MSACL 2023 Program

MSACL 2023 : Conference Program

Monterey, CA • April 2-7, 2023

2023 Sponsors

Abstract Clinical Use Status Key
= Emerging. More than 5 years before clinical availability. (24.37%, 2023)
= Expected to be clinically available in 1 to 4 years. (39.50%, 2023)
= Clinically available now. (36.13%, 2023)


Sunday

Sunday
1600
2000
Reg Desk Open for Early Badge Pick-Up
@ Exhibit Hall - Serra
2044
Short Course : LC-MSMS 101 : Getting Started with Quantitative LC-MSMS in the Diagnostic Laboratory
@ De Anza 3

Judy Stone, MT (ASCP), PhD, DABCC
Clinical Chemist (retired)

Jacqueline Hubbard, PhD, DABCC
Three Rivers Diagnostics

Grace van der Gugten, B.Sc. Chemistry
Alberta Precision Laboratories

Lorin Bachmann, PhD, DABCC
VCU Health System

Deborah French, PhD, DABCC (CC, TC)
UCSF

Adina Badea, PhD, DABCC
Lifespan/Rhode Island Hospital & the Warren Alpert Medical School of Brown University

Raymond Suhandynata, PhD DABCC
University of California, San Diego


IN-PERSON (Judy Stone, Jacqueline Hubbard) & ON-LINE (Grace van der Gugten, Deborah French, Lorin Bachmann)

REGISTER FOR VIRTUAL LC-MSMS 101 -- this virtual course will take place with the MSACL 2023 Monterey students who are onsite, except that you will have three online guides (Grace van der Gugten, Lorin Bachmann and Deborah French).

Course Schedule

Segment 1 : Sunday 17:00 - 18:30 (1.5 h)
Segment 2 : Monday 08:00 - 12:00 (4 h)
Segment 3 : Monday 13:30 - 17:30 (4 h)
Segment 4 : Tuesday 08:00 - 12:00 (4 h)
Segment 5 : Tuesday 13:30 - 16:00 (2.5 h)

Total Contact Hours: 16.00

---------------

Pre-requisites

Interested in a detailed, practical introduction to clinical quantitative LCMS Overview

Is your laboratory under pressure to purchase an LC-tandem MS or is the ROI you wrote last year haunting you now? This short course is designed for attendees implementing quantitative LC-tandem MS for patient testing who have laboratory medicine experience but no mass spectrometry training - CLS bench analysts, supervisors, R&D scientists, and laboratory directors. Theoretical concepts necessary for a robust implementation of clinical mass spectrometry will be presented – but the emphasis is on practical recommendations for:

  1. LC-MS/MS system purchasing
  2. site preparation and installation
  3. defining preliminary MSMS and LC parameters for your first method
  4. selecting and optimizing sample preparation for your first method
  5. choosing internal standards, solvents, and water, making reagents and calibrators
  6. adjusting sample preparation, LC and MSMS parameters to achieve the desired assay performance
  7. establishing data analysis & review criteria and an interface to the LIS
  8. pre-validation stress testing and method validation
  9. preventative maintenance and troubleshooting
  10. maintaining quality in production
2074
Sunday
1830
2030
Welcome Dinner Buffet
@ Ferrantes (10th Floor @ Marriott)
2045
Sunday
2030
2200
Hospitality Lounge
@ Ferrantes (10th Floor @ Marriott)
2091

Monday

Monday
645
800
Short Course Breakfast Buffet
@ Portola Club Room

Full Breakfast Buffet provided for Short Course attendees.
2101
Monday
700
1800
Registration Desk Open
@ Serra Foyer
2081
Short Course : LC-MSMS 101 : Getting Started with Quantitative LC-MSMS in the Diagnostic Laboratory
@ De Anza 3

IN-PERSON & ON-LINE

Course Schedule

Segment 1 : Sunday 17:00 - 18:30 (1.5 h)
Segment 2 : Monday 08:00 - 12:00 (4 h)
Segment 3 : Monday 13:30 - 17:30 (4 h)
Segment 4 : Tuesday 08:00 - 12:00 (4 h)
Segment 5 : Tuesday 13:30 - 16:00 (2.5 h)

Total Contact Hours: 16.00

2074
Short Course : LC-MSMS 201 : LC-MSMS Technology and Techniques in the Clinical Lab
@ De Anza 1

Robert Voyksner, PhD
LCMS Limited


Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

----------

Summary

This course is designed for the chromatographer and /or mass spectrometrist who wants to be successful in developing methods, optimizing methods, troubleshooting methods and solving problems using LC-MSMS. The course covers the atmospheric pressure ionization (API) techniques of electrospray and gas phase ionization including atmospheric pressure chemical ionization (APCI) and atmospheric pressure photo ionization (APPI) using triple quadrupole, time-of-flight and quadrupole time of flight and orbit trap mass analyzers. Discussions of sample preparation and modes of chromatography will target method development and optimization for the analysis of “real-world” samples by LC/MS. The course highlights the following topics with respect to development and optimization of methods to achieve the best sensitivity, specificity, and sample throughput.

This course focuses on method development method trouble shooting and application for the analysis of both small and large molecules that are clinically relevant. All examples are taken from real-world analyses, performed by Dr. Voyksner at LCMS Limited. The concepts presented in the course are reinforced through numerous problem sets the attendees will work on throughout the 12 hour course.

The last part of the course is an open forum where each attendee is invited to share a current LC-MS/MS issue they face. As a class we work through potential solutions and experiments to be performed to find a solution to the student problem, applying the concepts taught in the class and Dr. Voyksner’s 40 plus years of experience in LC-MS/MS. From past classes this has been the attendee’s favorite part of the class.

Specific topics to be covered include

  1. Understanding API ionization processes for electrospray, APCI and APPI, what affects the ionization process and how to maximize the ionization for compounds of interest.
  2. Understanding the effects of LC columns (dimensions and particles size), flow rate, and mobile phases have upon the separation and LC/MS analysis.
  3. Determining the type of ions that can form by electrospray and APCI, how to interpret the MS and MS/MS spectra and approaches on how to perform qualitative analysis in LC-MS/MS and high-resolution MS/MS.
  4. Understanding important issues that affect quantitative analytical results and how to optimize the method to achieve the best performance, reduce matrix suppression, reduce background and generate the best accuracy and precision.
  5. Exploring what new techniques are available (e.g. direct analysis MS, chip method and MS instrumentation) that can improve the results one can obtain.
  6. Open forum discussing attendees’ specific problems they face in method development or analysis using LC-MS/MS.
2069
Short Course : Data Science 101 : Breaking up with Excel: An Introduction to the R Statistical Programming Language
@ Steinbeck 1

Daniel Holmes, MD, FRCPC
St. Paul’s Hospital

Dustin Bunch, PhD, DABCC
Nationwide Children's Hospital


Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

Segment 1 is also be available ONLINE (pre-recorded) if you can't make it in person.

----------

Does Excel lag on you when you open a file bigger than 1000 rows? Has it ever changed your data to a date against your will? Are you ready to jump right past Tableau and into the world of Data Science using a real programming language?

Well, your wait is over because at MSACL we again will be offering a course for complete programming newbies that will help you get going analyzing real data related to LC-MS/MS assay development, validation, implementation and publication.

The only background expected is the ability to use a spreadsheet program. The skills that you will acquire will allow you to take advantage of the many tools already available in the R language and thereafter, when you see that your spreadsheet program does not have the capabilities to do what you need, you will no longer have to burst into tears.

The course will be run over two days and time will be evenly split between didactic sessions and hands on problem solving with real data sets. Drs Holmes and Bunch will adopt a “no student left behind policy”. Students will be given ample time to solve mini problems taken from real life laboratory work and focused on common laboratory tasks. All attendees will need to bring a laptop with the R language installed R Studio interface installed. Students may use Windows, Mac OSX or Linux environments. Both R and R studio are free and open-source. No cash required.

Students should be prepared for learning what computer programming is really like. This may involve some personal frustration but it will be worth it.

Obtaining the Software

!!! DOWNLOAD PROGRAM PACKAGES PRIOR TO ARRIVAL ONSITE !!! THERE WILL NOT BE OPEN INTERNET WIFI IN THE CONFERENCE CENTER.

!!! POWER : Make sure your computer is charged to hold power for 4-8 hrs, as power outlets may not be available.

Instructions for installing the R language are here: http://cran.r-project.org/
Instructions for installing R Studio are here: http://www.rstudio.com/

Course Description

The course will cover:

  1. The major types of R variables: vectors (numerical, character, logical), matrices, data frames and lists.
  2. The important classes: numeric, character, list and changing between them
  3. Importing data from Excel
  4. Dealing with non-numeric instrument data
  5. Manipulating and cleansing your data
  6. Exporting data to Excel-like format.
  7. Basics of tidyverse: dplyr, filter, mutate, join
  8. Regressions: ordinary least squares,Passing Bablok, Deming, weighted regressions.
  9. Non-linear regressions
  10. Looping: Doing things repeatedly
  11. group_by and summarize
  12. Writing your own functions
  13. Making highly customized figures with base plot or ggplot
  14. Putting it all together projects:
  15. Preparing method comparison regression and Bland Altman plots
  16. Preparing mass spectrometry data for upload to LIS.
2050
Short Course : Sample Prep 201 : Sample Preparation and Alternative Matrices for LC-MS Assays
@ De Anza 2

William Clarke, PhD, MBA, DABCC
Johns Hopkins University School of Medicine

Mark Marzinke, PhD, DABCC, FAACC
Johns Hopkins University School of Medicine


Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

----------

Summary:

This course will encompass various sample preparation approaches used for LC-MS assays. The course will highlight not only the importance of sample processing in the clinical laboratory environment, but also illustrate the “fit for purpose” application of processing techniques in clinical mass spectrometry. This course will also discuss the theory behind different specimen preparation methods, strengths and weaknesses of each approach, as well as opportunities for automation. The first section of the course will serve as a primer of the role of upfront sample management, utilizing examples in blood and urine specimen sources. There will also be an introduction to the application of LC-MS approaches in alternative matrices. The second section of the course will elaborate on the foundations established in the first half, and expand into newer technologies and automated alternatives for sample processing. Topics will be covered through lecture, Q&A, Case Studies, and small group exercises.

