Analytical Characterization of Multiplexed Parallel Reaction Monitoring Assays to Quantify N-linked Glycosite-containing Peptides in Serum
Johns Hopkins University
Protein glycosylation is one of the most common protein modifications, and the quantitative analysis of glycoproteins has the potential to reveal biological functions and their association with disease. However, the high throughput accurate quantification of glycoproteins is technically challenging due to the scarcity of robust assays for their detection and quantification. We developed and characterized 43 multiplexed parallel reaction monitoring (PRM) assays that were used to quantify formerly N-linked glycosite-containing peptides from 37 proteins in serum from prostate cancer patients. The assays were characterized by performance metrics and criteria established by the NCI’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) to facilitate the widespread adoption of the assays in studies designed to confidently detect changes in the relative abundance of these analytes.
Presented at MSACL 2016 US