Peptide Selection for Amyloidosis Diagnosis and Typing
University of Washington
Laser-capture microdissection (LMD) used in conjunction with tandem mass spectrometry (MS/MS) has been a superior alternative to the previously applied immunohistochemistry assay. However, there are several aspects of current approach can be reconsidered. Here, we applied a systematic sampling MS/MS strategy to unbiased quantify amyloidosis peptides in different samples. We aim to find a subset of amyloid relate peptides that has best discriminate power for amyloidosis diagnosis and sub-typing using FFPE sample. Preliminary results show that we could successfully type and distinguish amyloidosis and normal cases base on the observed intensities of selected peptides.
Presented at MSACL 2016 US