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EUROPE 2015

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Presented at 2015_EU

Varying Those Vexing Voltages: Compound Specific Tuning
Daniel Holmes
St. Paul's Hospital, Vancouver

After ensuring your instrument’s resolution and mass accuracy are appropriately set, the next step in developing a quantitative LC-MS/MS multiple reaction monitoring (MRM) assay is to perform compound-specific tuning. Well-defined signal-optimization experiments are used to determine appropriate ion source electronics and gas flow parameters to specifically quantify the compound of interest. This overview will introduce basic concepts of so-called "compound optimization". In other words, we will explain what is meant when people say that they have "developed mass spectrometric method."

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