Accelerating the Implementation of Mass Spectrometry in the Clinical Lab

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UNITED STATES 2018

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Short Courses

MSACL hosts a diverse offering of Short Courses.

Short Courses will occur over the first three days of MSACL (Sunday, Monday, Tuesday of January 21 - 23).

Courses are NOT replicated on different days. They are single courses that span multiple days.

Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Clinical MS Review
Clinical MS 301
A Comprehensive Review of Clinical Mass Spectrometry Technology & Techniques, including Miniaturization
Jack Henion, PhD
TBD
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
Tuesday
8:00-12:00
Data Science
Data Science 101
Breaking up with Excel: A Newbie's Introduction to the R Statistical Programming Language
Daniel Holmes, MD & Stephen Master, MD, PhD
TBD
Sunday
14:00-18:00
Monday
9:00-1:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
Tuesday
9:00-13:00
General Interest
Presentations 101
How to Maximize Your Influence Through Creating Compelling Presentations
Karen Mahooti, MBA
TBD
Sunday
14:00-18:00
Monday
8:30-12:30
Lunch
Monday
12:30-13:30
Monday
13:30-17:30
Tuesday
8:30-12:30
Laboratory Medicine
Lab Medicine 101
Basics of Laboratory Medicine
Prof. Dr. med. Michael Vogeser
TBD
Not
in
Session
Not
in
Session
Lunch
Monday
12:30-13:30
Monday
13:30-17:30
Tuesday
8:30-12:30
LC-MS
LC-MSMS 101
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Judy Stone, PhD, Lorin Bachmann, PhD & Grace van der Gugten
TBD
Sunday
14:00-18:00
Monday
8:00-12:00
Lunch
Monday
12:00-13:00
Monday
13:00-17:00
Tuesday
8:00-12:00
LC-MSMS 201
Understanding and Optimization of LC-MS/MS to Develop Successful Methods for Identification and Quantitation in Complex Matrices
Robert D. Voyksner, PhD
TBD
Sunday
14:00-18:00
Monday
9:00-1:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
Tuesday
9:00-13:00
LC-MSMS 202
Practical LC-MS Maintenance and Troubleshooting
J. Will Thompson, PhD, Erik J. Soderblom, PhD & Chris Shuford, PhD
TBD
Sunday
14:00-18:00
Monday
9:00-1:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
Tuesday
9:00-13:00
MALDI
MALDI 102
Practical Considerations for MALDI Imaging Mass Spectrometry
Michelle Reyzer, PhD
TBD
Sunday
14:00-18:00
Monday
9:00-1:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
Tuesday
9:00-13:00
Metabolomics
Metabolomics 201
Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis
Timothy Garrett, PhD & Erin Baker, PhD
TBD
Sunday
14:00-18:00
Monday
8:30-12:30
Lunch
Monday
12:30-13:30
Monday
13:30-17:30
Tuesday
8:30-12:30
Sample Preparation
Sample Prep 201
Sample Preparation and Alternative Matrices for LC-MS Assays
William Clarke, PhD & Mark Marzinke, PhD
TBD
Sunday
14:00-18:00
Monday
9:00-1:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
Tuesday
9:00-13:00



Clinical MS 301 :: A Comprehensive Review of Clinical Mass Spectrometry Technology & Techniques, including Miniaturization

Instructor(s):Jack Henion, PhD
Level:2-3 (Intermediate - Advanced)
Location:TBD
Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Sunday
3:00 - 7:00 pm
Monday
8:00 am -12:00 pm
Monday
12:00 - 1:00 pm
Monday
1:00 - 5:00 pm
Tuesday
8:00am - 12:00pm

Prerequisites: Students who sign up for this advanced course should either have five years or more of personal LC/MS/MS experience and familiarity with the scientific literature or have taken one or more introductory courses which cover atmospheric pressure ionization (API) techniques as well as the basics involved in routine LC/MS and LC/MS/MS analyses.

Overview: This course presents presents a comprehensive overview of technology and techniques of analytical mass spectrometry and from that foundation extends into exciting, disruptive recent developments.

