Using a Stable Isotope-labeled Protein and Corresponding Set of Stable Isotope-labeled Peptides as Comprehensive Quality Controls for Bottom-up Proteomics
University of Washington
Liquid chromatography used in-line with tandem mass spectrometry (LC-MS/MS) has emerged as the dominant analytical platform for bottom-up proteomics. Described herein is a workflow that utilizes a stable isotope-labeled protein and accompanying set of differentially stable isotope-labeled peptides for the purpose of controlling the variation imparted during sample preparation and MS/MS analysis while simultaneously benchmarking tryptic digestion status and incorporating the indexed retention time (iRT) concept.
Presented at MSACL 2016 US