Accelerating the Implementation of Mass Spectrometry in the Clinical Lab

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UNITED STATES 2018

Help Us Reach Our Educational Support Goal of $40,000
Educational Travel Grants supported in part by:

Meet-the-Experts

MSACL strives to offer a congress experience that values training and mentorship (see travel grants, short courses and Practical Training tracks), and provides avenues by which early-career attendees can gain experience presenting (see Poster Lightning Talks, the Poster Contest, Podium opportunities).

But that is not enough. No, it is not. Time for Phase 2.

Our goal now is to create "natural" (see, in the wild) opportunities by which early career attendees can approach, interact and connect with mid- to late-career (aka, grizzled) attendees; such interactions can be the most instructive, and leave the strongest impressions. To this end, we have developed a program called, Meet-the-Experts (aka Meet-the-3XP3rt5, see leet speak). The objective is to provide a mechanism by which experienced attendees can connect with early career participants in a low-pressure, natural setting for scientists, such as browsing Booths, Posters, and/or chatting over drinks.

Introducing ...

MEET-THE-EXPERTS: Booth Tours
MEET-THE-EXPERTS: Poster Tours
MEET-THE-EXPERTS: Office Hours

Expert mentors browse booths or view posters as they normally do, but with one or more mentees along to observe, participate and learn from experience. During Office Hours, Experts will be available at tables in the Meet-the-Experts area of the Exhibit Hall where attendees can sign up to have a 10-15 minute chat about ... anything.

But let's not take ourselves too seriously ...

*The term "expert" is ambiguous, but we're good at some things related to chromatography and mass spectrometry in laboratory medicine.
- Brian Rappold

You can sign up at the conference to participate in one or more Meet-the-Experts activities (i.e., Booth Tour, Poster Tour, Office Hours).

Don't miss this opportunity to establish lasting connections. This is where you can really leverage the common environment of the conference, and the willingness of people to share their knowledge and experience.

Tuesday18:00 - 19:00BOOTH TOURS
Exhibit Hall : Meet-the-Experts Rally Point
Wednesday10:00 - 11:00POSTER TOURS 1
Exhibit Hall : Meet-the-Experts Rally Point
Wednesday13:30 - 14:30POSTER TOURS 2
Exhibit Hall : Meet-the-Experts Rally Point
Wednesday15:45 - 16:45OFFICE HOURS
Exhibit Hall : Meet-the-Experts Rally Point
Thursday10:00 - 11:00POSTER TOURS 3
Exhibit Hall : Meet-the-Experts Rally Point
Thursday12:30 - 13:30POSTER TOURS 4
Exhibit Hall : Meet-the-Experts Rally Point

Erin Baker, PhD
Pacific Northwest National Laboratory

Booth Tour on Tuesday @ 18:00
Poster Tour 1 on Wednesday @ 10:00
Office Hours on Wednesday @ 15:45
Poster Tour 3 on Thursday @ 10:00
Dr. Erin Baker is a bioanalytical chemist with more than 17 years of experience utilizing ion mobility spectrometry in conjunction with mass spectrometry (IMS-MS) to study environmental and biological systems. In the last 12 years she has worked primarily on IMS-MS applications in the field of proteomics and more recently she has optimized IMS-MS metabolomic, glycomic and lipidomic separations. Her research involves the development and evaluation of high throughput IMS-MS, SPE-IMS-MS and LC-IMS-MS analyses to quickly study numerous samples in a short time period without losing valuable biological information, as well as assessing the number and quality of features detected with IMS-MS for comparison with existing MS platforms. Dr. Baker is also presently working with various informatics teams to design and implement software tools that automatically analyzes the complex multidimensional SPE-IMS-MS and LC-IMS-MS data.

Research Interests
· Developing rapid omic separations for complex biological and environmental samples
· Increasing the current dynamic range in mass spectrometry instrumentation for omic analyses
· Uncovering statistically significant molecular changes due to biological conditions and environmental perturbations
· Understanding structural variations between biological molecules in the solution, solid and gas phases

Education and Credentials
Ph.D., Chemistry (specializing in Physical Chemistry), University of California, Santa Barbara, 2001-2005.
B.S., Chemistry (minor in Mathematics), Montana State University, Bozeman, 1997-2001.