Topics covered include

  • Pain points in clinical LC-MS
  • Overview of specimen processing in laboratory medicine
  • Off-line and On-line sample processing
  • Analysis of blood and urine
  • Alternate body fluid specimens (e.g. CSF, breast milk, tissue, etc.)
  • Dried specimens as matrices
  • Automation of sample processing
Learning Objectives

After attending this short course, participants will be able to:

  1. Describe various pain points and challenges in clinical LC-MS;
  2. Discuss the impact of various specimen preparation approaches on LC-MS assay performance;
  3. Implement a fit-for-purpose approach to selection of a specimen preparation approach in their laboratory practice;
  4. Describe alternative specimen types and their potential utility in clinical practice or research.
2059
Short Course : Clinical Proteomics 201 : Clinical Proteomics
@ Steinbeck 3

Andy Hoofnagle, MD, PhD
University of Washington

Christopher Shuford, PhD
Labcorp

Cory Bystrom, PhD
Ultragenyx


Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

----------

Objective

The main goal of this course is to provide an interactive forum in which attendees will be introduced to critical aspects of clinical protein measurements. The topics of this course will be templated on the framework of CLIS guidance document, C64: Quantitative Measurement of Proteins and Peptides by Mass Spectrometry.

Summary

The motivation for using mass spectrometry to quantify proteins in clinical research and in clinical care will be discussed as part of this interactive workshop. Technical topics uniquely affecting quantitative protein and peptides measurements by mass spectrometry will be a point of emphasis. Case studies from assay inception through validation will be presented and participants will work interactively to critique various aspects of clinical proteomic measurements.

Syllabus

  1. Protein vs Peptide Measurands
  2. Workflows
  3. Sample Preparation (Digestion & Enrichment)
  4. Internal standards
  5. Calibration
  6. Validation
  7. Quality control
2066
Short Course : GlycoProteomics 101 : Clinical glyco(proteo)mics by mass spectrometry
@ Stevenson 2

Noortje de Haan, PhD
University of Copenhagen

Constantin Blöchl, PhD
Center for Proteomics and Metabolomics, Leiden University Medical Center, The Netherlands


Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

----------

Summary:

Did you ever encounter glycans, but you -kind of- neglected them as they seemed too complicated to characterize? Or did you just perform a glycan release to make the analysis of your protein a lot easier? You have no idea how to interpret your data when a glycan is present? Fear no more! We are here to provide you with the basics in the field of mass spectrometric glycomics and glycoproteomics.

The course will start with a historical overview on glycan research (i.e. how did glycans work their way up to being acknowledged as important study objects) and we will guide you through the maze of different nomenclatures. Moreover, although glycans are well known for their complexity, we will reveal to you the “rules of glycan structures” based on known biosynthetic pathways. This will be followed by an in-depth discussion on glyco(proteo)mic mass spectrometric technologies and workflows. In addition, different sample preparation steps and data analysis approaches will be covered. We will close-up with a session about glycomic biomarker discovery.

The course will run over two days and time will be split between lectures and workshops (e.g. how do you recognize a glycan in a mass spectrum and how do you assign it). While not everything can be covered within these two days we will ensure that you will know your “glyco-basics” in the end. Moreover, participants are encouraged to submit any specific glyco-questions they have prior to the course and we will try to discuss them during the course.

Objectives:

  1. Understand glycan nomenclature and biosynthesis to aid mass spectrometry data interpretation.
  2. Know what analytical method to choose for you specific glycomics experiment.
  3. Learn how to interpret MS1 and MS2 data of glycans and glycopeptides.
  4. Know what software to use to aid glyco(proteo)mics MS data processing.
2053
Monday
1200
1330
Lunch
@ Off-site

Short Course attendees receive complimentary lunch at Dustbowl Brewery. Receive voucher from instructor or at registration desk.

Other Recommended Places to Eat

2099
Short Course : LC-MSMS 101 : Getting Started with Quantitative LC-MSMS in the Diagnostic Laboratory
@ De Anza 3

IN-PERSON & ON-LINE

Course Schedule

Segment 1 : Sunday 17:00 - 18:30 (1.5 h)
Segment 2 : Monday 08:00 - 12:00 (4 h)
Segment 3 : Monday 13:30 - 17:30 (4 h)
Segment 4 : Tuesday 08:00 - 12:00 (4 h)
Segment 5 : Tuesday 13:30 - 16:00 (2.5 h)

Total Contact Hours: 16.00

2074
Short Course : Data Science 101 : Breaking up with Excel: An Introduction to the R Statistical Programming Language
@ Steinbeck 1

Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

2050
Short Course : Data Science 201 : Flexing with R : Databases to Dashboards
@ Colton

Shannon Haymond, PhD
Northwestern University Feinberg School of Medicine

Patrick Mathias, MD, PhD
University of Washington


Course Schedule

Segment 1 : Monday 13:30 - 17:30 (4 h)
Segment 2 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 8.00

------------

Pre-requisites

An introductory R course (including MSACL Data Science 101) and/or experience using R for data analysis

Objectives

To teach learners with a basic background with R how to organize data analysis projects reproducibly using tools such as Quarto, dashboards, and databases.

Summary

Do you have data files that you would like to accumulate over time into an organized, accessible format and then visualize different aspects of the combined data in an interactive, web-based dashboard? If so, this course is for you! Reproducibility is an important principle for making data analysis trustworthy and reliable. Automation enables users to scale their data analysis steps. The R programming language is one of many tools that can help users automate data analysis workflows while adopting best practices in reproducibility, but there are several packages to choose from when developing these skills. In this short course we will introduce a combination of workflows, packages, and tools that help learners set up data analysis projects, develop pipelines for extracting and storing data, and then develop interactive visualizations to gain understanding from the data. First, we will explain how to fully utilize the RStudio integrated development environment with Projects and how the renv package ensures that code consistently produces the same output no matter where or when it is run. Next, we will cover iterative file parsing and demonstrate how this can be linked to lightweight relational databases such as SQLite and DuckDB to build a pipeline for repetitive data loading over time. In the last portion of the course, we will discuss the Quarto scientific publishing system and related visualization tools such as the flexdashboard package. This short course will be interactive, with frequent short exercises to reinforce new concepts. Familiarity with the R programming language, either from an introductory course or self-learning, is highly recommended. Finally, concepts in this short course overlap material taught in previous intermediate R courses at MSACL, but here we will focus putting together the tools to develop reproducible, automated dashboards for visualization of laboratory data and provide updates to include some of the latest developments in the R ecosystem.

Syllabus / Topics covered

  1. Principles of reproducibility
  2. Pick up where you left off with RStudio tools
  3. Taking control of packages with renv
  4. File reading over and over and over
  5. Dude, where’s my data? Organizing data into relational databases
  6. Pulling out your dplyr to grab what you need
  7. Show off your skills with Quarto
  8. Flexing with some sweet dashboards
2063
Short Course : Sample Prep 201 : Sample Preparation and Alternative Matrices for LC-MS Assays
@ De Anza 2

Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

2059
Short Course : Clinical Proteomics 201 : Clinical Proteomics
@ Steinbeck 3

Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

2066
Short Course : Metabolomics 201 : Measuring Metabolism from Dried Blood Spots to Microsampling and more
@ Steinbeck 2

Tim Garrett, PhD
University of Florida College of Medicine

Donald Chace, PhD, MSFS, FACB
Capitainer


Course Schedule

Segment 1 : Monday 13:30 - 17:30 (4 h)
Segment 2 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 8.00

------------

Objectives

(1) Advantages for using DBS and other microsampling techniques in clinical and metabolomics research, discovery, and applications,

(2) Strategies for maximizing analytical success with the mass spectrometer and DBS with examples of existing methods and successful applications.

(3) Understand the application of global metabolomics and targeted metabolomics to disease diagnostics.

Summary

DBS have been used for nearly 60 years in a clinical and research environment specifically in rare disease screening of newborns or health monitoring and treatment to restore a more “normal” metabolism. Although clinical chemistry workflows are still dominated by liquid blood or plasma and immunoassay platforms, they are not necessarily suitable for microsample collection as demonstrated in the choice for newborn screening (200-300 µL) versus 1-10 mL for a venous blood draw. Furthermore, a dried microsample offers better protection from infectious disease during sample handling, is less expensive to ship in the mail, may stabilize some molecules from degradation of active enzyme, light or heat, and has reduce storage requirements during and after sampling. Perhaps the biggest advantage that is now recognized in the “post” pandemic world is remote patient sampling in an ever-expanding telemedicine environment. This course will describe the advantages of filter paper for mass spec workflows in areas of sample cleanup, extraction, manipulation as well as examples of successful analysis. We will provide examples of existing method in use in clinical analysis. As important are its advantages, we will discuss limitations from the lack of precision of classic Guthrie cards because of volume uncertainties to the problems of some mass spectrometry analysis of molecules like proteins. Finally we will correlate these issues with the ever expanding area of metabolomics, how research might benefit from small microsamples and remote sample collection especially on diseases that are rare in a patient that might be a continent away.

Topics covered

  1. Metabolomics/Metabolism
    • Global analysis (HRMS, HRMS/MS)
    • Targeted analysis (SRM, MRM, NL, PS)
    • Rare disease diagnosis
  2. Dried blood spots/microsampling
    • Collection
    • Extraction
    • Quantitation
    • Comparisons to other matrices (plasma/serum, urine, whole blood)
    • Tips/tricks
  3. Future perspectives
    • New collection devices
    • New approaches
    • Standardization/QA/QC
    • Precision/telemedicine
2061
Short Course : GlycoProteomics 101 : Clinical glyco(proteo)mics by mass spectrometry
@ Stevenson 2

Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

2053
Short Course : LC-MSMS 201 : LC-MSMS Technology and Techniques in the Clinical Lab
@ De Anza 1

Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

2069
Monday
1730
1830
Happy Hour
@ Steinbeck Foyer
1991
Monday
1830
2000
Dinner On Own
@ Off-site
1990
Monday
2000
2400
MSACL Hospitality Lounge
@ Portola Club Room

Drinks, snacks, foosball and open mic!
1989

Tuesday

Tuesday
645
800
Short Course Breakfast Buffet
@ Portola Club Room

Full Breakfast Buffet provided for Short Course attendees. You are going to need the hour to eat, so get there on time.
2082
Tuesday
700
1800
Registration Desk Open
@ Serra Foyer
2092
Short Course : LC-MSMS 101 : Getting Started with Quantitative LC-MSMS in the Diagnostic Laboratory
@ De Anza 3

