Apolipoprotein C-III Proteoforms as Biomarkers for Changes in Lipid Metabolism
Arizona State University
Proteins can exist in multiple proteoforms in vivo in different physiological and pathological states. In this work, we addressed the potential of utilizing proteoforms as clinical markers, by exploring the association between apolipoprotein C-III (apoC-III) proteoforms and lipid metabolism. We used mass spectrometric immunoassay to perform cross-sectional and longitudinal characterization of apoC-III proteoforms in obese adolescents, and patients with impaired glucose tolerance and type 2 diabetes. We found strong inverse and independent associations between the relative amount of disialylated apoC-III and plasma triglycerides concentrations in all three cohorts. Other measures, such as cholesterol distribution, also correlated with apoC-III proteoforms.
Presented at MSACL 2016 US