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Presented at 2015_EU

Development of Clinical Assays Based on Parallel Reaction Monitoring
Bruno Domon
Luxembourg Clinical Proteomics Center

Targeted proteomics analyses of biomarker are routinely performed on triple quadrupole mass spectrometers, which present limited selectivity, due to low resolution of the mass filters. The targeted analyses of clinical samples carried out on a quadrupole-orbitrap mass spectrometer using parallel reaction monitoring (PRM) showed a significant gain in sensitivity and selectivity. A new acquisition method leveraging the presence of internal standards showed a dramatic improvement of the reliability and the precision of the analyses. The method has a broad applicability to the quantitative analysis of clinical samples, such as lung cancer plasma samples to discriminate the disease stages and subtypes; and to tissue samples to map driver mutations (EGFR and KRAS).

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