Technology & Data Science for Enhanced Patient Care

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EUROPE 2015

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Presented at 2015_EU

Brief LC Topic Review - The Basics of Modifying Mobile Phase and Stationary Phase Chemistries to Achieve Resolution
Judy Stone
University of California, San Diego Health System

With modern liquid chromatography-tandem mass spectrometry (LC-MSMS)– it is sometimes said that tandem MS alone conveys sufficient selectivity and therefore LC separation is not always necessary. However LC has tremendous capability to improve MSMS quantitation by reducing positive interference from isobaric compounds and negative interference from ion suppression. When you need that additional specificity it is useful to understand how and when stationary and mobile phase chemistries versus gradient, flow rate, column and particle dimensions can be manipulated to improve resolution. We will review the practicalities of optimizing the mobile phase organic component and modifiers and interrogating different stationary phases.

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