IN-PERSON & ON-LINE

Course Schedule

Segment 1 : Sunday 17:00 - 18:30 (1.5 h)
Segment 2 : Monday 08:00 - 12:00 (4 h)
Segment 3 : Monday 13:30 - 17:30 (4 h)
Segment 4 : Tuesday 08:00 - 12:00 (4 h)
Segment 5 : Tuesday 13:30 - 16:00 (2.5 h)

Total Contact Hours: 16.00

2074
Short Course : Data Science 101 : Breaking up with Excel: An Introduction to the R Statistical Programming Language
@ Steinbeck 1

Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

2050
Short Course : Data Science 201 : Flexing with R : Databases to Dashboards
@ Colton

Course Schedule

Segment 1 : Monday 13:30 - 17:30 (4 h)
Segment 2 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 8.00

2063
Short Course : Sample Prep 201 : Sample Preparation and Alternative Matrices for LC-MS Assays
@ De Anza 2

William Clarke, PhD, MBA, DABCC
Johns Hopkins University School of Medicine

Mark Marzinke, PhD, DABCC, FAACC
Johns Hopkins University School of Medicine


Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

2059
Short Course : Clinical Proteomics 201 : Clinical Proteomics
@ Steinbeck 3

Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

2066
Short Course : Metabolomics 201 : Measuring Metabolism from Dried Blood Spots to Microsampling and more
@ Steinbeck 2

Course Schedule

Segment 1 : Monday 13:30 - 17:30 (4 h)
Segment 2 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 8.00

2061
Short Course : GlycoProteomics 101 : Clinical glyco(proteo)mics by mass spectrometry
@ Stevenson 2

Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

2053
Short Course : LC-MSMS 201 : LC-MSMS Technology and Techniques in the Clinical Lab
@ De Anza 1

Course Schedule

Segment 1 : Monday 08:00 - 12:00 (4 h)
Segment 2 : Monday 13:30 - 17:30 (4 h)
Segment 3 : Tuesday 08:00 - 12:00 (4 h)

Total Contact Hours: 12.00

2069
Tuesday
1200
1330
Lunch
@ Off-site

Short Course attendees receive complimentary lunch at Dustbowl Brewery. Receive voucher from instructor or at registration desk.

Other Recommended Places to Eat

2100
Short Course : LC-MSMS 101 : Getting Started with Quantitative LC-MSMS in the Diagnostic Laboratory
@ De Anza 3

IN-PERSON & ON-LINE

Course Schedule

Segment 1 : Sunday 17:00 - 18:30 (1.5 h)
Segment 2 : Monday 08:00 - 12:00 (4 h)
Segment 3 : Monday 13:30 - 17:30 (4 h)
Segment 4 : Tuesday 08:00 - 12:00 (4 h)
Segment 5 : Tuesday 13:30 - 16:00 (2.5 h)

Total Contact Hours: 16.00

NOTE: THIS occurs AT THE SAME TIME as the WORKSHOPS.

2074
Tuesday
1330
1600
Workshop : Surgical Mass Spectrometry - Delivering the Technology to the Operating Room
@ Steinbeck 1

Zoltan Takats, PhD
Imperial College


Objectives

The objective of the workshop is to identify and overcome potential roadblocks preventing mass spectrometry-guided surgery to become routinely used in the clinical interventional world. The workshop will focus on the side-by-side comparison of existing technology and clinical needs, as also on the embedding of the technology into existing healthcare settings and regulatory aspects. The workshop is envisioned to outline the strategy for the clinical translation of these technologies.

Summary

The need for in-situ, real-time tissue identification has been dramatically increasing with the development and deployment of robotic and other high-precision surgical approaches. While surgical mass spectrometry techniques have been continuously developed, published and demonstrated in human surgical environment, none of these approaches have reached regulatory approval and routine application in surgery. We are planning to use the meeting to understand where the roadblocks are and how we can get around them to deliver the technology for patients and healthcare professionals equally needing it. The workshop is envisioned to map out the current technology landscape and identify the strengths and weaknesses of each solution together with their respective low-hanging fruit applications as the first main topic. This mapping exercise will be followed by discussing the embedding of the approach both into existing oncology and clinical diagnostic systems. The approach is expected to change how interventional cancer care (and potentially a few other treatments) is delivered, hence it is of utmost importance to understand this aspect from the point of view of pathologists, oncologists and medical imaging professionals. Furthermore, the technology will also be the forerunner of a universal, mass spectrometry-based diagnostic system, bringing LC-MS and MS imaging experts to the table. Beyond the embedding of the technology, we are also keen to discuss the regulatory and health economic aspects of the approach in the third part of the workshop.

Syllabus/Topics

  • Surgical mass spectrometry methods – strengths, weaknesses, applications and future perspectives
  • Embedding of technology into healthcare systems. Interactions with oncology, pathology, imaging and clinical chemistry. Synergism with other MS-based diagnostic technologies.
  • Regulatory and health economics aspects
2090
Tuesday
1330
1600
Workshop : Design of Experiments for Optimization of LC-MS Clinical Assays
@ Steinbeck 2

Margret Thorsteinsdottir, PhD
University of Iceland

Finnur Eiriksson, PhD
ArticMass


Objectives

The objective of the workshop is to provide an introduction into design of experiments (DoE) for clinical application with special focus on optimization of MS-based clinical assays. The workshop is focused on practical implementation of DoE and will demonstrate how method development of sample preparation and UPLC-MS/MS method for quantification of clinical biomarkers can become much more efficient by utilizing DoE.

Summary

Design of experiments (DoE) is an efficient tool for development and optimization of UPLC-MS/MS platform for quantification of biomarkers in complex biological matrices. The UPLC-MS/MS platform is composed of several processes which involve many experimental factors that need to be simultaneously optimized to obtain a true maximum sensitivity with adequate resolution at minimum retention time. DoE offers a practical approach for performing experiments in accordance with a predefined plan, modelling by empirical functions, and graphical visualization. Basic concept of DoE will be presented with emphasis on practical implementation of DoE which includes the three main stages, screening, optimization, and robustness testing. To demonstrate the cost-effective benefit of DoE, which allows the effect of variables to be assessed with only a fraction of the experiments that would be required by changing one-separate-factor-at-time (COST) approach, two case studies will be presented. The first case is optimization of sample preparation in bottom-up targeted protein LC-MS workflow using DoE. The second case is an optimization of a UPLC-MS/MS assay for clinical diagnostic and therapeutic drug monitoring of patients with adenine phosphoribosyltransferase (APRT) deficiency, which is an inborn error of purine metabolism. A polynomial model which corresponds to the objective of the case study is specified and an experimental design that supports the selected model is generated. Significant factors were studied via central composite design and related to responses utilizing partial least square (PLS)-regression. Both cases showed that DoE is an excellent tool for optimization of sample preparation for biological samples and UPLC-MS/MS quantification method for clinical biomarkers. A significant reduction of sample preparation time was achieved with increased yields for selected peptides and a reliable UPLC-MS/MS assay for simultaneous quantification of urinary 2,8-dihydroxyadenine (DHA) and adenine was optimized efficiently with DoE.

Syllabus

  • Design of Experiments (DoE) – Get it right from the beginning
  • Basic concept and assessment of DoE
  • Optimization of sample preparation and UPLC-MS/MS clinical assay by DoE
1995
Tuesday
1330
1600
Workshop : Ion Mobility: How to Get it into YOUR Lab
@ Steinbeck 3

Christopher Chouinard, PhD
Clemson University

Robin Kemperman, PhD
Children’s Hospital of Philadelphia


Objective

Attendees will learn the basic principles of ion mobility, benefits and challenges to routine implementation in the clinical lab, method development, and current applications.

Summary

Ion mobility-mass spectrometry (IM-MS) has become a cornerstone of biomedical analysis, with applications ranging from isomeric small molecule differentiation to the study of protein structure. Despite its advantages, IM-MS has yet to see routine implementation in the clinical lab due to challenges in quantitation, limited universal standards, data processing software, and reproducibility across different IM techniques/vendor platforms. This workshop will introduce common IM techniques and their operating principles, expanding upon the benefits of incorporating IM into conventional LC-MS/MS workflows and discussing its challenges. A discussion of method design and development will focus on how users might go about integrating IM into their existing (or new) assays. Finally, an overview of current applications (including metabolomics, lipidomics, and proteomics examples) will be provided.

Objective 1: Understand the basic operating principles of IMS and the differences between the different techniques (e.g., drift tube, traveling wave, FAIMS/DMS, etc.)
Objective 2: Recognize the benefits and limitations to incorporating IMS into conventional LC-MS/MS workflows in the clinic
Objective 3: Become familiar with method design and development and current/future applications

Syllabus

  • Basic Operating Conditions of IMS: Electric field application, experimental conditions (temperature, pressure, gas composition)
  • Different IMS techniques: Drift tube/traveling wave, field asymmetric/differential mobility, emerging techniques (i.e., TIMS, SLIM, cIMS, etc.)
  • Applications: Current examples from metabolomics, lipidomics, and proteomics
2079
Tuesday
1330
1600
Workshop : The Value, Impact, and Regulatory Landscape of Laboratory Developed Tests
@ Colton

Melissa Budelier, PhD
TriCore Reference Laboratories

Jacqueline Hubbard, PhD, DABCC
Three Rivers Diagnostics


Objectives

  • Recognize the role of laboratory developed tests (LDTs) in patient care
  • Describe the current regulatory landscape of LDTs
  • Define the VALID act and discuss its potential implications to the practice of laboratory medicine and patient care

Summary

Laboratory Developed Tests (LDTs) play a critical role in patient care. They are developed by expert laboratorians to bridge gaps between patient needs and available FDA-approved assays. LDTs are used to help diagnose and manage treatment for a variety of health conditions. These tests undergo extensive validation prior to implementation and, within the United States, are federally regulated by CLIA. The proposed Verifying Accurate Leading-edge IVCT Development Act of 2022 (“VALID Act”) includes additional regulation of LDTs by the FDA. If passed, the VALID Act would limit LDT access and the ability to provide timely laboratory testing to providers and patients, resulting in significant care gaps.

This interactive workshop will provide an overview on what LDTs are, how they are validated and the current regulatory framework, highlighting papers published in the recent JMSACL special issue – Laboratory Developed Tests: Regulation, Assay Development, and Impact on Patient Care. We’ll also provide an overview on the status and potential implications of the VALID act. In the second half of the workshop, the audience will have a chance to share their opinions, questions, and concerns in a flipped classroom Q & A.

