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Mass Spectrometry Analysis of CNS Specific Oligoclonal Immunoglobulins
Patrick Vanderboom
Mayo Clinic
[email protected]

Isoelectric focusing followed by IgG specific immunoblotting is used to detect immunoglobulins specific to the CNS compartment (oligoclonal banding) and is routinely used as part of the diagnostic criteria for multiple sclerosis. The current isoelectric focusing based reference method is labor intensive and relies on a subjective interpretation of IgG bands from paired CSF and serum. Here, we demonstrate the advantages of a recently developed high throughput mass spectrometry based method which leverages the power of accurate molecular mass to detect and characterize oligoclonal immunoglobulins in CSF.

Presented at MSACL 2016 US