Accelerating the Implementation of Mass Spectrometry in the Clinical Lab

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MSACL 2019 US

Palm Springs: Mar 31 - Apr 4

UNITED STATES 2019

Help Us Reach Our Educational Support Goal of $40,000
Educational Travel Grants supported in part by:
Brian Kelly, Amadeo Pesce, Danyel Tacker
&

Short Courses

MSACL hosts a diverse offering of Short Courses.

Short Courses will occur over the first three days of MSACL (Sunday, Monday, Tuesday of September 9 - 11).

Courses are NOT replicated on different days. They are single courses that span multiple days.

Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
LC-MS
LC-MSMS 201
Understanding and Optimization of LC-MS/MS to Develop Successful Methods for Identification and Quantitation in Complex Matrices
Robert D. Voyksner, PhD
TBD
STARTS <
Sunday
14:00-18:00
Monday
9:00-13:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
> ENDS
Tuesday
9:00-12:30
MALDI
MALDI 103
MALDI-MS Fundamentals and its Emerging Role in Pathology and Laboratory Medicine
Mark W. Duncan, PhD & Mari L. DeMarco, PhD
TBD
STARTS <
Sunday
14:00-18:00
Monday
9:00-13:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
> ENDS
Tuesday
9:00-12:30
Metabolomics
Metabolomics 201
Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis
Timothy Garrett, PhD & Erin Baker, PhD
TBD
STARTS <
Sunday
14:00-18:00
Monday
9:00-13:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
> ENDS
Tuesday
9:00-12:30



LC-MSMS 201 :: Understanding and Optimization of LC-MS/MS to Develop Successful Methods for Identification and Quantitation in Complex Matrices

Level:2 (Intermediate)
Prerequisites:General knowledge of laboratory techniques associated with HPLC and mass spectrometry and some hands-on experience with running an LC/MS system.
Location:TBD
Instructor(s):Robert D. Voyksner, PhD

Instructor: Robert D. Voyksner, PhD

Dr. Robert D. Voyksner received his B.S. in Chemistry at Canisius College in 1978 and his Ph.D. at the University of North Carolina at Chapel Hill in 1982. He was employed at Research Triangle Institute (RTI) from 1983-2001 as the director of the mass spectrometry facility and has been responsible for developing extraction, separation and mass spectrometric methods for biologically and environmentally significant compounds. His work earned him the Presidents Award, the highest award within RTI. In 2001 he co-founded LCMS Limited in Raleigh, NC and has been the President of the company to date. Under his direction LCMS Limited is working on technological advancements in LC/MS, offering services to pharmaceutical, clinical and agrochemical industry for solving unique problems by LC/MS/MS and offering training in LC/MS/MS and MS/MS interpretation and on LC/MS/MS instrumentation. Dr Voyksner is also an Adjunct professor at the North Carolina a School of Vetinary Medicine and at The University of North Carolina School of Pharmacy.

Dr. Voyksner's research in mass spectrometry has resulted in over 230 publications and presentations, primarily in the area of HPLC/MS. He has served on the Board of Directors for The American Society For Mass Spectrometry (ASMS), is on the organization committee for The Montreux LC/MS Symposium and was the organizer for the 1995, 1999, 2003 and 2007 Montreux LC/MS Symposia. Dr. Voyksner has taught over 100 courses on LC/MS, CE/MS and CID interpretation during the past 10 years for ASMS, pharmaceutical companies; ISSX, PBA, HPCE and HPLC focused meetings.

Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
9:00-13:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
> ENDS
Tuesday
9:00-12:30

Overview: This course is designed for the chromatographer / mass spectrometrist who want to be successful in developing methods, method optimization and solving problems using LC/MS/MS. The course covers the atmospheric pressure ionization (API) techniques of electrospray, pneumatically assisted electrospray and atmospheric pressure chemical ionization (APCI) and atmospheric pressure photo ionization (APPI) using single quadrupole, triple quadrupole, time-of-flight and ion trap mass analyzers.

