Technology & Data Science for Enhanced Patient Care

JMSACL Logo

Learning Center

Access additional learning opportunities.

EUROPE 2015

Help Us Reach Our Educational Support Goal of $40,000
Educational Travel Grants supported in part by:
 

Video Viewing Request

You are requesting to view the following presentation:

Presented at 2015_EU

Brief Sample Preparation Topic Review - Extraction Options for Neutral, Non-Polar Analytes in Serum - Say Good-By to Phospholipids
Judy Stone
University of California, San Diego Health System

Extraction protocols that concentrate neutral, non-polar serum analytes to pg/mL lower limits of quantitation and remove enough matrix to produce a robust method are challenging to develop. Serum phospholipids are present at mg/mL concentrations and contain a charged phosphate group and non-polar fatty acids. This amphipathic structure complicates removal of phospholipids during extraction. Insufficient removal of phospholipids may cause substantial LC-MSMS ion suppression and compromised method robustness -- shortened column lifetimes, shifting retention times, high baselines, and decreased MSMS sensitivity. We will review commonly used LC-MSMS extraction protocols for the ability to concentrate non-ionizable analytes while optimally removing serum phospholipids.

Please Enter the email address used to register for the most recent conference and you will be emailed a link that will allow you to view the requested video.

Email: