Accelerating the Implementation of Mass Spectrometry in the Clinical Lab

CMS Logo MSACL

MSACL 2019 EU

Salzburg Sept 22-26

Short Courses

MSACL hosts a diverse offering of Short Courses.

Short Courses will occur over the first three days of MSACL (Sunday, Monday, Tuesday of September 22 - 26).

Courses are NOT replicated on different days. They are single courses that span 1 or 2 days.

Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
LC-MS
LC-MSMS 101 (Beginner)
Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory
Laura Owen, PhD & Michael Wright
Location: TBA
STARTS <
Sunday
14:00-18:00
Monday
9:00-13:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
> ENDS
Tuesday
9:00-12:30
Metabolomics
Metabolomics 202 (Beginner to Intermediate)
Metabolomics: Approaches, Applications and Challenges
Julijana Ivanisevic, PhD & Elizabeth Want, PhD
Location: TBA
STARTS <
Sunday
14:00-18:00
Monday
9:00-13:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
> ENDS
Tuesday
9:00-12:30


LC-MSMS 101 :: Getting Started with Quantitative LC-MS/MS in the Diagnostic Laboratory

Level:Beginner
Prereqs:None.
Location:TBA
Instructor(s):Laura Owen, PhD & Michael Wright

Laura Owen is a Consultant Clinical Scientist at Salford Royal Hospital, where mass spectrometry assays for a variety of small molecule and toxicology services are offered nationally. Prior to working at Salford Laura worked at Wythenshawe Hospital where she has experience of quantitative method development on both LC-MS/MS and exact mass equipment. Laura is a honorary senior lecturer at the University of Manchester, where she lectures on chromatography, mass spectrometry and the adrenal cortex on a national clinical science MSc programme. Laura has also acted as chair of the Association for Clinical Biochemistry’s special interest group for mass spectrometry.

Mike Wright is the Scientific Director at the Drug Development Solutions division of LGC, in Fordham, Cambridgeshire, UK. His team work on the development of assays for the monitoring of drugs and biomarkers, as drug development tools, in a wide variety of matrices. Prior to working at LGC, Mike worked in clinical diagnostics developing LC-MS/MS services for health trusts in the UK and Australia. Mike teaches on LC-MS/MS applications, considerations and troubleshooting and has contributed to a number of online training programs including the AACC’s Introductory Liquid Chromatography Mass Spectrometry certificate.


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
9:00-13:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
> ENDS
Tuesday
9:00-12:30

Overview: Is your laboratory under pressure to purchase an LC-tandem MS or have the instruments arrived and your team are beginning on their mass spectrometry journey? This short course is designed for attendees implementing quantitative LC-tandem MS for patient testing who have laboratory medicine experience but no mass spectrometry training - clinical bench analysts, supervisors, R&D scientists, and laboratory directors. After starting out with a basic chemistry refresher, theoretical concepts necessary for a robust implementation of clinical mass spectrometry will be presented, however, the main focus of the course will be on practical recommendations for:

  • Starting with MSMS, LC and sample extraction parameters
  • Choosing internal standards, solvents, and water, making reagents and calibrators
  • How to fine tune sample preparation, LC and MSMS parameters to achieve the desired assay performance
  • Pre-validation stress testing and method validation
  • Preventative maintenance and troubleshooting
  • Maintaining quality once your new LC-MS/MS assay has gone live
Using real-world examples, recommendations will also be given on:
  • LC-MS/MS system purchasing
  • site preparation and installation (dos and don’ts)
  • establishing data analysis & review criteria and an interface to the LIS

The course will be a steady mixture of lectures, tutorials and problem solving sessions with our goal being to present just enough theory so you can report high quality results, while opening a window to the depth and complexity of clinical mass spectrometry such that your appetite is whetted to learn more. Previous exposure to the principles of clinical method validation, either theoretical or practical, is assumed. A glossary of common LC-MSMS terms/acronyms, and diagrams delineating basic LC and MSMS instrument components and functions will be emailed to attendees a week prior to the beginning of the course. This material will also be addressed at the beginning of the course, but the initial learning curve can be steep and review prior to the course will be beneficial if you have absolutely no previous exposure with LC-MSMS.