Julianne Botelho, PhD
CDC

Office Hours on Wednesday @ 15:45
Julianne is a research chemist and the Team Lead of the CDC Hormone Reference Laboratory and the CDC Hormone and Vitamin D Standardization Programs. In this function, she has developed and maintained higher order reference methods for testosterone and estradiol measurements using LC-MS/MS and manages the operation and the scientific integrity of the Hormone Standardization (HoSt) Program, and Vitamin D Standardization Certification Program (VDSCP) under the direction of Dr. Hubert Vesper. Dr. Botelho also serves on several Clinical and Laboratory Standardization Institute (CLSI) committees to develop standards and guidelines for use of mass spectrometry in the clinical laboratory. Dr. Botelho received her PhD in Analytical Chemistry from the University of Georgia and has been at the CDC in her current role since 2007.

Autumn Breaud
Johns Hopkins University

Office Hours on Wednesday @ 15:45
Autumn Breaud has managed the technical operations for the Johns Hopkins Clinical Chemistry Reference Laboratory of Dr. William Clarke since 2007. During that time, the laboratory has grown from a single-instrument single-assay operation to a full-scale clinical reference and research operation.

Etienne Cavalier, PhD
University of Ličge

Poster Tour 2 on Wednesday @ 13:30
Office Hours on Wednesday @ 15:45
Dr. Etienne Cavalier graduated as Master in Pharmaceutical sciences in 1994 and Advanced Master in Clinical biology in 1999 from the University of Liege (Belgium), which allowed him to be recognized as a European Specialist in Laboratory Medicine (EuSpLM). In 2010, he obtained his PhD thesis at the University of Liege. Dr. Cavalier is now Professor of Clinical Chemistry at the University of Liege and Head of the Department of Clinical Chemistry of the University Hospital of Liege (CHU de Ličge). His current research topics include bone markers, vitamin D, PTH, vascular calcification markers, markers of acute kidney diseases, glomerular filtration rate (estimation, biomarkers), markers of frailty and sarcopenia, LCMS/MS methods for steroids and peptides quantification. Outside of work, although I don’t really have too much time, I have interest in sports (soccer), travels, ecology (I am a Tesla driver :o) and energy. And Belgian beers, wine and gastronomy, of course!



William Clarke, PhD
Johns Hopkins University

Office Hours on Wednesday @ 15:45
Dr. William Clarke is an associate professor of pathology at the Johns Hopkins University School of Medicine. His research focuses on the development of analytical methods for drug analysis, clinical mass spectrometry, and devices for point-of-care testing. Dr. Clarke serves as the director of Clinical Toxicology as well as Critical and Point-of-Care Testing Program at The Johns Hopkins Hospital.

His team’s current projects include qualitative screening for antiretroviral drugs and substances of abuse in various HIV- prevention clinical trials, development and validation of mass spectrometry methods for clinical analysis, and development of clinical assays for use on microfluidic platforms.

Dr. Clarke received his B.S. in Chemistry from the University of Nebraska at Kearney and his Ph.D. in Analytical Chemistry from the University of Nebraska-Lincoln, as well as an M.B.A. from the Carey School of Business at Johns Hopkins University. He completed a post-doctoral fellowship in Clinical Chemistry at the Johns Hopkins School of Medicine.

Tim Collier, PhD
Clevland Heart Lab

Poster Tour 2 on Wednesday @ 13:30
Office Hours on Wednesday @ 15:45
Poster Tour 4 on Thursday @ 12:30
Bachelor Degree in Chemistry obtained from NC State University in Raleigh, North Carolina, with early research experience in Molecular Paleontology under the mentorship of Dr. Mary H. Schweitzer analyzing soft tissue extracts from a 66 million year old T. rex specimen. This early research experience inspired a passion for analytical sciences.

Upon Completion of B.S. Degree in 2006, continued Graduate School at NC State with a focus in Bioanalytical Chemistry and a Graduate Minor in Biotechnology under the mentorship of Dr. David Muddiman at the W. M. Keck FT-ICR Mass Spectrometry Laboratory, working on biological systems ranging from fungal crop pathogens to human embryonic stem cell biology, with methodological focuses on quantitative Top-Down and Bottom-Up Proteomic Methodologies. This exposure to applying new analytical methods to pursue questions related to human health inspired a decision to pursue research in that area further.