  1. Sample preparation
    • Topics: New types of extraction, Issues to consider, Isolation of proteins from biological samples Ultrafiltration, Affinity techniques, Molecularly Imprinted Polymers (MIP?s), Aptamers, Thermo?s MSIA pipette tips, Electro Extraction, Quechers, SISCAPA, Micro extractions: Dried blood spots (DBS), Dried Plasma Spots (DPS).
  2. Advanced separation techniques for large molecules
    • Topics: UHPLC, Hydrophobic Interaction Chromatography (HIC), Nano-UHPLC, Micro LC/MS, Size Exclusion Chromatography (SEC), ion exchange chromatography, Capillary Electrophoresis (CE), Differential Mobility Spectrometry (DMS).
  3. Ionization techniques for MS
    • Topics: Electrospray ionization (ESI), Nano ESI, Micro ESI, Atmospheric pressure chemical ionization (APCI), Atmospheric pressure photoionization (APPI), Matrix assisted laser desorption ionization (MALDI), LAESI, Electron Ionization (EI) and its potential for LC/MS.
    • To Discuss: New ionization techniques which may be used without on-line separation science technology. This area has evolved into a variety of ambient ionization techniques such as DESI, DART, ASAP, etc.
  4. Mass Analyzers
    • Quadrupoles, Ion traps, linear and quadrupole, Time-of-Flight (TOF), Orbitraps, Hybrid mass analyzer systems, Ion mobility spectrometers, and Differential Mobility Spectrometry (DMS).
    • To Discuss: Developments and improvements in mass analyzers including linear ion traps, FTMS, time-of-flight (TOF), orbitraps, and accelerator mass spectrometry (AMS), the latter currently being applied to micro-dosing experiments by the pharmaceutical industry. Issues such as full-scan acquisition rates, high-resolution mass spectrometry (HRMS), the importance and usefulness of exact mass measurements for qualitative and quantitative analysis, and the analytical merits compared with modern SRM LC/MS experiments will be discussed with many practical examples and applications. The latter will include clinical chemistry issues as well as pharmaceutical, food safety, environmental and industrial examples.
  5. Imaging and profiling by MS
    • Applications of recently reported ionization techniques for imaging the location of chemicals in various matrices employing MALDI, DESI, LAESI, LESA and other techniques.
    • Topics: The technique of MALDI and its applications to tissue imaging as well as DESI, LAESI and also liquid extraction surface analysis (LESA) employing nano-electrospray. A comparison of the various classes of compounds where MALDI and nano ESI provide complimentary coverage of certain compounds found in biological and other matrices.
  6. High resolution MS
    • Topics: Fundamentals, Mass Defects, Isotopic patterns, Mass axis calibration, Types of HRMS systems, Qual/Quan Analysis, Data mining processes, Future directions
    • To Discuss: The analytical merits of HRAMS from QTOF as well as orbitraps and FTMS systems will be presented. Instances where either SRM LC/MS or LC HRAMS may be preferred for optimal selectivity due to chemical background or other interference issues.
  7. Miniaturization in MS
    • Topics: Purdue University "Mini 11", Torion, Microsaic, Advion expression CMS, Waters QDa
    • To Discuss: The benefits and limitations of smaller analytical instrumentation systems will be compared. This includes miniaturization of HPLC systems as well as the mass spectrometers themselves. The commercial introduction of chip-based HPLC systems closely integrated with mass spectrometers offers a glimpse of future directions in analytical chemistry.
  8. Synergistic Integration
    • A systematic overview via specific examples with applications highlighting noted examples of innovative novel and non-standard technologies which demonstrate the analytical potential of new analytical technologies.
    • Developing instrumentation and technologies will be important aspects of future mass spectrometry techniques and its expansion to important new applications. An extremely important example is the need for LC/MS bioanalysis (quantitation) of biologics (ADC?s, large molecules, RNA, etc.) in biological samples employing both bottom up and top down methods. HRAMS coupled with „protein friendly? chromatography will significantly expand our present analytical capabilities. Ion mobility spectrometry (IMS) and transportable mass spectrometers could lead to point-of-care applications and other far reaching applications of mass spectrometry beyond what we are doing today. The future is very exciting!

EarlyBird
Deadline
Nov 29, 2017
Regular
Deadline
Dec 13, 2017
After
Dec 13, 2017
Student / Post-Doc (Trainee)$110$132$165
Academic / Non-Profit$330$396$495
Commercial / Industry$550$660$825
*Short Course Registration is separate from Conference Registration.


Instructor: Jack Henion, PhD

Jack Henion is Professor Emeritus of Toxicology at Cornell University in the Analytical Toxicology Section of the Diagnostic Laboratory within the College of Veterinary Medicine. He is also a co-founder, Chairman and CSO of Advion BioSciences (ABS), Inc., located in Ithaca, New York. Dr. Henion's work in LC/MS relates to the analysis of real-world samples common to pharmaceutical, environmental, and biochemical problems. The instructor has published extensively in the areas of conventional capillary GC/MS as well as LC/MS, SFC/MS, IC/MS, and CE/MS using atmospheric pressure ionization (API) technologies with quadrupole, ion trap, and time-of-flight mass spectrometers.



Data Science 101 :: Breaking up with Excel: A Newbie's Introduction to the R Statistical Programming Language

Instructor(s):Daniel Holmes, MD & Stephen Master, MD, PhD
Level:1-2 (Beginner - Intermediate)
Location:TBD
Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Sunday
3:00 - 7:00 pm
Monday
9:00 am - 1:00 pm
Monday
1:00 - 2:00 pm
Monday
2:00 - 6:00 pm
Tuesday
8:00am - 12:00pm

Prerequisites:

  • knowledge of Excel
  • able to bring a laptop
  • able to pre-install software on their laptop...Namely: R and R-studio
  • willingness to break up with Excel

Overview: Have you ever tried to do Deming regression in Excel only to discover that it is not available? Have you had your figure rejected by a journal because the resolution was not good enough? Have you wished that you could figure out a way to stop manually transcribing your LC-MS/MS results into the LIS?