2080
Tuesday
1330
1600
Workshop : Pre-Analytical Considerations as Prerequisite for Successful Clinical Application of Lipidomics
@ Stevenson 2

Robert Gurke, PhD
Fraunhofer Institute for Translational Medicine and Pharmacology ITMP

Anne K. Bendt, PhD
Singapore Lipidomics Incubator (SLING), National University of Singapore

Bo Burla, PhD
Sling @ National University of Singapore


Objectives

It is the objective of the workshop to provide an introduction into pre-analytical considerations for clinical application of MS-based analytics, with a special focus on lipids. However, these considerations are in many cases independent of specific analytes of interest and can hence be applied for polar metabolites as well as proteins. The workshop is focused on main hurdles to be considered when performing clinical research using LC-MS based determination of lipids namely: (I) pre-analytical sample handling, (II) frequent generation of patient samples as well as (III) overall cohort and study design.

Summary

Lipids are involved in a broad spectrum of functionalities in the organism and implicated in a variety of physiological and pathological processes. Changes in lipid levels are promising biomarkers for early diagnosis, prognosis of disease progression, or guidance for selecting promising therapeutic approaches. However, biomarker discovery in the field of lipidomics is a very challenging process since lipids require specific procedures regarding sampling (including selection of sample type and collection procedures, storage conditions and number of freeze-thaw cycles), sample preparation and analysis for reliable determination. As especially pre-analytical sample handling is a critical step for the reliable analysis of the lipidome, a close cooperation with the clinicians is necessary. This ensures following a highly standardized protocol for venous blood sampling to avoid several pre-analytical pitfalls potentially changing the lipid profile ex vivo. As collecting venous blood samples is very laborious for patients as well as clinicians, other ways for a more frequent sample collection are necessary. Therefore, capillary blood sampling using microsampling devices is an auspicious way to complement the strategy of analyzing venous blood-based samples taken in the clinics. Besides considering the right sampling strategy, the structured recording of sampling details and subject metadata, the overall planning of the study and also the cohorts to be included is of high relevance. All mentioned points have to be considered before starting a clinical research project applying lipidomics to generate high quality data making biomarker discovery possible.

2078
Tuesday
1330
1600
Workshop : Setting up your first LC-MS/MS assay (from Start to Finish)
@ De Anza 1

Joshua Hayden, PhD, DABCC, FACB
Norton Healthcare


Objective

The objective of this workshop is to walk attendees through the process of acquiring/installing an instrument, developing their first assay, validating the assay as a laboratory developed test, and then ensuring adequate performance once live. This will be done using the presenters experience implementing a liquid chromatography tandem mass spectrometry (LC-MS/MS) assay to quantify buprenorphine in human urine. At the end of the workshop, attendees should be able to:

  1. Justify the acquisition of an LC-MS/MS for clinical use
  2. Explain the major steps involved in developing a small molecule LC-MS/MS assay
  3. List the required elements for clinical validation of a small molecule LC-MS/MS assay
  4. Propose the steps necessary to ensure accurate results once live on a validated LC-MS/MS method

Summary The continuing expansion of mass spectrometry into the clinical laboratory involves both expanding what testing can and should be done by mass spectrometry as well as expanding the number of labs implementing and utilizing existing/established assays. Unfortunately, too often the barrier of entry into mass spectrometry can be quite high. This workshop is designed to help walk attendees through the process of acquiring/installing an instrument, developing their first assay, validating the assay as a laboratory developed test, and then ensuring adequate performance once live (post-implementation monitoring). In particular, attendees will be given an overview of how these steps were done when the presenter setup and implemented a liquid chromatography tandem mass spectrometry (LC-MS/MS) assay to quantify buprenorphine in human urine in a College of American Pathologists (CAP) accredited laboratory. Special attention will be given resources that are available to assist labs undertaking the development and validation of clinical LC-MS/MS assays, resources such as published papers and CLSI guidelines. Practical factors such as sourcing consumables and necessary operating procedures will also be included. While the focus will be on implementing this specific assay, the goal is to share general best practices that were used that can be applicable to other assays.

Syllabus / Topics Covered

Presenting your business case and installing your instrument (0.5 hours, with intro)

Developing your assay (0.75 hours)
-Setting up chromatography
-Optimizing signal
-Selecting consumables (internal standards, QC, calibrators, mobile phase, etc)
-Finding your pitfalls

Validating your assay (0.75 hours)
-Reportable range
-Accuracy
-Precision
-Ion suppression
-Stability
-Testing your pitfalls

Post-implementation monitoring (0.5 hours)
-Daily quality assurance (SST, blanks, QC, etc)
-Data review
-Training/competency assessments

2127
Tuesday
1600
1630
Coffee Break
@ Steinbeck Foyer
1997
Tuesday
1630
1650
Welcome & Scientific Orientation
@ Steinbeck Ballroom

Kara Lynch, PhD, DABCC
University of California San Francisco

1996
Tuesday
1650
1740
The Michael S. Bereman Award for Innovative Clinical Proteomics : Translating Multiplexed Proteomic Assays to the Clinic and Beyond: Lessons from a Road Less Traveled
@ Steinbeck Ballroom

Timothy Collier, PhD
Quest Diagnostics

Sponsored by:


MICHAEL S. BEREMAN AWARD LECTURE

The process of translating mass spectrometry (MS)-based proteomic assays from basic research to the clinical laboratory remains a significant challenge for many laboratorians. The road to using innovative assays to aid in patient treatment is often fraught with obstacles, be they technical, financial, or regulatory. Over the past several years, our laboratory has had success in the research and development, validation, and commercialization of a multi-marker assay of high-density lipoprotein (HDL)–associated proteins. The validated clinical assay provides insight into a patient’s cholesterol efflux capacity (the ability of HDL cholesterol to transport cholesterol away from the artery wall). The assay helps assess the patient’s risk for developing coronary artery disease and, ultimately, cardiovascular death. The assay is also a component in several clinical studies to further assess its utility. Furthermore, the research framework upon which this assay was designed continues to yield new insights into other pathologies, including non-alcoholic fatty liver disease (NAFLD), metabolic syndrome, and diabetes. In this presentation I will summarize our efforts, our successes, and lessons learned on the road from basic research to clinical deployment.

Moderated by:

Mari DeMarco, PhD, DABCC, FACB, FCACB
University of British Columbia

1998
Tuesday
1740
1830
Distinguished Contribution Award : The Next Phase of Precision Medicine
@ Steinbeck Ballroom

Jennifer Van Eyk, PhD
Cedars-Sinai Heart Institute

The Award Medal
(100% metal)


On the Award.

Underlying precision medicine is the concept that an individual’s Omic signature (including the proteome) will provide a physician with clinically actionable diagnosis and a subsequent mechanistic therapeutic route. This requires i) having an array of mechanistic therapies for each disease and ii) a means to diagnosis (identify) which therapy (or combination) will be appropriate for a particular person. Proteomics has played a role in discovery and defining potential mechanistic routes, but it is now time to move into implementation. This will, we hope, drive democratize of precision medicine.

Moderated by:

Ravinder Singh, PhD
Mayo Clinic

Gwen McMillin, PhD
University of Utah and ARUP

2075
Tuesday
1830
2100
Opening Exhibits Reception
@ Exhibit Hall - Serra
1999
2000
2045
Troubleshooting Posters
@ Exhibit Hall - Serra

To be held in the Troubleshooting Poster Presentation Corner (lower left) of the Exhibit Hall.
20:00 @ poster #30a
The Importance of Using Isotopically Matched Internal Standards in Liquid Chromatography-mass Spectrometry-based Assays to Minimize Matrix Effects
Karen Bolhuis
Corewell Health Hospitals
20:30 @ poster #31a
In Vitro Conversion of CBD-COOH to THC-COOH during Sample Preparation
Katherine Turner
Corewell Health
Tuesday
2100
2400
MSACL Lounge
@ Portola Club Room

Drinks provided. Plus foosball and open mic!
2046

Wednesday

Wednesday
600
645
Sunrise Tai Chi / Qigong Activity
@ Ferrantes (10th Floor @ Marriott)

Tai Chi and Qigong (pronounced chee-gong) explore the underlying internal practices and approach to fitness training beyond merely moving the body. Ideal for stress reduction this experience involves the interpenetration of mind and body during movement; meaning it can’t just be the mind, or just the body separately. Qigong exercise includes opening energy centers, joints, and tendons. This is achieved in part through abdominal breathing to calm the mind and in turn relax the body in movement. During the relaxation phase you will learn to activate and sense energy kinesthetically in “felt” terms through simple moving practices. Practice session includes Healing Sounds which function in discharging heat and toxins trapped within the fascia around the internal organs. Qigong exercises together with Tai Chi aim to produce a mental and physical sense of balance, centeredness, and harmony.

What to wear: One principle of Tai Chi and Qigong is relaxed, unrestricted movement, therefore casual loose-fitting clothing is recommended. Avoid tight fitting clothing. Skin breathes, body moves freely with more mobility. Remove any belts during practice. Footwear: Flat soled shoes preferable to feel balanced and rooted or connected to the floor, as opposed to high arched athletic shoes or raised heels. Shoes off OK as long as you aren’t subject to easily slipping or sliding.