After completion of Ph.D. work in 2010, training continued as a Post-Doctoral Research Scholar in the division of Molecular Oncology at Washington University School of Medicine in St. Louis, MO, in the lab of Dr. Ron Bose, and with additional mentorship from Dr. Michael Gross, where quantitative proteomic and siRNA methods were developed to identify drug-resistance mechanisms in HER2 positive breast cancers. Further structural mass spectrometry methods were combined with molecular dynamics simulations to identify specific multimeric interactions between HER2 and HER3 kinases to illuminate structural relationships between tumor-driving mutations and unregulated signaling activity.

Upon completing post-doctoral work in 2013, moved to Cleveland, OH to become a Research and Development Scientist at Cleveland HeartLab, Inc., working with a dynamic team to develop diagnostic tests based on novel biomarkers to improve assessment of cardiovascular risk, and was promoted to current role Senior Research and Development Scientist in 2016.

Bridgit Crews, PhD
UCI Medical Center

Poster Tour 4 on Thursday @ 12:30
Bridgit Crews is an assistant clinical professor at UC Irvine. She currently directs the chemistry, toxicology, and special chemistry sections at UCI Medical Center. She received her PhD in laser spectroscopy and mass spectrometry and became interested in clinical diagnostics while completing a postdoctoral fellowship in Metabolomics at the Scripps Research Institute. She received her clinical training through a COMACC fellowship at the Washington University School of Medicine and subsequently worked as a Technical Director of Toxicology at Kaiser Permanente Regional Laboratories in Berkeley CA, prior to moving to UCI in 2016. She is interested in the development of clinical mass spectrometric assays and most of her current targets are small molecules.

Rob DeWitte, PhD
Thermo Fisher Scientific

Office Hours on Wednesday @ 15:45
Rob is a scientist who has evolved into a business leader over the course of his career. He has a PhD in theoretical Chemical Physics (Harvard University), but has managed to follow his interests into Pharma R&D (startup & Big Pharma), technologies for drug discovery, and most recently platforms for clinical diagnostics. Along the way he has been involved in, or responsible for, R&D, Marketing, Strategy, Business Development, QARA, Manufacturing and General Management. Join Rob in his office hour if you’d like to talk about alternative careers. There are a lot of different paths that lead to interesting opportunities!

Richard Drake, PhD
Medical University of South Carolina

Office Hours on Wednesday @ 15:45
Dr. Drake is a Professor in the Department of Cell and Molecular Pharmacology and Experimental Therapeutics at the Medical University ofSouth Carolina and SmartState Endowed Chair in Proteomics. He is also Director of the Proteomics Center, a mass spectrometry-centric facility with state-of- the-art resources for imaging mass spectrometry, glycobiology and protein PTM applications. He is an experienced protein biochemist and glycobiologist, with particular expertise in MS imaging of tumor tissues and biomarker discovery from clinical fluids. Current efforts are in the large scale application of N-glycan MS imaging methods developed in his laboratory to prostate and breast tumors. Method development of MS imaging approaches for other types of cellular glycans are ongoing, as well as other extracellular matrix targeted assays. The goal is to develop glycan biomarkers associated with tumor, stroma and immune regions of tumors, in two and three dimensions. Further, these glycan maps are being used to identify the localized glycoprotein carriers, and develop new approaches to effectively isolate and characterize tissue glycopeptides. Outside of the lab, Dr. Drake was an early adopter and advocate of the craft beer and microbrewery renaissance, as well as a long time collector of antique Midwest breweriana.

Clifton Fagerquist, PhD
Agricultural Research Service

Office Hours on Wednesday @ 15:45
Poster Tour 3 on Thursday @ 10:00
Dr. Clifton K. Fagerquist received his PhD in physical chemistry from UCLA in 1994 where he used fast atom bombardment and sector-field mass spectrometry to study stable and metastable gas phase clusters. After post-doctoral research at UC Berkeley and two years directing a mass spectrometry facility at University of Minnesota, Minneapolis, he joined the Agricultural Research Service (ARS) in Philadelphia. After two years in Philadelphia, he transferred to the ARS facility in Albany, California where he has been using advanced mass spectrometry and proteomic techniques to identify and characterize foodborne pathogens and their toxins.

Andy Hoofnagle, MD, PhD
University of Washington

Office Hours on Wednesday @ 15:45
Dr. Hoofnagle’s laboratory focuses on the precise quantification of recognized protein biomarkers in human plasma using LC-MRM/MS. In addition, they have worked to develop novel assays for the quantification of small molecules in clinical and research settings. His laboratory also studies the role that the systemic inflammation plays in the pathophysiology of obesity, diabetes, and cardiovascular disease. He also likes to hike and jump up onto large tree stumps in a single bound.

