Dr. Grant earned a first-class honors degree in Industrial Chemistry from Cardiff University and a PhD in Chromatographic and Mass Spectrometric technologies from the University of Swansea, Wales, United Kingdom. He continued his scientific training in various industrial settings, which have included senior scientist at GSK, Principal scientist at Cohesive Technologies, Technical director at Eli Lilly, and Director of Mass Spectrometry at Esoterix Endocrinology. Dr Grant is currently the Vice President of Research and Development and co-discipline director for Mass spectrometry at Labcorp. Dr Grant has pioneered the use of direct injection technologies, chromatographic systems multiplexing, microsampling, utility of automation, and other new analytical platforms in direct patient care. His research goals are focused upon improvements in speed, sensitivity, and quality of liquid chromatography with tandem mass spectrometric (LC-MS/MS) analytical systems and assays. Dr Grant has been awarded 100 patents and received both the MSACL Distinguished contribution award and ASMS AL Yergey “Unsung Hero” Award in 2024 for his contributions to Clinical Diagnostics using Mass Spectrometry.

Central to determining assay quality and results reporting apriori is the fundamental belief that one is selectively measuring the analyte of choice. The use of transition ratio monitoring, while somewhat known to many users of tandem mass spectrometry, still provides a multitude of discreet challenges in the development, use and interrogation of chromatographic data. Historical rules based review, driven by qualifier transition yield relative to the quantifier transition fails to provide the appropriate quality considerations based on meaningful data. This pair of 90 minute presentations will include an arc of developmental understanding, from initial assessments (including favoring selectivity over sensitivity), validation of ion ratio's (sample types and concentrations) and use of transition ratio's (multi-level, non-symmetrical and statistically based).

Take home "Pearls" include:
1. How to select appropriate transitions for use in transition ratio monitoring - including tools to enhance response function for low intensity transitions
2. How to improve the precision of Transition ratio's through scheduling and appropriate dwell times
3. How to design transition ratio tolerances to enhance medical utility and including when to ignore the qualifier transition

Dr. Grant earned a first-class honors degree in Industrial Chemistry from Cardiff University and a PhD in Chromatographic and Mass Spectrometric technologies from the University of Swansea, Wales, United Kingdom. He continued his scientific training in various industrial settings, which have included senior scientist at GSK, Principal scientist at Cohesive Technologies, Technical director at Eli Lilly, and Director of Mass Spectrometry at Esoterix Endocrinology. Dr Grant is currently the Vice President of Research and Development and co-discipline director for Mass spectrometry at Labcorp. Dr Grant has pioneered the use of direct injection technologies, chromatographic systems multiplexing, microsampling, utility of automation, and other new analytical platforms in direct patient care. His research goals are focused upon improvements in speed, sensitivity, and quality of liquid chromatography with tandem mass spectrometric (LC-MS/MS) analytical systems and assays. Dr Grant has been awarded 100 patents and received both the MSACL Distinguished contribution award and ASMS AL Yergey “Unsung Hero” Award in 2024 for his contributions to Clinical Diagnostics using Mass Spectrometry.

Note: This is Part 2 of a two part session.
Central to determining assay quality and results reporting apriori is the fundamental belief that one is selectively measuring the analyte of choice. The use of transition ratio monitoring, while somewhat known to many users of tandem mass spectrometry, still provides a multitude of discreet challenges in the development, use and interrogation of chromatographic data. Historical rules based review, driven by qualifier transition yield relative to the quantifier transition fails to provide the appropriate quality considerations based on meaningful data. This pair of 90 minute presentations will include an arc of developmental understanding, from initial assessments (including favoring selectivity over sensitivity), validation of ion ratio's (sample types and concentrations) and use of transition ratio's (multi-level, non-symmetrical and statistically based).

Take home "Pearls" include:
1. How to select appropriate transitions for use in transition ratio monitoring - including tools to enhance response function for low intensity transitions
2. How to improve the precision of Transition ratio's through scheduling and appropriate dwell times
3. How to design transition ratio tolerances to enhance medical utility and including when to ignore the qualifier transition

Sindhu Nair is a seasoned Quality Consultant and CEO of Q Boost Inc., with extensive experience in quality management and software development. She has successfully implemented ISO and CLIA standards across various labs and is an active contributor to the development of ISO 15189 standards. Sindhu also performs audits for ISO 17025, ISO 9001, ISO 13485, and ISO 15189 standards.

In her role at Q Boost Inc., Sindhu provides quality consultation services to research labs and start-ups, performs ISO audits, and develops quality-compliant e-QMS systems. She is an active member of various professional organizations, including the Metabolomics Quality Assurance and Quality Control Consortium (mQACC), the Standards Council of Canada (SCC), and the Canadian Standards Association (CSA).

Sindhu is recognized for her leadership, problem-solving skills, and passion for quality system development, consistently enhancing laboratory standards and practices.

Dr. Yamilé López Hernández (Ph.D in Health Sciences) is the Technical Director of the Clinical Metabolomics Laboratory at the Universidad Autónoma de Zacatecas, Mexico and a Research Associate at Wishart´s lab (University of Alberta, Edmonton, Canada). As head of the Metabolomics and Proteomics Facility at the Autonomous University of Zacatecas, she has built one of the region's premier laboratories for biomarker discovery, which uses untargeted and targeted metabolomics techniques to identify critical biomarkers for diseases such as COVID-19, diabetes, and cancer. By building this facility from the ground up, Dr. López Hernández has positioned her lab as a leading institution in Mexico for biomarker research, supporting both national and international collaborations. Her contributions are groundbreaking for Latin America, where access to cutting-edge technologies like mass spectrometry has historically been limited. She is a member of the Board of Directors of the North American Metabolomics Society (MANA) and member of the Metabolomics Society and the Latin American Metabolite Profiling Society.

By the end of this 3-hour workshop, participants will be able to:

Understand ISO 15189 Requirements: Clearly articulate the key requirements and standards of ISO 15189 specific to metabolomic labs, including documentation, quality control, and management responsibilities.

Implement Best Practices: Apply best practices and strategies for establishing a quality management system (QMS) that meets ISO 15189 standards, including risk management, external and internal quality control, corrective actions, and continual improvement processes.

Prepare for Certification: Develop a step-by-step plan to prepare their metabolomic lab for ISO 15189 certification, including conducting internal audits, addressing non-conformities, and engaging with accrediting bodies.

Utilize Tools and Resources: Identify and utilize tools, resources, and software solutions that can aid in meeting ISO 15189 requirements, enhancing lab efficiency, and ensuring compliance.

Troubleshoot Common Challenges: Recognize and troubleshoot common challenges and obstacles faced during the ISO 15189 certification process, with special emphasis on developing a proper method validation protocol and implementing an effective training and competency program. Gain practical guidelines and solutions to overcome these issues.