2083
Wednesday
700
800

Breakfast Industry Workshop(s)

Thermo Fisher Scientific
@ Steinbeck 1

Promising Clinical Applications of ICP-MS

No child lead behind: Development of a method for lead screening using dried blood spots
Jessica Colón-Franco, PhD
Cleveland Clinic Foundation
Advancing ICP-MS to clinical testing: A novel highly sensitive diagnostic platform for early detection and therapeutic monitoring of bone metabolic disorders
Michael Lutz, PhD
Osteolabs GmbH

Indigo BioAutomation
@ Steinbeck 2

Pre-Register

Laboratory Analysis as a Team Sport

Part I – Prepare, Practice, Perform
Ridhima Rao, MS - Forensic Sciences
Millennium Health
Part II – Decision Support and Analytics
Jim Edwards
Indigo BioAutomation

Waters
@ Steinbeck 3

Pre-Register

The Evolving Power of LC-MS/MS Technology

The Evolving Power of LC-MS/MS Technology within Endocrinology
Dominic Foley, BSc
Waters Corporation
The Evolving Power of LC-MS/MS Technology within Endocrinology
David Ballantyne
Waters Corporation
Low-Res, High-Sens Proteomics: Applications and Obstacles
Dennis Orton, PhD, FCACB
Alberta Precision Labs University of Calgary

Wednesday
800
845
Coffee Break
@ Exhibit Hall - Serra

Visit the Exhibit Hall for a relaxed tour of the posters and Exhibits while enjoying your morning coffee.
2087
Wednesday
800
845
Meet-a-Mentor Booth Tour
@ Exhibit Hall - Serra

Join Laura Sanchez for a tour of posters the Exhibit Hall while enjoying your morning coffee.
2123
Wednesday
800
845
Coffee Roundtables
@ Steinbeck Foyer

Margret Thorsteinsdottir, PhD
University of Iceland

Finnur Eiriksson, PhD
ArticMass

Hsuan-Chieh (Joyce) Liao, PhD, NRCC, DABCC
University of Washington

Liang Li, PhD
Metabolomics Innovation Centre of Canada & University of Alberta

Adam McShane, PhD
Cleveland Clinic

Brian Kelly, PhD, DABCC, FAACC
BNK Clinical Laboratory Consulting, LLC


1. Is There a Better Way to Plan and Perform Experiments? Introduction to Experimental Design
Margrét Thorsteinsdóttir & Finnur Eiriksson

Experimental design (DOE) is a technique that allows to be more efficient in planning, conducting and interpreting results of experiments. In Analytical Chemistry, DOE can be used during method development and optimization, troubleshooting performance and method evaluation. The DOE technique is also very helpful for identifying critical parameters, which have the most significant effect on performance of the methods, products, processes, or systems. The participants will learn why DOE is better than performing experiments by changing one variable at a time, learn about cause-and-effect relationships and interactions between factors. The participants will be introduced to several types of DOE, will learn some of the principles and guidelines for planning experiments.

2. Do Identical Instruments Produce Comparable Patient Results? A Stumbling Block of Harmonizing LC-MS/MS Assays in Clinical Laboratories
Joyce Liao

Clinical mass spectrometry laboratories usually validate individual assays on more than one instrument for continuous operation. Instrument comparison is a requirement of the College of American Pathologists and should be monitored at least twice a year to ensure comparability of results. Although the same style of liquid chromatography-tandem mass spectrometry system is preferred to minimize the variations between instruments, labs will inevitably encounter bias between two or more identical LC-MS/MS systems. Even in the absence of bias, the same instrument model with two different serial numbers may require different instrument settings to obtain similar sensitivity and specificity. In this roundtable session, we will review several comparison data sets from the same extractions injected and analyzed on two LC-MS/MS systems of the same make and model. We will discuss the potential factors, including mass spectrometer hardware (probe type and cleanness) and software settings (gradients, transitions, cone voltages, and collision energies) that could bias patient results and how to establish quality assurance policies to ensure adequate data review and accurate resulting. Examples of challenges we have faced and approaches we have found useful will be presented as a starting point for discussion.

3. Challenges and Possible Solutions on Direct Metabolome Profiling for Clinical Applications
Liang Li

Metabolomics is mainly used for disease biomarker discovery with an objective of translating newly discovered metabolite biomarkers into clinical applications. On the other hand, direct metabolome profiling of human biofluids may be used for monitoring health status of individuals on a population scale. However, there are a number of challenges in realizing this goal. This roundtable discussion will focus on exchanging views on several key areas of technical development that are needed to bring large-scale metabolome profiling to clinical settings. These include sample type, sample handling, analytical platforms, data processing, clinical metabolome informatics, cost and scale, etc.

4. Thyroid Function Testing Interference and How Mass Spectrometry Can Help: Interactive Case Studies
Adam McShane

Thyroid disease affects approximately 20 million Americans, and can lead to a multitude of symptoms. These are often grouped into 2 categories: hyperthyroidism (e.g., anxiety, weight loss, and sleep loss) and hypothyroidism (e.g. fatigue, weight gain, and forgetfulness). Clinicians rely heavily on biochemical assessment of the thyroid for accurate diagnosis to ensure prompt treatment. Routine thyroid function tests utilize immunoassays for their availability, speed, and automation. However, this testing suffers from a variety of interferences which can delay diagnosis or worse lead to miss diagnosis. Liquid chromatography-mass spectrometry (LC-MS) is a much more specific platform than immunoassays that can be utilized in the investigation of potential inferences. This session will utilize real-life, interactive cases to exemplify common thyroid function testing interferences, investigatory considerations, and how LC-MS can be utilized.

5. A Day in The Life of a Remote Laboratory Director
Brian Kelly

Have you ever considered directing a small clinical laboratory, while continuing, or in addition to, your regular work? Do you want to start on your own, or as part of a larger laboratory staffing group? If so, come chat with me, Brian Kelly. I’m a board-certified clinical chemist and have been working as a remote laboratory director for 10 years. Learn about the differences between directing off-site from on-site directorship as well as some basic requirements for running your own small business. This session will be geared toward a question-and-answer format in hopes that other well-trained scientists will improve the quality of laboratory medicine in smaller settings by considering being a remote laboratory director.

2000
Wednesday
845
935
Molecular Phenomics in Systems, Synthetic, and Chemical Biology
@ Steinbeck Ballroom

John A. McLean, Ph.D.
Department of Chemistry, Vanderbilt University


The human genome project is recognized as being one of the most successful big science projects in modern history. One of the primary motivational underpinnings to undertake the HGP was to better understand what made us human and healthy - and how to use this code to improve the human condition by better understanding disease and potential treatment. While the frontiers of our knowledge expanded dramatically, we also uncovered profound biological complexity that we could not understand. This led to the current frontier in the measurement science of molecular phenomics, to catalog the broad-scale changes in the molecular inventory in cells, tissues, and biological fluids at a specific biological state, or in response to exposures and lifestyle choices. In phenomics, we seek to characterize the comprehensive molecular basis of biology (including DNA, RNA, proteins, lipids, carbohydrates, metabolites, and all of their nuances), in both space (e.g. at a cell, tissue, and organismal level) and time (e.g. healthy versus disease state). This places enormous demands on measurement technologies (including minimal sample preparation, fast measurements, high concentration dynamic range, low limits of detection, and high selectivity) and computational approaches to organize the millions of potential species present in vanishingly small spatial coordinates. The interplay between phenomic datasets and bioinformatics forms the nexus of translating phenomics data into actionable information and understanding.

Advances in computational biology rely heavily on the experimental capacity to make omics measurements, i.e. integrated proteomics, metabolomics, lipidomics, glycomics, among many others. Ion mobility-mass spectrometry (IM-MS) provides rapid (ms) gas-phase electrophoretic separations on the basis of molecular structure and is well suited for integration with rapid (us) mass spectrometry detection techniques. This report will describe the fundamental strategies for IM separations and recent advances in IM-MS integrated omics measurement strategies in the analyses of complex biological samples of interest in systems, synthetic, and chemical biology. New advances in artificial intelligence and machine learning based on developments in internet commerce and astronomy will also be described to approach biological queries from an unbiased and untargeted perspective and to quickly mine these massive datasets. These techniques will be highlighted through selected examples ranging from clinical applications of targeted panels, to the creation of microfluidic human-organs-on-chip to replace animal testing in drug development workflows, to probing the outcomes of fast genetic editing experiments (using CRISPR) in the optimization of synthetic biology for fine and commodity chemical production to potential advances in clinical measurements. While enormous challenges remain, the promise is immense – comprehensive diagnostics and predictive capabilities for health and medicine of importance to society and beyond.

Moderated by:

Christopher Chouinard, PhD
Clemson University

2002
Wednesday
935
945
Intermission
@ Steinbeck Foyer
2004
Wednesday
945
1020
Advancing Neuroscience Research via Novel Application of Ion Mobility Mass Spectrometry (IM-MS)
@ Steinbeck Ballroom

Lingjun Li, PhD
School of Pharmacy and Department of Chemistry, University of Wisconsin - Madison


Naturally occurring D-amino acid substitution, also known as amino acid D-isomerization, has been observed in many disease-associated peptides and proteins, including amyloid beta (Aβ), one of the putative biomarkers and drug targets for Alzheimer’s disease. Aβ is of significant interest due to the prevalence of post-translational D-isomerization in AD brain samples. While many prior reports have described technical advancements associated with the chiral discrimination and separation of D-amino acid containing peptides, there remains a dearth of tools capable of targeting Aβ42 stereochemistry. Ion mobility-mass spectrometry (IM-MS) has increasingly become an important alternative for the chiral separation of Aβ stereoisomers. IM-MS offers high analytical speed, low sample consumption and the ability to resolve small structural differences in peptide analytes, driven by recent technological advancements in IM-MS. In this talk, I will present a multi-dimensional IM-MS-based structural analysis strategy to facilitate the study of the chiral effects on monomer structure, oligomeric propensity, and receptor binding for Aβ peptides. Furthermore, analytical strategies to enhance peptide epimer differentiation and for site-specific localization of D-amino acid containing peptides will be presented. Finally, I will discuss our recent efforts in developing a high-resolution ion mobility-enabled sn-position resolved lipidomics strategy for probing phospholipid dysregulation in the brain of a mouse model for Alzheimer’s disease.

Moderated by:

Christopher Chouinard, PhD
Clemson University

2003
Wednesday
1025
1100
Differential Ion Mobility Spectrometry: Understanding the Chemistry in the Mass Spectrometer and How That Affects What Is Detected
@ Steinbeck Ballroom

Gary Glish, PhD
University of North Carolina


Differential Ion Mobility Spectrometry (DIMS) is a powerful tool that can help improve targeted detection of analytes using mass spectrometry (MS). DIMS has a number of advantages over more conventional drift type ion mobility techniques, but currently lacks the ability to determine collisional cross-sections. Some of the advantages of DIMS are: it is readily compatible with any type of mass analyzer; it is more orthogonal to MS because the separation is not based just on cross-section; and gas phase chemistry can be used to dramatically affect separation of analytes that are isomeric/isobaric and even have the same cross-section. A very under-appreciated aspect of DIMS is its ability to provide insight into the ionization chemistry and how that chemistry can significantly distort the resulting mass spectrum. This presentation will provide an overview of DIMS, examples of improvement of targeted analysis using DIMS with and without gas phase chemistry, and examples of how DIMS can provide understanding of chemistry occurring in the mass spectrometry experiment that can lead to inaccurate conclusions.