Marilyn Huestis, PhD
Huestis & Smith Toxicology, LLC

Office Hours on Wednesday @ 15:45
Professor Dr. Dr. (h.c.) Marilyn A. Huestis recently retired as a tenured senior investigator and Chief, Chemistry and Drug Metabolism Section, IRP, National Institute on Drug Abuse, National Institutes of Health, after 23 years of conducting controlled drug administration studies. She is an Adjunct Professor, University of Maryland School of Medicine, Baltimore, MD, on the Steering Committee of The Lambert Center for the Study of Medicinal Cannabis and Hemp, Thomas Jefferson Medical School, Philadelphia, PA, on the Smart Approaches to Marijuana (SAM) Science Advisory Board, Senior Scientific Advisor of NMS Labs, Consultant to the US Department of Transportation, and President of Huestis & Smith Toxicology, LLC. Her research program focused on discovering mechanisms of action of cannabinoid agonists and antagonists, effects of in utero drug exposure, oral fluid testing, driving under the influence of drugs, and the neurobiology and pharmacokinetics of novel psychoactive substances. Professor Huestis’ research also explored new medication targets for cannabis dependence, including oral tetrahydrocannabinol (THC), Sativex, a 1:1 ratio of tetrahydrocannabinol and cannabidiol. She has published 461 peer-reviewed manuscripts and book chapters and more than 500 abstracts were presented at national and international meetings.

Brendan MacLean
University of Washington

Booth Tour on Tuesday @ 18:00
Brendan worked at Microsoft for 8 years in the 1990s where he was a lead developer and development manager for the Visual C++/Developer Studio Project. Since leaving Microsoft, Brendan has been the Vice President of Engineering for Westside Corporation, Director of Engineering for BEA Systems, Inc., Sr. Software Engineer at the Fred Hutchinson Cancer Research Center, and a founding partner of LabKey Software. In this last position he was one of the key programmers responsible for the Computational Proteomics Analysis System (CPAS), made significant contributions to the development of X!Tandem and the Trans Proteomic Pipeline, and created the LabKey Enterprise Pipeline. Since August, 2008 he has worked as a Sr. Software Engineer within the MacCoss lab and been responsible for all aspects of design, development and support in creating the Skyline Targeted Mass Spec Environment and its growing worldwide user community.

Mark Marzinke, PhD
Johns Hopkins University

Office Hours on Wednesday @ 15:45
Dr. Marzinke is Associate Professor of Pathology and Medicine in the Johns Hopkins University School of Medicine. He is the Director of Preanalytics and the General Chemistry Laboratory in the Johns Hopkins Hospital Core Laboratories and the Director of the Clinical Pharmacology Analytical Laboratory in the Division of Clinical Pharmacology. He is Co-Director of the HIV Prevention Trials Network (HPTN) Laboratory Center (LC) and Core leader in the Johns Hopkins Center for AIDS Research. Dr. Marzinke currently sits on the Board of Editors for Clinical Laboratory News, and is on the editorial board for the Journal of Applied Laboratory Medicine. Dr. Marzinke received his Ph.D. in Biochemistry from the University of Wisconsin-Madison, followed by a post-doctoral fellowship in Clinical Chemistry at the Johns Hopkins University School of Medicine. Dr. Marzinke is board certified in Clinical Chemistry by the American Board of Clinical Chemistry. His primary research interests are in the areas of antiretroviral pharmacology, precision medicine, mass spectrometry, and laboratory automation. He is a principal investigator or co-investigator on several NIH-funded grants.

Steve Master, MD, PhD
Children’s Hospital of Philadelphia

Poster Tour 2 on Wednesday @ 13:30
Office Hours on Wednesday @ 15:45
Poster Tour 3 on Thursday @ 10:00
Stephen Master received his undergraduate degree in Molecular Biology from Princeton University, and subsequently obtained his MD and PhD from the University of Pennsylvania School of Medicine. After residency in Clinical Pathology at Penn, he stayed on as a faculty member with a research focus in mass spectrometry-based proteomics as well as extensive course development experience in bioinformatics. He is currently Associate Professor of Pathology and Laboratory Medicine at Children’s Hospital of Philadelphia. One of his current interests is in the applications of bioinformatics and machine learning for the development of clinical laboratory assays. He would play with R for fun even if he weren’t getting paid, but he would appreciate it if you didn’t tell that to his department chair.

