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EUROPE 2015

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Presented at 2015_EU

Quantification of Human Receptor Tyrosine-protein Kinase ErbB-2 (HER2) by Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded Breast Cancer Tissue
Axel Ducret
F. Hoffmann-La Roche Ltd

We describe in this study a selected reaction monitoring (SRM) assay for the quantification of HER2 in FFPE tissues. The assay’s six best candidate peptides showed a linear response over a calibration range of 0.012 to 100 fmol on column (R2: 0.99–1.00) and a lower limit of quantification of 0.155 fmol on column. HER2 peptides were quantified in a cohort of 40 breast tumors expressing different HER2 levels (FISH ratio: 0-2, 2-4, 4-10 and >10 respectively). The SRM assay showed good analytical performance and a high agreement with IHC and FISH data. Furthermore, after normalization for tissue sample size, SRM peptide measurements were able to correctly predict 90% of HER2 amplification status as defined by American Society of Clinical Oncology and College of American Pathologists.

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