Moderated by:

Christopher Chouinard, PhD
Clemson University

2077
Wednesday
1100
1230
Poster Session 1
@ Exhibit Hall - Serra
2005
Wednesday
1130
1330
Lunch Buffet
@ Exhibit Hall - Serra
2007
1200
1245
Troubleshooting Posters
@ Exhibit Hall - Serra

To be held in the Troubleshooting Poster Presentation Corner (lower left) of the Exhibit Hall.
12:00 @ poster #33a
Optimization of Lead Recovery in Dried Blood Spot (DBS) During Method Development by ICP-MS
Cody Orahoske
University of California San Francisco
12:15 @ poster #34b
Background Interfering Peaks in a Quantitative LC-MS/MS Dihydrotesterone Assay
Luisa Beltran
Imperial College Healthcare NHS Trust, North West London Pathology
12:30 @ poster #34a
Fundamental Characterization of the Separation of Steroid Isomer Pairs by Reversed-phase HPLC Using Polarity Models
Mst Ummul Khair
Cleveland State University
Wednesday
1230
1400
Poster Session 2
@ Exhibit Hall - Serra
2006

Scientific Session 1

Track 1
Steinbeck 1

Adding Value in Standardization of Proteomics Assays

Chair
Zuzana Demianova
PreOmics
2nd
Xin Cong
InterVenn Biosciences

Track 2
Steinbeck 2

Lipidomics Biomarkers

Chair
Anne Bendt
Singapore Lipidomics Incubator (SLING), National University of Singapore
2nd
Frederick Strathmann
MOBILion Systems

Track 3
Steinbeck 3

Toxicology/TDM

Chair
Briana Fitch
University of Southern California
2nd
Brian Kelly
BNK Clinical Laboratory Consulting, LLC

Track 4
Colton

Practical Training

1400
1420
A Simplified Proteomics LC-MRM-MS Assay for Determination of ApoE Genotypes in Plasma Samples
Deema Qasrawi
McGill University-Lady Davis Institute
Extracellular Vesicles Are a Source of Lipid Biomarkers for Breast and Ovarian Cancer Diagnosis
Erika Dorado
Imperial College London
Evaluation of Analytical Techniques for Drug Checking
John Halifax
UCSF
How To Achieve Lower Quantification Limits
Russell Grant
Labcorp
1420
1440
Lessons in Calibration: Peptide versus Protein Based Calibrators
Meng Wang
Vancouver General Hospital
Comprehensive Correlation Analysis of Tumours, Their Metastases and Established Primary Cell Lines by Rapid Evaporative Ionization Mass Spectrometry
Adrienn Molnár
Waters Research Center, ELTE Eötvös Loránd University
Multiplexed Quantification of Venlafaxine and Four Metabolites in Human Plasma
Claire Knezevic
Johns Hopkins University
...
Extended Session
...
1440
1500
Metrologically Traceable Quantification of Three Apolipoprotein E Isoforms in Cerebrospinal Fluid
Andy Hoofnagle
University of Washington
Lipidomics and Biobanking: Challenges of Pre-Analytical Sample Handling
Lisa Hahnefeld
Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Frankfurt, Germany; Pharmazentrum Frankfurt/ Zafes, Institute of Clinical Pharmacology, Goethe University, Frankfurt, Germany
Optimized UPLC-MS/MS Assay for Therapeutic Drug Monitoring in Patients with Adenine Phosphoribosyltransferase Deficiency
Margret Thorsteinsdottir
University of Iceland
...
Extended Session
...
Wednesday
1500
1530
Coffee Break
@ Exhibit Hall - Serra
2012

Scientific Session 2

Track 1
Steinbeck 1

Emerging Technologies for PoC Dx

Chair
Eftychios Manoli
Imperial College London
2nd
Hannah Brown
Washington University School of Medicine in St. Louis

Track 2
Steinbeck 2

Strategies in Lipidomic Assay Design

Chair
Bo Burla
Sling @ National University of Singapore
2nd
Xueheng Zhao
Cincinnati Children’s Hospital Medical Center

Track 3
Steinbeck 3

Immunology

Chair
Maud Gregson
Institut des Sciences Analytiques, Université de Lyon, France
2nd
Christopher Koch
Sanford Health

Track 4
Colton

Practical Training

1530
1550
Multi-Site Development of a Real-Time Breast Cancer Recognition and Tumor Metabolic Phenotyping Platform Using Rapid Evaporative Ionization Mass Spectrometry
Julia Balog
Waters Research Center
Evaluating Matrix-Specific Adduct Formation for Improved Quantitative Accuracy in Nontargeted Lipidomics
Lauren Bishop
University of California, Davis
Redefining Serological Diagnostics with Immunoaffinity Proteomics
Andrei Drabovich
University of Alberta
Data Automation: From Crawling to Running
Dustin Bunch1, Daniel Holmes2, Patrick Mathias3
(1) Nationwide Children's Hospital, (2) St. Paul’s Hospital, (3) University of Washington
1550
1610
Direct Swab Analysis by Desorption Electrospray Ionisation – Mass Spectrometry for Preterm Birth Risk Stratification in Patients with Cervical Shortening
Katia Capuccini
Imperial College London
Comparison of a Novel Lipid-based MALDI-TOF MS Technique to Two FDA-cleared Direct from Blood Culture Diagnostics
Richard Smith
University of Maryland
Multiplexed Targeted LC-MS/MS Assay to Determine Immune Response to a Panel of Winter Viruses
Dan Lane
University of Leicester
...
Extended Session
...
1610
1630
Rapid and Sensitive Protein Quantitation in Biofluids by Paper Spray Mass Spectrometry: Single Instrument Albumin/Creatinine Ratio Measurements
Chris Gill
Vancouver Island University
Cutting Through the Fat(s) in Clinical Fatty Acid GC-MS Analysis: Analytical Accuracy or Clinical Concordance?
Matthew Crawford
Labcorp
Alteration of Human Immunoglobulin Fc-glycosylation Among Chronic Dialysis Patients After SARS-Cov-2 Vaccination Booster and/or Infection with LC-MSMS Analysis
Chia Yi Chou
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taiwan
...
Extended Session
...
Wednesday
1630
1645
Intermission
@ Steinbeck Foyer
2017
Wednesday
1645
1800
Discussion : Tackling Tough Issues in Toxicology LC-MS/MS Method Development
@ Steinbeck 1

Hsuan-Chieh (Joyce) Liao, PhD, NRCC, DABCC
University of Washington

Joshua Hayden, PhD, DABCC, FACB
Norton Healthcare

Heather Stieglitz, PhD, DABCC
The Ohio State University Wexner Medical Center


Confirmatory urine drug testing by liquid chromatography tandem mass spectrometry (LC-MS/MS) remains a corner stone of clinical mass spectrometry testing. This testing offers significant financial and patient care advantages and thus represents an excellent opportunity for new labs looking to establish or expand their LC-MS/MS testing. A clinical laboratory aiming to setup such testing will be faced with what can seem like an overwhelming number of options and decisions, especially if the laboratory is new or has limited experience with LC-MS/MS testing. This interest group discussion aims to help labs navigate these complex decisions by highlighting some approaches used by three clinical chemists who oversee toxicology testing at three medical centers. Topics that will be discussed include 1. choosing what drug classes and analytes (parent drug, metabolites, etc) to include, 2. whether to detect conjugated or unconjugated drugs, and 3. determining appropriate measuring and reportable intervals. This interactive session will begin with an introduction of the topic using case examples of how each speaker approached the issue followed by an open discussion with the audience on the advantages and limitations of different approaches.
2076
Wednesday
1645
1800
Discussion : Translation of Proteomic Assays to the Clinical Laboratory
@ Steinbeck 2

Esthelle Hoedt, Ph.D
PrognomIQ

Angela Mc Ardle, PhD, MSc, BSc
Evosep Biosystems


This workshop will focus on describing strategies for the translation of proteomic markers from a basic science environment to the clinical laboratory. Specifically, presenters will focus on cases studies describing strategies used to translate assays into use prior to the release of CLSI C64. We also aim to propose strategies and considerations clinical scientists should make for the translation of proteomic targets to clinical assays utilizing the most up to date guidance from CLSI C64.
  • Attendees should have some previous experience in proteomic research and/or assay development or have previously attended the Proteomics Short Course or Proteomics Practical Training session.
  • Participants will discuss specific case studies and the process of translating a proteomic assay from a basic research environment to the clinical lab.
  • Attendees will be familiarized with strategies involved in the development and implementation of high quality clinical proteomic assays, drawing from the new CSLI C64 guide and other sources.
2093
Wednesday
1645
1800
Discussion : Career Fair
@ Steinbeck 3
2089
Wednesday
1645
1800
Discussion : Use of Reference Materials for Calibration and Validation in Clinical Mass Spectrometry Applications
@ Colton

Johanna Camara, PhD
NIST


Reference materials (RMs), including certified reference materials (CRMs), are provided by the National Institute of Standards and Technology (NIST) and other RM producers to support global clinical measurement standardization. These materials are available in various forms, including neat powders, solutions, and clinical matrices. The intended RM uses include calibration and validation, depending on the material. Calibration with RMs may provide traceability to higher-order references when incorporated into specific measurement schemes. The choice of which RM to use and how to incorporate it into a measurement system depends on laboratory goals. This roundtable is designed to discuss RM production, availability, and options for incorporating RMs into clinical laboratory measurement applications. Many RMs are ideally suited for mass spectrometry-based measurement procedures. Many matrix matched RMs (blood serum, plasma, urine) are value assigned based on mass spectrometry-based Reference Measurement Procedures (RMPs). These RMPs typically separate and quantify individual metabolites, epimers, or other chemical variations of clinically relevant measurands that are not necessarily separated and detected by other laboratory techniques, such as immunoassays or microbiological assays. RM users may also need to propagate the measurement uncertainty of RM or other calibrator values to measurement results. NIST provides a publicly available online application ABACUS (Apps for Bayesian Analysis of Chemical quantities Using Shiny) intended as a tool for users to use all data from their experiments to calculate results with rigorous estimates of measurement uncertainty.
2019
Wednesday
1645
1800
Discussion : Clinical LC-MS/MS User Training is Lacking : Moving Towards Training and Certification
@ Stevenson 1

Judy Stone, MT (ASCP), PhD, DABCC
Clinical Chemist (retired)


Currently, there exist no formal training programs or licensure prerequisites for medical laboratory quantitative LC-MS/MS method development scientists or technologists. Although rudimentary LC-MS/MS theory may be part of Medical Laboratory Scientist (MLS) or Medical Laboratory Technologist (MLT) programs, quantitative LC-MS/MS method development is highly complex and typically not included. Lab Directors who are authorized to approve LDT methods are not necessarily trained in the details of technical quantitative LC-MS/MS validation.