Alec Saitman, PhD
Providence Regional Laboratories

Booth Tour on Tuesday @ 18:00
Dr. Saitman received his Ph.D. in Organic Chemistry at the University of California, San Diego where he focused his research on the total synthesis of marine natural products. He completed his fellowship training in Clinical Chemistry and Toxicology in the Department of Pathology at the University of California, San Diego under the guidance of Dr. Robert Fitzgerald. Dr. Saitman is double boarded by the American Board of Clinical Chemistry in clinical chemistry and clinical toxicology. He is currently the director of clinical toxicology and special chemistry at Providence Regional Laboratories in Portland Oregon.

Chris Shuford, PhD
LabCorp

Poster Tour 2 on Wednesday @ 13:30
Chris Shuford, Ph.D., is Associate Vice President for research and development at LabCorp’s Center for Esoteric Testing in Burlington, North Carolina. Chris received his B.S. in Chemistry & Physics at Longwood University and obtained his Ph.D. in Bioanalytical Chemistry from North Carolina State University under the tutelage of Professor David Muddiman, where his research focused on applications of nano-flow chromatography for multiplexed peptide quantification using protein cleavage coupled with isotope dilution mass spectrometry (PC-IDMS). In 2012, Chris joined LabCorp’s research and development team where his efforts have focused on development of high-flow chromatographic methods (>1 mL/min) for multiplexed and single protein assays for clinical application.

Sandi Spencer, PhD
University of Washington

Poster Tour 2 on Wednesday @ 13:30
Office Hours on Wednesday @ 15:45
Sandi is currently a postdoctoral researcher in the MacCoss lab at the University of Washington working on method development for targeted proteomics and improvement of nanoLC robustness. She earned her Ph.D. from the University of North Carolina at Chapel Hill working in the Glish lab on real-time analysis of compounds in organic aerosol particles.

Jennifer Van Eyk, PhD
Cedars-Sinai Medical Center

Booth Tour on Tuesday @ 18:00
Poster Tour 1 on Wednesday @ 10:00
The new Advanced Clinical BioSystems Research Institute’s motto is “from discovery to patient care” underlies the essence and foundation of our research. Our research focuses i) on understanding the molecular mechanism underlying acute and chronic disease and the development of precision therapies and ii) in the development of clinically robust circulating biomarkers for personalization of medical care. The central philosophy of our laboratory is that compelling biological and clinical questions drive innovation through development, optimization and adaption of proteomic technologies, functional analysis, and large-scale data handling. We specialize in developing robust technological pipelines and automation systems to precisely quantify proteins and their post-translational modifications (PTM) in disease pathways. This includes understanding interplay between competing PTMs like phosphorylation and multiple oxidative modifications. Our group uses automation in sample preparation to allow for high-throughput and robust MS analysis, which includes discovery and the novel approach, Data independent acquisition-MS (sometimes known as SWATH) that allows complete and reproducible analysis of 1000s of proteins in 100s of samples. As well, absolute quantification of key targets can be quick and cost effective via ultra-sensitive ELISA platforms (e.g. Quanterix) or multiple reaction monitoring (MRM) assay, a targeted MS-based quantitative method, for tracking multidimensional signaling pathways in tissue and body fluids. Based on this, we are expanding to develop the Cedars Sinai Precision Biomarker Labs which consists of a population scale service laboratory and also a CLIA laboratory to move novel and established assays through clinical validation to application.

Michael Vogeser, MD, PhD
Hospital of the University of Munich

Office Hours on Wednesday @ 15:45
Dr. Michael Vogeser, MD, is specialist in Laboratory Medicine and senior physician at the Hospital of the University of the Ludwig-Maximilians-University Munich, Germany (Institute of Laboratory Medicine). As an Associate Professor he is teaching Clinical Chemistry and Laboratory Medicine.The main scope of his scientific work isthe application of mass spectrometric technologies in routine clinical laboratory testing as translational diagnostics. Besides method development in therapeutic drug monitoring and endocrinology a further particular field of his work isquality and risk management in mass spectrometry and in clinical testing in general.Michael has published >150 articles in peer reviewed medical journals and is secretary of the German Association of Clinical Chemistry and Laboratory Medicine (DGKL) (2016 – 2019).