Technology & Data Science for Enhanced Patient Care

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EUROPE 2015

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Presented at 2015_EU

Quantitation of Desmosine in Plasma by LC-MS/MS as Biomarkers for Elastin Degradation
Jody van den Ouweland
Canisius-Wilhelmina Hospital

Desmosine is a promising biomarker for estimating activity of elastin degradation in patients with COPD, although its clinical validity remains uncertain as reliable and sensitive assays are lacking. An LC-MS/MS method for measuring plasma desmosine was developed and validated. Sample preparation consisted of acid hydrolysis, cellulose SPE using D4-DES as IS, followed by C18 chromatography and MS-analysis using SRM. Measuring range was 0.1-10 ng/ml, imprecision <10%, with recoveries within 80-120%. Plasma desmosine levels from COPD patients were significantly higher compared with healthy individuals. The LC-MS/MS assay appears a valuable tool to assess the potential of desmosine as a biomarker for monitoring disease activity in COPD and to study the effect of therapeutic interventions.

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