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EUROPE 2015

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Presented at 2015_EU

Combining Dried Blood Spots with Stable-isotope Tracers to Profile Dynamics of Glucose Metabolism in Human Subjects
Jean-Pierre Trezzi
University of Luxembourg, LCSB

Here, we present time-resolved dynamics of glucose metabolism in blood after oral administration of 13C stable-isotope labeled glucose in human subjects. To extract absolute flux values for glucose production (GP), glucose disappearance (GD) and gluconeogenesis (GNG)on a whole organism scale in diabetes patients, we developed a simple mathematical ODE based dynamic model that considers the main fluxes of (13C labeled) glucose into and out of the blood. In combination with the time-resolved enrichment patterns (Mass Isotopomer Distributions / MIDS) and absolute concentrations of the target metabolites obtained from DBS sampling and GC/MS measurement, we could determine quantitative and robust values for GP and GNG for each studied subject.

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