EarlyBird
Deadline
Jul 17, 2019
Regular
Deadline
Aug 15, 2019
After
Aug 15, 2019
Student / Post-Doc (Trainee)€84€100€126
Academic / Non-Profit€250$300€380
Commercial / Industry€420€505€630
*Short Course Registration is separate from Conference Registration.



Metabolomics 202 :: Metabolomics: Approaches, Applications and Challenges

Level:Beginner to Intermediate
Prereqs:LC/MS hands on experience.
Location:TBA
Instructor(s):Julijana Ivanisevic, PhD & Elizabeth Want, PhD

Julijana Ivanisevic, PhD is the head of the Metabolomics Research Platform (Senior Lecturer) at the Faculty of Biology and Medicine, University of Lausanne. Her research expertise is in mass spec­trometry-based metabolomics applications to biomedical research (brain metabolism, ageing, cancer metabolism), evolutionary biology and ecology. Her postdoctoral research was at the Center for Metabolomics at The Scripps Research Institute in La Jolla, California, USA.

Elizabeth Want, PhD is a Senior Lecturer in Molecular Spectroscopy in the Department of Surgery and Cancer at Imperial College, London and the Director of the Imperial International Phenome Training Centre. She joined Imperial College in 2006 after working as a postdoctoral researcher at the Scripps Research Institute in La Jolla, CA. Her research at Imperial College involves the development, optimisation and application of LC-MS methodologies for the analysis of biological samples, largely in the context of metabolic phenotyping. She applies these methods to biomedical research areas including toxicology, cardiovascular disease, neonatal disease and development, and neurological diseases.


Course Contact Hours
Sunday PM Monday AM Lunch Monday PM Tuesday AM
STARTS <
Sunday
14:00-18:00
Monday
9:00-13:00
Lunch
Monday
13:00-14:00
Monday
14:00-18:00
> ENDS
Tuesday
9:00-12:30

Overview:

Metabolome: Downstream of Genome

  • Central Dogma of Molecular Biology – From Genotype to Metabotype
  • Historical Perspective of Metabolomics
  • Technological platforms (NMR, GC/MS, LC/MS) and Applications
  • OmicChallenge – Metabolite Diversity

Approaches in Metabolomics

  • Targeted versus Untargeted (work on the front end vs. work on the back end, instrumentation, etc.)
  • Experimental Design and Sample Preparation (depending on the approach)
  • Choice of Analytical Platform (depending on the approach and metabolites of interest)
  • OmicChallenge – « Big » Data Reduction

Data (Pre)Processing in Untargeted Experiments

  • Step by step from peak picking to peak grouping and annotation
  • Open-access platforms
  • Hands-on XCMS Online
  • Omic Challenge – Metabolite Identification

Statistical Analysis, Metabolite Identification and Mapping onto Pathways

  • Univariateversus Multivariate Statistics (SIMCA, MetaboAnalyst)
  • Metabolite Matching against Metabolite Databases (METLIN, HMDB, LIPIDMAPS, MassBank)
  • Metabolite Set Enrichement Analysis and Network Modeling (MetaboAnalyst, KEGG, BioCyc, Mummichog, Ingenuity)
  • Omic Challenge – Integration with other Omic Technologies in a Biologically Relevant Context

EarlyBird
Deadline
Jul 17, 2019
Regular
Deadline
Aug 15, 2019
After
Aug 15, 2019
Student / Post-Doc (Trainee)€84€100€126
Academic / Non-Profit€250$300€380
Commercial / Industry€420€505€630
*Short Course Registration is separate from Conference Registration.