Discussions of sample preparation and chromatography will target method development and optimization for the analysis of "real-world" samples by LC/MS/MS.

The course highlights the following topics with respect to optimization a method to achieve the best sensitivity, specificity and sample throughput:

  1. Optimization ionization in API techniques,
  2. understanding and minimizing matrix suppression,
  3. relative merits of various LC column lengths, particle sizes and column diameters for LC/MS/MS analysis,
  4. introduction into the interpretation of MS/MS spectra,
  5. important issues in LC/MS/MS quantitation, and
  6. optimization of an quantitative analysis.

Applications of LC/MS/MS to analyze compounds of clinical interest in biological matrices will be discussed throughout the course to emphasize the topics covered.

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$80$96$135
Academic / Non-Profit$240$288$405
Commercial / Industry$400$480$670
*Short Course Registration is separate from Conference Registration.



MALDI 103 :: MALDI-MS Fundamentals and its Emerging Role in Pathology and Laboratory Medicine

Level:1-2 (Beginner - Intermediate)
Prerequisites:Attendees should have some background in mass spectrometry and the basics of clinical and/or analytical chemistry, but this is not essential.
Location:TBD
Instructor(s):Mark W. Duncan, PhD & Mari L. DeMarco, PhD

Co-Instructor: Mark W. Duncan, PhD

Biodesix Inc, Boulder, CO, USA

Mark Duncan’s research interests include clinical mass spectrometry, biomarker development and MALDI-MS. He is senior director of proteomic technology development at Biodesix Inc., and a visiting professor of medicine at the University of Colorado. He has extensive experience in the application of MALDI-MS, both as a qualitative and a quantitative tool.

Co-Instructor: Mari L. DeMarco, PhD DABCC FACB FCACB

Clinical Associate Professor, Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada; Michael Smith Foundation for Health Research Scholar

Mari DeMarco’s clinical service focuses on development of novel mass spectrometry assays in support of the clinical laboratory at St Paul's Hospital, Providence Health Care. Her research lab develops strategies for quantitative MS workflows for protein biomarkers and leverages MALDI-TOF MS instrumentation for assay design and implementation.

Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
9:00-13:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
> ENDS
Tuesday
9:00-12:30

Overview: This 2-day course introduces the key aspects of MALDI-MS, and current and emerging applications in medicine. This course is designed for those interested in developing, implementing and/or refining clinical applications of MALDI-MS.

In the first half of the course we will review MALDI-MS instrument fundamentals, sample requirements, workflows, and MALDI-MS data. We will highlight both the strengths and weaknesses of MALDI-MS. Thereafter, we will illustrate potential MALDI-MS applications to patient care from the perspective of pathology and laboratory medicine. In each section we will discuss relevant experimental design/operation, assay development, analytical validation and utility. Basic “how-to guides” will be presented.

The second half of the course is dedicated to a series of lectures from acknowledged experts in the application of MALDI-MS to relevant areas (e.g., clinical microbiology, clinical chemistry, genomics, and anatomic pathology). Each expert will give a targeted “real-world” lecture followed by a question-and-answer period. The course will conclude with an informal round-table discussion centered on topics of specific interest to the participants.

Specific topics covered in this course include the following:

