Matrix-free Imaging of Metabolites and Lipids in Tissues and Bacterial Colonies by LAESI and NAPA Mass Spectrometry
George Washington University
Spatial distributions of metabolites and lipids in biological tissues have been studied for decades by matrix-assisted laser desorption ionization mass spectrometry and secondary ion mass spectrometry. Despite the success of these techniques characterized by high mass capabilities and excellent spatial resolution, respectively, new ionization sources are being sought to provide better quantitation and fewer spectral interferences in the low mass region. Laser ablation electrospray ionization (LAESI) enables the two- and three-dimensional molecular imaging of tissues and bacterial colonies under ambient conditions. Thin tissue sections and subcellular structures (e.g., lamellipodia) on silicon nanopost array (NAPA) platforms are analyzed and imaged by laser desorption ionization mass spectrometry.
Presented at MSACL 2016 US