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EUROPE 2015

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Presented at 2015_EU

Ferromagnetic Particles as a Rapid and Robust Sample Preparation for Absolute Quantification of Eicosanoids
Anna Catharina Suhr
Laboratory Medicine, Munich (LMU)

We used ferromagnetic particles as a novel technique to deproteinize plasma samples prior to UHPLC-MS/MS analysis for the quantification of important lipid mediators. A combination of "ferromagnetic particle enhanced deproteination" and subsequent "on-line solid phase extraction" realises quick and convenient sample preparation as well as it provides high sensitivity and robustness. We were able to show that this approach allows accurate and precise quantification of seven exemplary eicosanoids (TXB2, PGE2, PGD2, 5-HETE, 11-HETE, 12-HETE, and arachidonic acid). The use of this semi-automated sample preparation facilitates the screening of large study cohorts. In general ferromagnetic particles might smooth the trail to automation in UHPLC-MS/MS.

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