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Presented at 2015_EU

Eve’s Curse: How Best to Measure Estradiol Across a Broad Spectrum of Reproductive Endocrine Disorders in Women
Michael Wright
Prince of Wales Hospital

Estradiol is arguably the most potent sex steroid in human biology requiring relatively small concentrations to exert large physiological effects in comparison to its precursor, testosterone. However, the delivery of a clinical diagnostic estradiol service is a significant challenge facing many laboratories. In this study we look at a number of sample extraction options, chromatographic techniques and multistage fragmentation (MRM3) using quadrupole linear ion trap instruments to create robust LC-MS methods that meet the requirements of low level measurement. In this presentation we propose three different methods to meet the needs of our diverse patient population.

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