Well, your wait is over because this year at MSACL we will be offering a course for complete programming newbies that will help you get going analyzing real data related to LC-MS/MS assay development, validation, implementation and publication. The only background expected is the ability to use a spreadsheet program. The skills that you will acquire will allow you to take advantage of the many tools already available in the R language and thereafter, when you see that your spreadsheet program does not have the capabilities to do what you need, you will no longer have to burst into tears. You will be empowe-R-ed.

The course will be run over two days and time will be evenly split between didactic sessions and hands on problem solving with real data sets. Drs Holmes and Master will adopt a “no student left behind policy”. Students will be given ample time to solve mini problems taken from real life laboratory work and focused on common laboratory tasks. All attendees will need to bring a laptop with the R language installed R Studio interface installed. Students may use Windows, Mac OSX or Linux environments. Both R and R studio are free and open-source. No cash required.

Students should be prepared for learning what computer programming is really like. This may involve some personal frustration but it will be worth it.

Obtaining the Software

Instructions for installing the R language are here: http://cran.r-project.org/
Instructions for installing R Studio are here: http://www.rstudio.com/

Course Description

The course will cover:

  • The major types of R variables: vectors (numerical, character, logical), matrices, data frames and lists.
  • The important classes: numeric, character, list and changing between them
  • Importing data from Excel
  • Dealing with non-numeric instrument data: the “<10”s and “>1000”s.
  • Manipulating your data: sub-setting, which, match, sort, unique, cut
  • Simple statistical tests: mean, median, quantiles (normal ranges), t-tests, ANOVA, Wilcoxon.
  • Data merges: matching rows between sets
  • Exporting data to Excel-like format.
  • Regressions: Ordinary Least Squares,Passing Bablok, Deming, weighted regressions.
  • Non-linear regressions
  • Looping: Doing things repeatedly
  • Writing your own functions
  • Making highly customized graphs: scatter plots, regression lines, histograms, box plots, qq-plots
  • Putting it all together projects:
    • Preparing method comparison regression and Bland Altman plots
    • Preparing mass spectrometry data for upload to LIS.

EarlyBird
Deadline
Nov 29, 2017
Regular
Deadline
Dec 13, 2017
After
Dec 13, 2017
Student / Post-Doc (Trainee)$125$150$188
Academic / Non-Profit$375$450$563
Commercial / Industry$625$750$938
*Short Course Registration is separate from Conference Registration.


Co-Instructor: Daniel Holmes, MD

Clinical Associate Professor, Department of Pathology and Laboratory Medicine, University of British Columbia

Daniel Holmes did his undergraduate degree in Chemical Physics from the University of Toronto with a focus on Quantum Mechanics. He went to medical school at the University of British Columbia (UBC) where he also did his residency in Medical Biochemistry. He is a Clinical Associate Professor of Pathology and Laboratory Medicine at UBC and Division Head of Clinical Chemistry at St. Paul's Hospital in Vancouver. Interests include laboratory medicine statistics, clinical endocrinology, clinical lipidology and clinical mass spectrometry. Assay development efforts in the last two years have focused on assays specialized endocrine testing.

Co-Instructor: Stephen Master, MD PhD

Associate Professor of Pathology and Laboratory Medicine, Weill Cornell Medical College

Stephen Master received his undergraduate degree in Molecular Biology from Princeton University, and subsequently obtained his MD and PhD from the University of Pennsylvania School of Medicine. After residency in Clinical Pathology at Penn, he stayed on as a faculty member with a research focus in mass spectrometry-based proteomics as well as extensive course development experience in bioinformatics. He is currently Associate Professor of Pathology and Laboratory Medicine at Weill Cornell Medicine in New York City, where he serves as Director of the Central Lab and Chief of Clinical Chemistry Laboratory Services. One of his current interests is in the applications of bioinformatics and machine learning for the development of clinical laboratory assays. He would play with R for fun even if he weren't getting paid, but he would appreciate it if you didn't tell that to his department chair.



Presentations 101 :: How to Maximize Your Influence Through Creating Compelling Presentations

Instructor(s):Karen Mahooti, MBA
Level:1-2 (Beginner - Intermediate)
Location:TBD
Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Sunday
3:00 - 7:00 pm
Monday
8:30 am - 12:30 pm
Monday
12:30 - 1:30 pm
Monday
1:30 - 5:30 pm
Tuesday
8:00am - 12:00pm

Prerequisites: Working knowledge of presentation software. While this course uses PowerPoint, the methods taught can be used with almost any presentation software.

Overview:

Day 1: Walk through interactive training in the full methodology for storyboarding, visual design, and oral delivery.

Day 2: The best way to learn is by doing! This is a hands-on workshop opportunity to apply the methodology in development of an actual presentation document with the benefit of coaching from the instructor in combination with tools and techniques from the first day of the course. Each participant should bring a topic for a presentation they would like to develop for future use, as well as a general idea of its basic content. You are welcome to bring a laptop if your content is on the computer or needs to be accessed online, but a computer is not required. You will leave the class with a fully developed storyboard and sketched plan for slide visuals.

You have brilliant ideas with great potential. But it doesn't matter what you know; it matters what you communicate! Knowing how to create and deliver clear and compelling presentation documents can unlock influence and opportunity in your career. And the good news is that because there are so many bad presentations out there, armed with the skills in this course, it will be easy for you to stand out in a good way.

This class teaches you how to develop compelling presentations using storyboarding - a method used by professionals in major management consulting firms and some of the largest companies in the world. This versatile method will work for you whether you are in academics, business, government, or a non-profit and whether your audience is executives, colleagues, students, or investors.

While the course has a LOT of very practical information and tips, it is not a collection of sound bites and "top 10 tricks." It is a complete, proven process for creating memorable, persuasive presentations and shows you step by step exactly how to:

  • Structure your content to flow smoothly and logically from start to finish in a way that is highly influential
  • Design professional quality slides that make your key points jump off the page. Visual polish goes a long way in establishing credibility. And yes, YOU can do this, even if you are not a graphic designer!
  • Orally deliver your presentation in a way that fully engages your audience with your ideas.

Many presentation courses are designed for the person giving a keynote address in front of a large auditorium. But most presentations are created for everyday settings: executive or team meetings, classrooms, and client discussions. And these presentations have very different challenges, which are the focus of this course. They must organize and communicate much larger amounts of information, but in a way that is still engaging and easy to follow. In addition, while they may serve as visual aids for an oral presentation, they must also stand on their own two feet and be clearly understood whether or not you are there to narrate them.

Comments from previous year's course participants:

"Very useful and practical. It can be applied right away."

"It was very well laid out and easy to understand. The audience interaction was great and very engaging."

"The materials we get to take home are so well organized and so clear that I was able to use the method on my own, 4 months after the short course."

"I felt very prepared when I had to make a presentation on a new topic. I spent far less time creating graphics I didn’t use and re-arranging slides with Karen’s method. Ultimately that led to less stress during the process of preparing the slides, and it improved the clarity and strengthened the message of my presentation."

EarlyBird
Deadline
Nov 29, 2017
Regular
Deadline
Dec 13, 2017
After
Dec 13, 2017
Student / Post-Doc (Trainee)$125$150$188
Academic / Non-Profit$375$450$563
Commercial / Industry$625$750$938
*Short Course Registration is separate from Conference Registration.


Instructor: Karen Mahooti, MBA

Karen holds an MBA from the Yale University School of Management and is founder and principal at Articulate Consulting. Karen has provided training in presentation development skills to professionals around the world from executives and managers to analysts, consultants, and graduate students. In addition, over her career as both a management consultant and a marketing professional for a Fortune 20 company, Karen has created a multitude of clear and compelling presentations to help senior executives and board members of large companies make better strategic decisions. She understands first-hand the challenges of creating presentations when the stakes are high and clients' expectations are even higher. Karen's style is both visionary and practical. She seeks to inspire others to have confidence in what they can accomplish with their presentations, and also to give them the concrete know-how and tools they need to immediately begin creating presentations that give them the influence they desire.



Lab Medicine 101 :: Basics of Laboratory Medicine

Instructor(s):Prof. Dr. med. Michael Vogeser
Level:1 (Beginner)
Location:TBD
Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Not in Session Not in SessionMonday
12:30 - 1:30 pm
Monday
1:30 - 5:30 pm
Tuesday
8:00am - 12:00pm

Prerequisites:

Overview: This 1-day course aims to make scientists familiar with the basic concepts of clinical pathology and laboratory medicine. Typical processes and workflows in the various categories of clinical laboratories are described and discussed. Topics that are addressed include in particular:

- Basic technologies (photometry, immunoassays, electrochemical methods, cytometry, immuno-fluorescence, etc.)

- Automation and working characteristics of analyzer configurations including total laboratory automation

-Concept of total testing process including pre- and post-analytical processes

- Performance characteristics

- Quality management

- Regulatory background

- Sample materials

- Clinical decision making, reference ranges, decision levels, diagnostic and clinical algorithms

- Logistics, sample transport

- Economic considerations

- Characteristics of IVD industry

EarlyBird
Deadline
Nov 29, 2017
Regular
Deadline
Dec 13, 2017
After
Dec 13, 2017
Student / Post-Doc (Trainee)$100$120$150
Academic / Non-Profit$300$360$450
Commercial / Industry$500$600$750
*Short Course Registration is separate from Conference Registration.


Dr. Michael Vogeser, MD, is specialist in Laboratory Medicine and senior physician at the Hospital of the University of the Ludwig-Maximilians-University Munich, Germany (Institute of Laboratory Medicine). As an Associate Professor he is teaching Clinical Chemistry and Laboratory Medicine.The main scope of his scientific work isthe application of mass spectrometric technologies in routine clinical laboratory testing as translational diagnostics. Besides method development in therapeutic drug monitoring and endocrinology a further particular field of his work isquality and risk management in mass spectrometry and in clinical testing in general.Michael has published >150 articles in peer reviewed medical journals and is secretary of the German Association of Clinical Chemistry and Laboratory Medicine (DGKL) (2016 – 2019).



LC-MSMS 101 :: Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory

Instructor(s):Judy Stone, PhD, Lorin Bachmann, PhD & Grace van der Gugten
Level:1-2 (Beginner - Intermediate)
Location:TBD
Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Sunday
3:00 - 7:00 pm
Monday
8:00 am -12:00 pm
Monday
12:00 - 1:00 pm
Monday
1:00 - 5:00 pm
Tuesday
8:00am - 12:00pm

Prerequisites:

Overview: Is your laboratory under pressure to purchase an LC-tandem MS or is the ROI you wrote last year haunting you now? This short course is designed for attendees implementing quantitative LC-tandem MS for patient testing who have laboratory medicine experience but no mass spectrometry training - CLS bench analysts, supervisors, R&D scientists, and laboratory directors. Theoretical concepts necessary for a robust implementation of clinical mass spectrometry will be presented – but the emphasis is on practical recommendations for:

  • LC-MS/MS system purchasing
  • site preparation and installation
  • defining preliminary MSMS and LC parameters for your first method
  • selecting and optimizing sample preparation for your first method
  • choosing internal standards, solvents, and water, making reagents and calibrators
  • adjusting sample preparation, LC and MSMS parameters to achieve the desired assay performance
  • establishing data analysis & review criteria and an interface to the LIS
  • pre-validation stress testing and method validation
  • preventative maintenance and troubleshooting
  • maintaining quality in production.

Our goal is to present just enough theory so you can report high quality results, while opening a window to the depth and complexity of clinical mass spectrometry such that your appetite is whetted to learn more.

Previous exposure to the principles of clinical method validation, either didactic or practical, is assumed. A glossary of common LC-MSMS terms/acronyms, and diagrams delineating basic LC and MSMS instrument components and functions will be emailed to attendees a week prior to the beginning of the course. This material will also be addressed at the beginning of the course, but the initial learning curve can be steep and review prior to the course will be beneficial if you have absolutely no previous exposure with LC-MSMS.

EarlyBird
Deadline
Nov 29, 2017
Regular
Deadline
Dec 13, 2017
After
Dec 13, 2017
Student / Post-Doc (Trainee)$125$150$188
Academic / Non-Profit$375$450$563
Commercial / Industry$625$750$938
*Short Course Registration is separate from Conference Registration.


Co-Instructor: Judy Stone, MT (ASCP), PhD, DABCC

Judy is Sr. Technical Specialist in the Mass Spectrometry Laboratory at the University of California, San Diego Health Center for Advanced Laboratory Medicine. Her research is focused on small molecule method development, interfacing and automation for clinical LC-MSMS. She was faculty chair for the 2009 AACC certificate program “Using Mass Spectrometry in the Clinical Laboratory” and she teaches a workshop at the annual AACC meeting on “Troubleshooting for LC-MSMS”.

Co-Instructor: Lorin Bachmann, PhD, DABCC

Lorin is Associate Professor of Pathology and Co-Director of Chemistry at Virginia Commonwealth University Medical Center. Dr. Bachmann’s areas of interest include implementation of LCMS in the clinical laboratory and laboratory test standardization. She routinely contributes to development of laboratory practice guidelines and has provided many lectures on practical implementation of clinical mass spectrometry.

Co-Instructor: Grace Van Der Gugten

Grace is LC-MS/MS Applications Development Specialist at St Paul’s Hospital in Vancouver BC. She is passionate about developing the most user friendly and streamlined LC-MS/MS assays as possible for routine use in the Special Chemistry Mass Spec Lab. She loves troubleshooting, especially when the cause of problem has been discovered and the issue solved!



LC-MSMS 201 :: Understanding and Optimization of LC-MS/MS to Develop Successful Methods for Identification and Quantitation in Complex Matrices

Instructor(s):Robert D. Voyksner, PhD
Level:2 (Intermediate)
Location:TBD
Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Sunday
3:00 - 7:00 pm
Monday
9:00 am - 1:00 pm
Monday
1:00 - 2:00 pm
Monday
2:00 - 6:00 pm
Tuesday
8:00am - 12:00pm

Prerequisites: General knowledge of laboratory techniques associated with HPLC and mass spectrometry and some hands-on experience with running an LC/MS system.

Overview: This course is designed for the chromatographer / mass spectrometrist who want to be successful in developing methods, method optimization and solving problems using LC/MS/MS. The course covers the atmospheric pressure ionization (API) techniques of electrospray, pneumatically assisted electrospray and atmospheric pressure chemical ionization (APCI) and atmospheric pressure photo ionization (APPI) using single quadrupole, triple quadrupole, time-of-flight and ion trap mass analyzers.

Discussions of sample preparation and chromatography will target method development and optimization for the analysis of "real-world" samples by LC/MS/MS.

The course highlights the following topics with respect to optimization a method to achieve the best sensitivity, specificity and sample throughput:

  1. Optimization ionization in API techniques,
  2. understanding and minimizing matrix suppression,
  3. relative merits of various LC column lengths, particle sizes and column diameters for LC/MS/MS analysis,
  4. introduction into the interpretation of MS/MS spectra,
  5. important issues in LC/MS/MS quantitation, and
  6. optimization of an quantitative analysis.

Applications of LC/MS/MS to analyze compounds of clinical interest in biological matrices will be discussed throughout the course to emphasize the topics covered.

EarlyBird
Deadline
Nov 29, 2017
Regular
Deadline
Dec 13, 2017
After
Dec 13, 2017
Student / Post-Doc (Trainee)$125$150$188
Academic / Non-Profit$375$450$563
Commercial / Industry$625$750$938
*Short Course Registration is separate from Conference Registration.


Instructor: Robert D. Voyksner, PhD

Dr. Robert D. Voyksner received his B.S. in Chemistry at Canisius College in 1978 and his Ph.D. at the University of North Carolina at Chapel Hill in 1982. He was employed at Research Triangle Institute (RTI) from 1983-2001 as the director of the mass spectrometry facility and has been responsible for developing extraction, separation and mass spectrometric methods for biologically and environmentally significant compounds. His work earned him the Presidents Award, the highest award within RTI. In 2001 he co-founded LCMS Limited in Raleigh, NC and has been the President of the company to date. Under his direction LCMS Limited is working on technological advancements in LC/MS, offering services to pharmaceutical, clinical and agrochemical industry for solving unique problems by LC/MS/MS and offering training in LC/MS/MS and MS/MS interpretation and on LC/MS/MS instrumentation. Dr Voyksner is also an Adjunct professor at the North Carolina a School of Vetinary Medicine and at The University of North Carolina School of Pharmacy.

Dr. Voyksner's research in mass spectrometry has resulted in over 230 publications and presentations, primarily in the area of HPLC/MS. He has served on the Board of Directors for The American Society For Mass Spectrometry (ASMS), is on the organization committee for The Montreux LC/MS Symposium and was the organizer for the 1995, 1999, 2003 and 2007 Montreux LC/MS Symposia. Dr. Voyksner has taught over 100 courses on LC/MS, CE/MS and CID interpretation during the past 10 years for ASMS, pharmaceutical companies; ISSX, PBA, HPCE and HPLC focused meetings.



LC-MSMS 202 :: Practical LC-MS Maintenance and Troubleshooting

Instructor(s):J. Will Thompson, PhD, Erik J. Soderblom, PhD & Chris Shuford, PhD
Level:2 (Intermediate)
Location:TBD
Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Sunday
3:00 - 7:00 pm
Monday
9:00 am - 1:00 pm
Monday
1:00 - 2:00 pm
Monday
2:00 - 6:00 pm
Tuesday
8:00am - 12:00pm

Prerequisites: Experience with LC-MS systems.

Overview: The general goal of the course is to enable practitioners of LC-MS/MS in the clinical laboratory to quickly recognize and diagnose specific problems with instrumentation, in order to decrease downtime and cost of repairs. The course includes ‘best practices’ for instrumentation installation, upkeep and maintenance, practical troubleshooting workflows for LC and MS, and will use problem sessions to reinforce skillsets. Although the course uses examples from specific instrumentation for demonstration, the content is geared to be vendor-neutral and applicable to all LC-MS systems. Additionally, we will provide an opportunity to have instrumentation troubleshooting questions from your laboratory addressed by the facilitators.

Brief outline of course content:

  • General “Best Practices” for Successful LC/MS Operation
    1. Best Practices; Getting Started on the Right Foot
    2. Breaking the System Down
    3. System Suitability! What is it, and how do I properly implement?
  • Focus on Liquid Chromatography
    1. Diagnostics using the “heartbeat” of your Chromatographic system
    2. Key System components and where things go wrong
    3. LC troubleshooting workflow
    4. Maintenance Intervals; service contract or do-it-yourself?
    5. Problem sets
  • Focus on Mass Spectrometry
    1. Discussion of Source, Transfer Optics, Vacuum and how each is critical to your system
    2. MS Troubleshooting workflow
    3. Ion optics cleaning and upkeep; what is ‘charging’?
    4. Problem sets
  • Integrated System
    1. Ionization
    2. System Communications
    3. Multi-vendor configurations
    4. Strategies to simplify
    5. Integrated real-lab problem scenarios and team exercises

While some basics of instrument component operation will be covered, it will be most beneficial to scientists with experience actively using LC-MS/MS as an analysis tool. While an in-depth discussion of how to operate each individual instrument is surely outside the scope of any short course, specific system setups will be used as examples and attendees will be encouraged to ask questions about specific systems in their own laboratories.

EarlyBird
Deadline
Nov 29, 2017
Regular
Deadline
Dec 13, 2017
After
Dec 13, 2017
Student / Post-Doc (Trainee)$125$150$188
Academic / Non-Profit$375$450$563
Commercial / Industry$625$750$938
*Short Course Registration is separate from Conference Registration.


Co-Instructor: Will Thompson, PhD

Dr. Thompson received his Ph.D. in Analytical Chemistry from the University of North Carolina at Chapel Hill under Professor James Jorgenson in 2006. Following his Ph.D. he pursued a career in the application of UHPLC with high resolution mass spectrometry in the Disease and Biomarker Proteomics group of Dr. Arthur Moseley at GlaxoSmithKline, leaving GSK in 2008 to join Dr. Moseley in establishing the Duke Proteomics Core Facility. Dr. Thompson is currently the Assistant Director of the Duke Proteomics and Metabolomics Shared Resource, and a Research Assistant Professor in Department of Pharmacology and Cancer Biology. His research interests involve improving the throughput and sensitivity of unbiased and targeted proteomics and metabolomics approaches, with an eye towards generating assays which are easily translatable to the diagnostic laboratory.

Co-Instructor: Erik J. Soderblom, PhD

Dr. Erik Soderblom currently holds a joint appointment as a Research Scientist at Duke University’s Proteomics and Metabolomics Core Facility and as an Assistant Research Professor in Duke University’s Department of Cell Biology. He received a PhD in Molecular and Structural Biochemistry from North Carolina State University in 2008, prior to joining the Core Facility. His expertise lies in the utilization of various liquid chromatography-mass spectrometry (LC-MS) platforms to support a wide variety of research efforts from small scale basic science studies to larger scale clinical proteomics studies. Dr. Soderblom has over 10 years’ experience in differential expression proteomics using single and multi-dimensional nanoscale capillary chromatography coupled to high-resolution mass spectrometers, targeted proteomic analysis using triple quadrupole mass spectrometers (MRM) or high-resolution mass spectrometers (PRM), and comprehensive analysis of post-translational modifications such as phosphorylation and acetylation. As part of his normal responsibilities, Dr. Soderblom is involved in routine maintenance and troubleshooting of both LC and MS components across various instrument platforms, including design and execution of system suitability analysis to assess platform performance over time.

Co-Instructor: Christopher Shuford, PhD

Chris Shuford, Ph.D., is Technical Director for research and development at LabCorp’s Center for Esoteric Testing in Burlington, North Carolina. Chris received his B.S. in Chemistry & Physics at Longwood University and obtained his Ph.D. in Bioanalytical Chemistry from North Carolina State University under the tutelage of Professor David Muddiman, where his research focused on applications of nano-flow chromatography (<500 nL/min) for multiplexed peptide quantification using protein cleavage coupled with isotope dilution mass spectrometry (PC-IDMS). In 2012, Chris joined LabCorp’s research and development team where his efforts have focused on development of high-flow chromatographic methods (>1 mL/min) for multiplexed and single protein assays for clinical application.



MALDI 102 :: Practical Considerations for MALDI Imaging Mass Spectrometry

Instructor(s):Michelle Reyzer, PhD
Level:1-2 (Beginner - Intermediate)
Location:TBD
Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Sunday
3:00 - 7:00 pm
Monday
9:00 am - 1:00 pm
Monday
1:00 - 2:00 pm
Monday
2:00 - 6:00 pm
Tuesday
8:00am - 12:00pm

Prerequisites: Attendees should have a general knowledge of mass spectrometry, but knowledge of imaging and/or MALDI is not required.

Overview: This course will provide an introduction to the basic concepts involved in running a MALDI Imaging Mass Spectrometry experiment, including key parameters of sample preparation, matrix application, imaging acquisition, instrumental parameters, data analysis, and imaging processing. The course will focus on MALDI mass spectrometry, but other mass spectrometry sources currently used for imaging will be touched on. This course will be presented at the beginner to intermediate level, and will be appropriate for clinicians/pathologists looking to learn more about IMS as well as for mass spectrometrists looking to apply this technology to more clinical samples.

EarlyBird
Deadline
Nov 29, 2017
Regular
Deadline
Dec 13, 2017
After
Dec 13, 2017
Student / Post-Doc (Trainee)$110$132$165
Academic / Non-Profit$330$396$495
Commercial / Industry$550$660$825
*Short Course Registration is separate from Conference Registration.


Instructor: Michelle Reyzer, PhD

Michelle received her BS in Chemistry from the College of William and Mary in Virginia in 1991, and after that worked at the NIH at the National Institute for Alcohol Abuse and Alcoholism (NIAAA) in the Laboratory of Membrane Biochemistry and Biophysics for 5 years. She then went to the University of Texas at Austin where she received a PhD in Analytical Chemistry in 2000 in the laboratory of Jennifer Brodbelt. The was followed by a post-doctoral fellowship at Vanderbilt in the laboratory of Richard Caprioli where she was introduced to MALDI Imaging Mass Spectrometry. She has been focused on the use of MALDI for imaging biological tissues for the past 14 years. Michelle is currently a Research Assistant Professor at Vanderbilt University Medical Center, and also serves as the Associate Director of the Tissue Imaging Core laboratory, where she routinely develops methods for the analysis of small molecules in tissue sections for investigators within Vanderbilt as well as external collaborators. In addition, Michelle oversees the collaboration and service projects for the National Research Resource for Imaging Mass Spectrometry.



Metabolomics 201 :: Application of High Resolution Mass Spectrometry and Metabolomics in Clinical Analysis

Instructor(s):Timothy Garrett, PhD & Erin Baker, PhD
Level:2 (Intermediate)
Location:TBD
Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Sunday
3:00 - 7:00 pm
Monday
8:30 am - 12:30 pm
Monday
12:30 - 1:30 pm
Monday
1:30 - 5:30 pm
Tuesday
8:00am - 12:00pm

Prerequisites: LC/MS hands on experience.

Overview: Metabolomics describes the analysis of the small molecules present in our body and ingested from the surrounding environment (i.e. drugs, pesticides, etc.). The analysis of these metabolites has recently been utilized to discover new markers of disease and perturbed metabolic pathways. Metabolomic analyses can be performed with either targeted or untargeted measurements. In targeted studies, only a small subset of metabolites is analyzed and this prevalent in clinical analyses for measurements such as those for newborn screening. Untargeted measurements, however, study all possible small molecules in a single injection and heavily rely on the use of high resolution mass spectrometry to precisely measure the m/z values across many samples. Untargeted measurements are almost always coupled to either gas or liquid chromatography or ion mobility as the retention time or mobility provides an important secondary distinguishing characteristic of each specific metabolite. It is expected that both targeted and untargeted metabolomic measurements will have an important place in future clinical studies.

Given the growth of metabolomics over the past several years, the use of high resolution mass spectrometry has rapidly progressed. High resolution approaches to measure small molecules offer several advantages for clinical analyses such as confirmation via accurate mass of the precursor and product ions and an evaluation of the isotopic abundance. This short course will cover the application of high resolution mass spectrometry to both quantitative and semi-quantitative analyses with a focus on metabolomics.

Topics in this short course include: mass accuracy for identification, tandem mass spectrometry, quantitative aspects of high resolution mass spectrometry, identifying and measuring the metabolome, statistical analysis, ion mobility spectrometry, and liquid and gas chromatography coupled to high resolution MS.

EarlyBird
Deadline
Nov 29, 2017
Regular
Deadline
Dec 13, 2017
After
Dec 13, 2017
Student / Post-Doc (Trainee)$115$138$173
Academic / Non-Profit$345$414$518
Commercial / Industry$575$690$863
*Short Course Registration is separate from Conference Registration.


Co-Instructor: Timothy Garrett, PhD

TBA

Co-Instructor: Erin Baker, PhD

Dr. Erin Baker is a bioanalytical chemist with more than 16 years' experience utilizing ion mobility spectrometry in conjunction with mass spectrometry (IMS-MS) to study environmental and biological systems. In the last 10 years she has worked primarily on IMS-MS applications in the field of proteomics and more recently she has worked to optimize IMS-MS metabolomic, glycomic and lipidomic separations. Her research involves the development and evaluation of high-throughput IMS-MS, SPE-IMS-MS and LC-IMS-MS analyses to quickly study numerous samples in a short time period without losing valuable biological information, as well as assessing the number and quality of features detected with IMS-MS for comparison with existing MS platforms. Dr. Baker is also presently working with the PNNL Informatics team to design and implement software tools that automatically analyze the complex multidimensional SPE-IMS-MS and LC-IMS-MS data.



Sample Prep 201 :: Sample Preparation and Alternative Matrices for LC-MS Assays

Instructor(s):William Clarke, PhD & Mark Marzinke, PhD
Level:Beginner / Intermediate
Location:TBD
Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
Sunday
3:00 - 7:00 pm
Monday
9:00 am - 1:00 pm
Monday
1:00 - 2:00 pm
Monday
2:00 - 6:00 pm
Tuesday
8:00am - 12:00pm

Prerequisites: Familiarity with LC-MS/MS.

Overview: This course will encompass various sample preparation approaches used for LC-MS assays. The course will highlight not only the importance of sample processing in the clinical laboratory environment, but also illustrate the “fit for purpose” application of processing techniques in clinical mass spectrometry. This course will also discuss the theory behind different specimen preparation methods, strengths and weaknesses of each approach, as well as opportunities for automation. The first 8 hours will serve as a primer of the role of upfront sample management, utilizing examples in blood and urine specimen sources. There will also be an introduction to the application of LC-MS approaches in alternative matrices. The second 8 hours will elaborate on the foundations established in the first half, and expand into newer technologies and automated alternatives for sample processing.

Specific topics to be covered include:

  1. Pain points in clinical LC-MS
  2. Overview of specimen processing in laboratory medicine
  3. Off-line sample processing
  4. On-line sample processing
  5. Analysis of blood and urine
  6. LC-MS of tissue specimens
  7. Alternate body fluid specimens (e.g. CSF, breast milk, etc.)
  8. Intracellular and metabolite analysis
  9. Dried specimens as matrices
  10. Automation of sample processing

Topics will be covered through lecture, Q&A, Case Studies, and small group exercises.

EarlyBird
Deadline
Nov 29, 2017
Regular
Deadline
Dec 13, 2017
After
Dec 13, 2017
Student / Post-Doc (Trainee)$115$138$173
Academic / Non-Profit$345$414$518
Commercial / Industry$575$690$863
*Short Course Registration is separate from Conference Registration.