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EUROPE 2015

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Presented at 2015_EU

Targeted Quantification of 97 Proteins in Dried Blood Spots
Christoph Borchers
UVic Genome BC Proteomics Centre

We report a multiplexed LC-MRM-MS method for the precise quantification of 97 endogenous proteins in human DBS samples, suitable for biomedical research applications. Standard curves were generated for each of the 173 target peptides (representing the 97 proteins) to fully characterize the assay’s analytical merits. Furthermore, the stability of each target peptide was investigated in DBS samples stored at -20°C, 4°C, RT and 37°C over a duration of 154 days. Finally, we will present a simple strategy for managing the hematocrit effect across samples and discuss the implications on the DBS-MRM workflow.

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