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Presented at 2015_EU

Linkage-specific Sialic Acid Derivatization for MALDI-TOF-MS Profiling of Glycoconjugates
Noortje de Haan
Leiden University Medical Center

Glycosylation is among the most common co- and post-translational protein modifications, affecting the physiological and biochemical properties of a protein in various ways. High-throughput protein glycosylation analysis may be performed by MALDI-TOF-MS of either released glycans or glycopeptides. This approach, however, biases the analysis of sialylated glycoconjugates by metastable decay and variations in ionization. Here, we present approaches for the linkage-specific sialic acid derivatization of both released glycans and glycopeptides followed by MALDI-TOF-MS analysis. The methods are fast and have an excellent intra- and inter-day repeatability. We envision the use of these methods for the monitoring of protein glycosylation, both in the analysis of biopharmaceuticals and for the detection of glycomic disease biomarkers.

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