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Presented at 2015_EU

A Sample Preparation Method Development Case Study - Trials and Tribulations with Serum Testosterone (Part 1)
Grace van der Gugten
Providence Health

Use of liquid chromatography-mass spectrometry for analysis of small molecules in clinical laboratories has increased in the past few years. An important component of developing a mass spectrometry method is the sample preparation technique employed in order to sufficiently clean up the sample without sacrificing analyte recovery. A number of references out there detail sample preparation techniques but which one do you choose? This presentation will detail the thought process behind the sample preparation technique choice for measurement of serum total testosterone in one laboratory, including the balancing act of what you want to, and what you can realistically achieve.
(Courtesy of Deborah French)

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