We see a need for training and certification. This round table aims to get input and discuss a way forward and discussion topics will include:

(1) Training options

  • Traveling Trainer
  • Laboratory Participant Training Sites
(2) Certification
  • how can we move forward?
  • What certification body is best?

This session is geared towards lab directors, lab scientists, and lab technologists. The goal is to get input from the community about what is needed and how the needs can be addressed.

2020
Wednesday
1800
1900
Happy Hour
@ Steinbeck Foyer

Take a moment to catch up with colleagues after a full day and make plans for dinner.
2103
Wednesday
1830
2000
Dinner On Own
@ Off-site
2104
Wednesday
1900
2400
MSACL Hospitality Lounge
@ Portola Club Room

Drinks, snacks, foosball and open mic!
2021

Thursday

Thursday
600
645
Sunrise Tai Chi / Qigong Activity
@ Ferrantes (10th Floor @ Marriott)

Tai Chi and Qigong (pronounced chee-gong) explore the underlying internal practices and approach to fitness training beyond merely moving the body. Ideal for stress reduction this experience involves the interpenetration of mind and body during movement; meaning it can’t just be the mind, or just the body separately. Qigong exercise includes opening energy centers, joints, and tendons. This is achieved in part through abdominal breathing to calm the mind and in turn relax the body in movement. During the relaxation phase you will learn to activate and sense energy kinesthetically in “felt” terms through simple moving practices. Practice session includes Healing Sounds which function in discharging heat and toxins trapped within the fascia around the internal organs. Qigong exercises together with Tai Chi aim to produce a mental and physical sense of balance, centeredness, and harmony.

What to wear: One principle of Tai Chi and Qigong is relaxed, unrestricted movement, therefore casual loose-fitting clothing is recommended. Avoid tight fitting clothing. Skin breathes, body moves freely with more mobility. Remove any belts during practice. Footwear: Flat soled shoes preferable to feel balanced and rooted or connected to the floor, as opposed to high arched athletic shoes or raised heels. Shoes off OK as long as you aren’t subject to easily slipping or sliding.

2084
Thursday
700
800

Breakfast Industry Workshop(s)

Thermo Fisher Scientific
@ Steinbeck 1

New Frontiers for Pathogen Detection using LC-MS

Liquid chromatography-mass spectrometry metabolomics: A new frontier in microbiology diagnostic testing
Ian Lewis, PhD
University of Calgary
Peptidomic classification of Mycobacterial at sub-species resolution
Tony Hu, PhD
Tulane University School of Medicine

Agilent Technologies
@ Steinbeck 2

Pre-Register

Agilent Technologies Breakfast Workshop

Targeted Omics Workflows for Your Lab
Karen Yannell, PhD
Agilent Technologies
Next Generation of Absolute Protein Quantitation in Biological Matrixes Using MRM: Higher Multiplexity – Faster, Robust and Sensitive
Christoph Borchers, PhD
Jewish General Hospital, McGill University Montreal, QC, Canada
Ultra-high sensitivity for targeted proteomics using Evosep-MRM at the single cell level
Angela Mc Ardle, PhD, MSc, BSc
Evosep Biosystems

Thursday
800
845
Coffee Break
@ Exhibit Hall - Serra

Visit the Exhibit Hall for a relaxed tour of the posters and Exhibits while enjoying your morning coffee.
2088
Thursday
800
845
Coffee Roundtables
@ Steinbeck Foyer

Esthelle Hoedt, Ph.D
PrognomIQ

Kara Lynch, PhD, DABCC
University of California San Francisco

Tim Garrett, PhD
University of Florida College of Medicine

Shannon Haymond, PhD
Northwestern University Feinberg School of Medicine

Stephen Master, MD, PhD, FADLM
Children's Hospital of Philadelphia

Prof. Dr. med. Michael Vogeser
University Hospital, LMU Munich

Jessica Colón-Franco, PhD
Cleveland Clinic Foundation


1. Meetup for Black & Brown Scientists
Esthelle Hoedt

There is a growing appreciation for diversity, equity, and inclusion as drivers of excellence in the workplace in general, where Black and Brown scientists are extremely underrepresented. The goal of this roundtable is to ensure a safe place for full and meaningful conversations between Black and Brown Scientists. We will discuss our individual experiences in the analytical and/or clinical laboratory. Despite discrimination and continuing inequities, we have seen members of these communities rise to leadership roles in our societies. Let’s engage and help identify practices with demonstrated success with collaborative dialogue and identify the actions necessary to further positive change.

2. JMSACL Editors-in-Chief
Kara Lynch & Tim Garrett

The current path and future prospects for the journal. How to participate in or initiate Special Issues. Ask questions about submitting to the journal or being a reviewer.

3. Careers in Clinical Chemistry
Shannon Haymond & Stephen Master

4. IVDR : The Regulation Environment in the EU
Michael Vogeser

As of May 2022, the In Vitro Diagnostics Regulation (IVDR) regulates items (such as calibration and QC materials) manufactured and used in individual medical laboratories in the European Union. The interpretation of the IVDR is still controversial on several points.

5. Testing in Dried Spots, Microcollection Devices and Self-Collection: What’s trending?
Jessica Colon-Franco

The COVID-19 pandemic led to an unprecedented increase of digital health visits. This practice is seen as an effective strategy to manage patients with chronic diseases, although the long term sustainability and impact beyond the pandemic is still evolving. Decentralized sample collection for laboratory testing is projected as a major cornerstone to sustain telemedicine efforts, and could increase remote access to testing and outcomes. Although promising, the literature regarding self-collection and outcomes remains scarce. Moreover, only a few laboratories offer testing in these devices likely due to the technical limitations of these devices, and considerably increased technical and regulatory needs. The purpose of this roundtable is to foster a discussion about the potential clinical applications of testing in dried spots and other self-collection devices, some of the existing literature and technology, clinical practice trends, and foreseeable needs and gaps. The format will consist of presenting brief case studies/vignettes related to testing in these devices, followed by guided and open discussions.

2022

Scientific Session 3

Track 1
Steinbeck 1

Interoperative Dx

Chair
Hannah Brown
Washington University School of Medicine in St. Louis
2nd
Eftychios Manoli
Imperial College London

Track 2
Steinbeck 2

Multi-omics

Chair
William Perry
Centers for Disease Control and Prevention
2nd
Erika Dorado
Imperial College London

Track 3
Steinbeck 3

Biomarkers

Chair
Santosh Renuse
Thermo Fisher Scientific
2nd
Erpan Ahat
Eli Lilly and Company

Track 4
Colton

Practical Training

845
905
Intra-operative Margin Assessment by REIMS During Breast Cancer Surgery and Validation Using a Spatio-Temporal Navigated Cautery and Histopathology
Martin Kaufmann
Queen’s University
A Quantitative Assay for Measuring 1000 Metabolites in Serum, Urine and Fecal Samples
David Wishart
University of Alberta
Development of a LC-MS/MS Method for Measurement of Fusion Sarcoma Proteins in Plasma
Anthony Maus
Mayo Clinic
Pediatric Toxicology Testing Strategies: A Case-based Discussion
Kara Lynch
University of California San Francisco
905
925
Integrated Morphometric and Molecular Classification of Central Nervous System Cancers Using a Unified Platform with Picosecond Infrared Laser Mass Spectrometry
Alexa Fiorante
University of Toronto
Decreasing Relapse in Esogastric Cancer by Improved Diagnostic with Spidermass Technology
Léa Ledoux
Laboratoire Prism Inserm U1192
Hunting for Proteoforms in the Discovery and Verification of Novel Biomarkers of Frontotemporal Dementia
Lauren Forgrave
University of British Columbia
...
Extended Session
...
925
945
Molecularly Aware Robotics for Surgery (MARS) – Autonomous Surgical Intervention Using Mass Spectrometry Mapping
Mark Runciman
Hamlyn Centre for Robotic Surgery, Department of Surgery and Cancer, Imperial College London
Mass Spectrometry-Guided Precision Medicine: A New Frontier for Clinical Microbiology
Ian Lewis
University of Calgary
Elevated Serum Butyrate, a Gut Microbial By-product, is Associated with Improved Outcomes in Chronic Kidney Disease Patients
Liam Heaney
Loughborough University
...
Extended Session
...
Thursday
900
945
Exhibitor Feedback Meeting
@ De Anza 1

Time for exhibitors to join with MSACL admin to provide feedback from MSACL 2023 and plan for the future.
2122
Thursday
945
1000
Intermission
@ Steinbeck Foyer
2027

Scientific Session 4

Track 1
Steinbeck 1

Ion Mobility

Chair
Robin Kemperman
Children’s Hospital of Philadelphia
2nd
Carrie Adler
Agilent Technologies

Track 2
Steinbeck 2

Metabolomics 1

Chair
Daniel Globisch
Uppsala University
2nd
Emma Guiberson
Stanford University

Track 3
Steinbeck 3

Microbial Detection

Track 4
Colton

Practical Training

1000
1020
Targeted Quantification of Androgen Metabolites Using Ion Mobility-Mass Spectrometry
Christopher Chouinard
Clemson University
Identification of Metabolic Alterations Associated with Resistance to Immune Checkpoint Inhibitors in Breast Cancer Utilizing Mass Spectrometry Imaging
Keziah Liebenberg
Baylor College of Medicine
Direct, Rapid and Accurate Identification of Sepsis Causative Micro-organisms from Positive Blood Cultures Using A Scout-triggered MRM Proteomics Assay
Maud Gregson
Institut des Sciences Analytiques, Université de Lyon, France
Practical Considerations for Sample Prep Selection: Where Sensitivity, Cost, and Turnaround Time Collide
Adina Badea
Lifespan/Rhode Island Hospital & the Warren Alpert Medical School of Brown University
1020
1040
PFAS Dark Matter and Slippery Cannabis: Disparate Problems with a Similar Path to a Solution
Frederick Strathmann
MOBILion Systems
Chemical Isotope Labeling LC-MS for Plasma Metabolomic Profiling of Different Stages of SARS-CoV-2 Infected Individuals
Xian Luo
The Metabolomics Innovation Centre
Clinical Performance of the Osmotic Shock-MALDI MS Method to Detect Klebsiella pneumoniae Carbapenemase in Clinical Isolates
Je-Hyun Baek
Seegene Medical Foundation (R&D Center for Clinical Mass Spectrometry)
...
Extended Session
...
1040
1100
Leveraging Ion Mobility-Mass Spectrometry for High-throughput Multi-omics
Kelly Hines
University of Georgia
Low Plasma Glutamine is Associated with Poor Outcomes in a Cohort of Acute Heart Failure Patients
Helen Jordan
University of Leicester

Abstract withdrawn by presenter on March 27, 2023

A Single and Rapid Zeno SWATH DIA Proteomic Analysis for Concomitant Identification and AMR Profiling of Micro-organisms from Positive Blood Cultures
Iulia Macavei
Institut des Sciences Analytiques, Université de Lyon, France
...
Extended Session
...
Thursday
1100
1230
Poster Session 3
@ Exhibit Hall - Serra
2032
Thursday
1130
1330
Lunch Buffett
@ Exhibit Hall - Serra
2034
1200
1245
Troubleshooting Posters
@ Exhibit Hall - Serra

To be held in the Troubleshooting Poster Presentation Corner (lower left) of the Exhibit Hall.
12:00 @ poster #32b
∆8-THC by GC-MS: Unresolved Ion Ratio Failure Investigation
Mark Girton
University of Virginia
12:15 @ poster #32a
Insufficient T3 Sensitivity for DBS Testing on SCIEX 6500 MS
Julian Reed
Molecular Testing Labs
12:30 @ poster #33b
Investigation of Vitamin E Ion Suppression Present in Certain Lipemic Serum Specimens
Will Lace
Seattle Children’s Hospital
Thursday
1230
1400
Poster Session 4
@ Exhibit Hall - Serra
2033
Thursday
1400
Exhibits Close
@ Steinbeck Foyer
2035

Scientific Session 5

Track 1
Steinbeck 1

Antibody Analysis

Chair
Ruben Y. Luo
Stanford University
2nd
Meng Wang
Vancouver General Hospital

Track 2
Steinbeck 2

Metabolomics 2

Chair
Kelly Hines
University of Georgia
2nd
Helen Jordan
University of Leicester

Track 3
Steinbeck 3

Endocrine Assay Optimization and Advances

Chair
Lindsay Bazydlo
University of Virginia
2nd
Ramisa Fariha
Brown University

Track 4
Colton

Practical Training

1400
1420
Time to Fly; TOF Mass Spec for Quantitation of Therapeutic Monoclonal Antibodies
Paula Ladwig
Mayo Clinic
High-Throughput Metabolomics in Screening, Triage, Diagnosis and Treatment of Cervical Disease
Maria Paraskevaidi
Imperial College London
Accurate Quantification of Angiotensin Peptides in Human Plasma Using a Multiple Reaction Monitoring LC-MS/MS Method
William Perry
Centers for Disease Control and Prevention
Interpreting Urine Drug Testing Results
Melissa Budelier
TriCore Reference Laboratories
1420
1440
Lack of Unique Tryptic Peptides in Fully-Humanized Adalimumab Impedes Mass Spectrometry-based Quantification
Jocelyn V. Abonamah
Cleveland Clinic
Unique Chemoselective Probes for Discovery and Investigation of Metabolites in Human Samples with Enhanced Mass Spectrometric Sensitivity
Weifeng Lin
Uppsala University
Development and Validation of a LC-MS/MS Bioanalytical Method for Sulfated Progesterone Metabolites in Serum and Plasma
Brett Holmquist
Labcorp
...
Extended Session
...
1440
1500
The Power of Mass Spectrometry in the Care of Patients with Monoclonal Gammopathies
Mindy Kohlhagen
Mayo Clinic
Using Multiple Serum Sample Cohorts with Chemical Isotope Labeling LC-MS to Discover Biomarkers for Rheumatoid Arthritis Disease
Xiaohang Wang
The Metabolomics Innovation Centre
Characterization of Adrenocortical Function in Patients with Severe Infections Using Corticotropin-stimulated Serum Steroid Profiles
Michael Vogeser
University Hospital, LMU Munich
View Slides (PDF)

...
Extended Session
...
Thursday
1500
1515
Intermission
@ Steinbeck Foyer
2114

Scientific Session 6

Track 1
Steinbeck 1

Microsample Analysis

Chair
Michael Gelb
University of Washington
2nd
Gwen McMillin
University of Utah and ARUP

Track 2
Steinbeck 2

Are We Doing This Correctly?

Chair
Frederick Strathmann
MOBILion Systems
2nd
Carrie Adler
Agilent Technologies

Track 3
Steinbeck 3

Data Science

Chair
Stephen Master
Children's Hospital of Philadelphia
2nd
Bo Burla
Sling @ National University of Singapore

Track 4
Colton

Practical Training

1515
1535
Evaluation and Application of Automated Non-contact Reflectance-based Hematocrit Prediction of Dried Blood Spots
Liesl Heughebaert
Ghent University
Feasibility of Routine Therapeutic Drug Monitoring for 5-Fluorouracil. Why Aren’t We Doing It?
Peter Galettis
University of Newcastle
Uncertainty Calculations for Reference Measurement Procedures
Andrea Geistanger
Roche Diagnostics GmbH
Practical Guidance and Examples of Estimating Reference Intervals by Indirect Methods for LDT Mass Spectrometry Assays
Kelly Doyle
University of Utah Health / ARUP Laboratories
1535
1555
Evaluation of Mass Spectrometry Methods for Glycosaminoglycan Biomarker Quantification in Mucopolysaccharidosis and GM1 Gangliosidosis Newborn Dried Blood Spots
Zackary Herbst
University of Washington
Assessment of Therapeutic Humanized IgG2 Antibody Concentrations in Human Plasma Using LC/PRM-MS and Elisa: A Comparative Study
Claudia Gaither
Faculté de médecine vétérinaire, Université de Montréal, Montreal, Canada, and MRM Proteomics
Comparative Analysis of Untargeted Metabolomics Data Processing Software on Large Scale LC-HRMS Datasets
Khyati Mehta
Cincinnati Children’s Hospital/University of Cincinnati
...
Extended Session
...
1555
1615
Assessment of a 60 Biomarker Health Surveillance Panel (HSP) on Whole Blood from Remote Sampling Devices by Targeted LC/MRM-MS and DIA-MS Discovery Analysis
Stephen A. Whelan
Cedars-Sinai Precision Biomarker Laboratories (PBL)
Quantitative Analysis of Small Molecules in DBS with Imprinted Stable Isotope Internal Standards
Tim Garrett
University of Florida College of Medicine
Development of Innovative Mass Spectrometry Pipelines for Improved Cancer Diagnosis and Biomarker Identification Through Feature Extraction
Yanis Zirem
Laboratory Prism Inserm U1192
...
Extended Session
...
Thursday
1615
1630
Intermission
@ Steinbeck Foyer
2115

Scientific Session 7

Track 1
Steinbeck 1

Preparation for Gala

Session CLOSED

Track 2
Steinbeck 2

Imaging / Cell Analysis

Chair
Noortje de Haan
University of Copenhagen
2nd
Xueheng Zhao
Cincinnati Children’s Hospital Medical Center

Track 3
Steinbeck 3

Emerging Tech

Chair
Christopher Chouinard
Clemson University
2nd
William Perry
Centers for Disease Control and Prevention

Track 4
Colton

Microbiome

Chair
Liam Heaney
Loughborough University
2nd
Iulia Macavei
Institut des Sciences Analytiques, Université de Lyon, France

1630
1650
CLOSED Probing 3D Mammalian Co-culture Models of Ovarian Cancer Metastasis for Metabolic Crosstalk Using Imaging Mass Spectrometry
Hannah Lusk
University of California Santa Cruz
Development of Thread Spray Mass Spectrometry for the Identification of Pulmonary Exacerbation Biomarkers in Cystic Fibrosis
Salmika Wairegi
The Ohio State University
Towards Automated Point-of-Care Profiling of the Vaginal Microbiome Using Direct Swab Analysis by Desorption Electrospray Ionisation Mass Spectrometry (DESI-MS)
Eftychios Manoli
Imperial College London
1650
1710
CLOSED Expanding the Tissue-based N-glycan Imaging MS Workflow into a Platform Technology for Glycan Targeted Biofluid and Cellular Diagnostics
Richard Drake
Medical University of South Carolina
Using Microprobe-Capture In-Emitter Elution and High-Resolution Mass Spectrometry for Characterization and Clinical Testing of β2-Transferrin
Ruben Y. Luo
Stanford University
Microbiota-Dependent Metabolomic Changes After Nutritional Intervention During Pregnancy
Emma Guiberson
Stanford University
1710
1730
CLOSED Combined Single Neuron Patch-Clamp/Mass Spectrometry (PatchC-MS) Analyses
John Yates
Scripps Research Institute
Feasibility Analysis of iEndoscope for Real-Time Data Driven Pathology Using Novel MS and Optical Technology
Lauren Ford
Imperial College London
Advanced Metabolomics Analysis Through Chemical Biology Tools for the Selective Investigation of Gut Microbiota-Derived Metabolites
Daniel Globisch
Uppsala University
Thursday
1730
1800
Happy Half-Hour & Gala Check-In
@ Steinbeck Foyer

Enjoy a relaxing moment during check-in for the Closing Gala in the Steinbeck foyer and Jeffers plaza.
2124
Thursday
1800
2030
Dinner & British-Style Trivia Night with Tim
@ Steinbeck 1

Fee-based Pre-Registration Required for Entry

Includes outdoor seating (weather permitting) on adjacent patio between Stevenson 1 and 2. Presentation of the Poster Awards. Closing statements by the Steering Committee Chair.

2039
Thursday
2030
2400
MSACL Hospitality Lounge
@ Portola Club Room

Drinks, snacks, foosball and open mic!
2040

Friday

Friday
700
930
MSACL Sunrise Challenge Walk-Jog to Lover's Point
@ Off-site

Meet on steps at Custom House Plaza in front of Monterey State Historic Park, just outside Portola (ocean-side). Just like last year.

Will follow the Monterey Recreation Trail for about a 5-mile round-trip to Lover's Point. About 2 hours walking, 1 hour running. Or turn back at any point to shorten the distance.

Followed by breakfast burritos in the MSACL Lounge (Portola Club Room).

2043
Friday
745
1000
Farewell Breakfast
@ Portola Club Room

Enjoy a farewell breakfast burrito to either replenish your tanks after the run/walk, prepare for your flight home, or both!
2125