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Presented at 2015_EU

Research into Lysosomal Storage Metabolism Using Plasma Lipid Characterization by LC-MS/MS
Dan Blake

Research into plasma sphingolipids and determining the concentrations of such is of growing importance in the clinical research laboratory, particularly within groups researching Lysosomal Storage Metabolism. Current methods of analysis primarily involve either enzyme activity procedures or derivatization of compounds prior to analysis. Direct analysis of these groups can be complex due to extensive structural homogeneity between individual compounds. We present here a method for a direct multi-compound approach to this analysis, employing modern advances in column technology to produce a rapid and sensitive LC-MS/MS method for these compounds.

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