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Presented at 2015_EU

Nanoelectrospray High Resolution Mass Spectrometry or Liquid Chromatography High Resolution Mass Spectrometry for Urinary Metabolic Profiling?
Elena Chekmeneva
Imperial College London

We present a practical comparison of nESI-HRMS and reversed-phase UPLC-MS methods for multiplexed targeted and global metabolic profiling of the urine specimens from the INTERMAP study. The test set consisted of 132 randomly selected samples which included 22 pairs of blinded replicated and different types of quality control samples. Selected metabolites were quantified using the stable isotope labelled internal standards (IS). Both methods were validated according to the FDA guidelines. The quantification results, sensitivity and dynamic ranges were assessed and compared between two methods. The classification ability was examined on the basis of the multivariate analysis of the global profiles.

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