  • Introduction to MALDI-MS: how a MALDI-MS instrument works; advantages and disadvantages of MALDI-MS; variations on the MALDI theme – MALDI, SELDI, DIOS, DESI, LAESI, etc; different instrument configurations and analyzer types; advances in instrumentation; the basics of how to generate a spectrum and critical practical considerations (e.g., selection of matrix, laser power, shot number and other experimental variables).
  • Applications in Clinical Microbiology: basic principles; strengths & weaknesses; the laboratory process; development and application of databases for automatic interpretation of MALDI spectra (concepts); data analysis; systems available for FDA approved pathogen identification and their features.
  • Applications in Clinical Chemistry: clinical applications/potential of MALDI-TOF – qualitative applications; quantification by MALDI-MS; TOF-TOF quantification; related quantitative strategies; loss mass analyte analysis; approach to developing new assays by MALDI-TOF; sample processing (dilution, concentration, purification, etc.; transfer to MALDI plate; matrix choice and deposition; instrument parameters).
  • Mass Spectrometry Imaging: Fundamentals; strengths & weaknesses; sample considerations; tissue processing and matrix application; clinical applications of MALDI imaging and profiling by MS.
  • Future potential and prospects: The second half of the course will focus on advanced “real-world” applications of MALDI-MS presented by experts in their respective fields.

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$80$96$135
Academic / Non-Profit$240$288$405
Commercial / Industry$400$480$670
*Short Course Registration is separate from Conference Registration.



Metabolomics 201 :: Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis

Level:2 (Intermediate)
Prerequisites:LC/MS hands on experience.
Location:TBD
Instructor(s):Timothy Garrett, PhD & Erin Baker, PhD

Co-Instructor: Timothy Garrett, PhD

TBA

Co-Instructor: Erin Baker, PhD

Dr. Erin Baker is a bioanalytical chemist with more than 16 years' experience utilizing ion mobility spectrometry in conjunction with mass spectrometry (IMS-MS) to study environmental and biological systems. In the last 10 years she has worked primarily on IMS-MS applications in the field of proteomics and more recently she has worked to optimize IMS-MS metabolomic, glycomic and lipidomic separations. Her research involves the development and evaluation of high-throughput IMS-MS, SPE-IMS-MS and LC-IMS-MS analyses to quickly study numerous samples in a short time period without losing valuable biological information, as well as assessing the number and quality of features detected with IMS-MS for comparison with existing MS platforms. Dr. Baker is also presently working with the PNNL Informatics team to design and implement software tools that automatically analyze the complex multidimensional SPE-IMS-MS and LC-IMS-MS data.

Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
9:00-13:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
> ENDS
Tuesday
9:00-12:30

Overview: Metabolomics describes the analysis of the small molecules present in our body and ingested from the surrounding environment (i.e. drugs, pesticides, etc.). The analysis of these metabolites has recently been utilized to discover new markers of disease and perturbed metabolic pathways. Metabolomic analyses can be performed with either targeted or untargeted measurements. In targeted studies, only a small subset of metabolites is analyzed and this prevalent in clinical analyses for measurements such as those for newborn screening. Untargeted measurements, however, study all possible small molecules in a single injection and heavily rely on the use of high resolution mass spectrometry to precisely measure the m/z values across many samples. Untargeted measurements are almost always coupled to either gas or liquid chromatography or ion mobility as the retention time or mobility provides an important secondary distinguishing characteristic of each specific metabolite. It is expected that both targeted and untargeted metabolomic measurements will have an important place in future clinical studies.

Given the growth of metabolomics over the past several years, the use of high resolution mass spectrometry has rapidly progressed. High resolution approaches to measure small molecules offer several advantages for clinical analyses such as confirmation via accurate mass of the precursor and product ions and an evaluation of the isotopic abundance. This short course will cover the application of high resolution mass spectrometry to both quantitative and semi-quantitative analyses with a focus on metabolomics.

Topics in this short course include: mass accuracy for identification, tandem mass spectrometry, quantitative aspects of high resolution mass spectrometry, identifying and measuring the metabolome, statistical analysis, ion mobility spectrometry, and liquid and gas chromatography coupled to high resolution MS.

EarlyBird
Deadline
Jan 31, 2019
Regular
Deadline
Feb 20, 2019
After
Feb 20, 2019
Student / Post-Doc (Trainee)$80$96$135
Academic / Non-Profit$240$288$405
Commercial / Industry$400$480$670
*Short Course Registration is separate from Conference Registration.