaeronautical Sciences Research Laboratory at the Civil Aerospace Medical Institute in Oklahoma City, Oklahoma. There, she supervises and performs toxicological analyses of civil aircraft accident fatalities and high-visibility NTSB surface accidents. Dr. Veitenheimer also serves as a member of the graduate faculty at Oklahoma State University Center for Health Sciences, School of Forensic Sciences. She has experience in method development and validation with both LC/MS/MS and GC/MS in toxicology, drug, and explosives analysis. Dr. Veitenheimer’s current research activities include developing new methods for the analysis of drugs in postmortem specimens. She has authored/co-authored several scientific journal articles as well as presented at numerous conferences and workshops. Dr. Veitenheimer holds a Ph.D. in Veterinary Biomedical Sciences and a Master of Science in Forensic Sciences with an emphasis in toxicology from Oklahoma State University and a Bachelor of Science from Baylor University. She also holds a Graduate Certificate in Interdisciplinary Toxicology from Oklahoma State University.

Dr. Lewis is the Forensic Sciences Supervisor at the Federal Aviation Administration’s Forensic Sciences Laboratory at the Civil Aerospace Medical Institute in Oklahoma City, Oklahoma. There he oversees the toxicological analyses of civil aircraft accident fatalities and select NTSB surface accidents, acts as the certifying toxicologist, and provides expert testimony for casework and final case reports. Dr. Lewis also serves as an Adjunct Clinical Assistant Professor for Oklahoma State University Center for Health Sciences, School of Forensic Sciences. Dr. Lewis’ current research activities include investigating postmortem ethanol formation and developing new methods for the analysis of drugs in postmortem specimens. Dr. Lewis has authored/co-authored over 85 scientific journal articles /book chapters/government reports. Prior to his life as a forensic scientist Dr. Lewis received a Bachelor of Science in Chemistry and Mathematics dual major in 1991 (Black Hills State University, South Dakota), a Master’s in Chemistry in 1994 (University of Oklahoma), a Specialization in Neuroscience in 1995 (University of Oklahoma Health Sciences Center), and a Ph.D. in Analytical Chemistry in 1997 (the University of Oklahoma).

Dr. Robert Johnson is the Chief Toxicologist at the Tarrant County Medical Examiner’s Office in Fort Worth, TX.


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Not in Session Not in SessionLunch
Monday
12:00-13:00
STARTS <
Monday
13:00-17:00
> ENDS
Tuesday
8:00-12:00

Overview: This 1-day, 8-hour course aims to make scientists familiar with the issues surrounding various classes of drugs and their role in forensic toxicology scenarios. Current drug use trends in the United States will be discussed, along with analytical recommendations. It is presented at the intermediate to advanced level and is ideal for attendees that wish to gain more advanced knowledge about forensic toxicology analytes.

BLOCK 1

  1. Cannabinoids
    • Prevalence
    • Pharmacology/Toxicology
    • Human Performance Toxicology
      • Interpretation challenges
      • Case Studies
    • Post-Mortem Toxicology
      • Interpretation challenges
      • Case Studies
  2. Stimulants/Sympathomimetics
    • Prevalence
    • Pharmacology/Toxicology
    • Human Performance Toxicology
    • Interpretation challenges
    • Case Studies
    • Post-Mortem Toxicology
    • Interpretation challenges
    • Case Studies
  3. Benzodiazepines
    • Prevalence
    • Pharmacology/Toxicology
    • Human Performance Toxicology
      • Interpretation challenges
      • Case Studies
    • Post-Mortem Toxicology
      • Interpretation challenges
      • Case Studies
BLOCK 2

  1. Opiates/Opioids
    • Prevalence
    • Pharmacology/Toxicology
    • Human Performance Toxicology
      • Interpretation challenges
      • Case Studies
    • Post-Mortem Toxicology
      • Interpretation challenges
      • Case Studies
  2. Novel Psychoactive Substances (NPS)
    • Prevalence
    • Pharmacology/Toxicology
    • Human Performance Toxicology
      • Interpretation challenges
      • Case Studies
    • Post-Mortem Toxicology
      • Interpretation challenges
      • Case Studies
  3. Drug-Facilitated Crimes

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$40$48$75
Academic / Non-Profit$120$144$200
Commercial / Industry$250$300$350
*Short Course Registration is separate from Conference Registration.